Cobalt carbonate

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Method according german national standard, no analytical dose verification, no GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Carbonic acid, cobalt salt

EC Number:

231-419-9

EC Name:

Carbonic acid, cobalt salt

Cas Number:

7542-09-8

IUPAC Name:

cobalt(2+) carbonate

Details on test material:

- Name of test material (as cited in study report): Co-carbonat
- Analytical purity: not stated

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: dosage based on stock solution (2.5g/200 ml A. dest). The stock solution was placed on a rotary shaker for 21 hours at 150 rpm and 293K. After 1 hour resting time, the supernatant was used for preparing the test solutions.
- Controls: 1 inoculated sample
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms

Test organisms (species):

Pseudomonas putida

Details on inoculum:

- Laboratory culture: Pseudomonas putida, obtained from DSM (German collection of microorganisms, Goettingen, Germany)
- strain: DSM 50026
- cultured on agar
- Method of cultivation:
- Temperature: 21 ± 1 °C
- nutrient medium: DIN 38412 Part 8
- Pre-culture:
- Test vessel: Erlenmeyer flasks, volume 350 mL, fill volume 100 mL
- inoculated with bacterial suspension at a turbidity of 10 TE/F at 21 ± 1 °C
- incubation time: 7 hours, shaking

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

17 h

Test conditions

Nominal and measured concentrations:

Nominal test concentrations: 0.39, 0.78, 1.56, 3.13, 6.25, 12.5, 25, 50, 100, 200 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): Penicillium vessel, closed
- Material, fill volume: glass, 10 mL
- Aeration: no
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 1 mL of a Pseudomonas suspension adjusted to a turbidity of 10 TE/F

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: AK medium according to DIN 38412, part 8 (draft)

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Photometric determination of light extinction after 17 hours of exposure

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

17 h

Dose descriptor:

other: EC90

Effect conc.:

83.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

17 h

Dose descriptor:

EC10

Effect conc.:

10.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 3

Duration:

17 h

Dose descriptor:

EC50

Effect conc.:

38.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Any other information on results incl. tables

Optical density compared to the control (=100%)

Conc [mg/L]	Optical density	% of control
0.39	0.576	105.52
0.78	0.577	105.98
1.56	0.555	101.33
3.13	0.554	100.92
6.25	0.538	97.61
12.5	0.48	85.55
25	0.444	76.99
50	0.193	28.16
100	0.094	0.83
200	0.205	1.98