Cobalt carbonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across based on grouping of substances (category approach)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline standard with acceptable restrictions (10 days observation period, no GLP)

Data source

Reference

Reference Type:

publication

Title:

Acute oral toxicity of inorganic cobalt compounds in rats.

Author:

Speijers GJA, Krajnc EI, Berkvens JM, van Logten MJ

Year:

1982

Bibliographic source:

Fd Chem Toxic 20: 311

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

(10 day observation)

GLP compliance:

no

Test type:

standard acute method

Test material

Test material information

Constituent 1

Reference substance name:

10026-22-9

EC Number:

600-049-3

Cas Number:

10026-22-9

IUPAC Name:

10026-22-9

Details on test material:

- Source: E . Merck AG. Darmstadt, FRG
- Name of test material (as cited in study report): Cobalt(II) nitrate hexahydrate
- Molecular formula (if other than submission substance): Co(NO3)2 6H2O
- Molecular weight (if other than submission substance): 291.1
- Physical state: solid
- Analytical purity: 99 %
- Other: Apart from chemically combined water the test substance is identical to CAS 10141-05-6

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: animal breeding unit, National Institute of Public Health, Bilthoven, the Netherlands
- Weight at study initiation: approx. 100 g
- Housing: singly or in pairs of the same sex in wire cages
- Diet: ad libitum (purified diet, Muracon-SSP Tox (Trouw Ltd, Putten)
- Water: ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22-25
- Humidity (%): 35-55
- Photoperiod (hrs dark / hrs light): no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Doses:

450; 675; 1000; 1500; 2250 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 10 days
- Frequency of observations: rectal temperature: 1.5, 24 and 48 hours after treatment
- Necropsy of survivors performed: no
- Other examinations performed: clinical signs, other: rectal temperature

Statistics:

The oral LD50 values of male and female rats combined were calculated according to the method of 'maximum likelihood' according to Finney (1971).

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

691 mg/kg bw

95% CL:

526 - 907

Remarks on result:

other: LD 50 of tested chemical

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

434 mg/kg bw

Remarks on result:

other: Calculated LD50 for anhydrous compound

Mortality:

Individual values not reported.

Clinical signs:

Highest dose level: sedation, diarrhoea, time- and dose-related drop in body temperature varying between 2.5 to 7.5°C

Any other information on results incl. tables

LD50 calculated for Cobalt(II) ion = 140 mg/kg bw.