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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1966

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: Brown et al. (1966)
Principles of method if other than guideline:
No further information available.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Sulfolane

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
TEST ANIMALS
- Source: Tunstall Laboratory Breeding Unit

Test system

Type of coverage:
occlusive
Preparation of test site:
shaved
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
1 ml
Duration of treatment / exposure:
6 hours, once per day for three applications
Observation period:
7 days after first application
Number of animals:
4 males and 4 females
Details on study design:
TEST SITE
Two 2 cm x 2 cm patches with 1 ml of test material were applied to the backs of rabbits and covered with a sheet of thin polythene. These were then bandaged into position by means of a 5 cm. open-weave bandage.

REMOVAL OF TEST SUBSTANCE
- Not reported.

SCORING SYSTEM: According to Brown et al. (1966).

Results and discussion

In vivo

Results
Irritation parameter:
erythema score
Basis:
other: Score according to Brown et al. (1966)
Time point:
other: 7 days
Score:
< 10
Remarks on result:
other: Non-irritating
Irritant / corrosive response data:
No information is available.
Other effects:
No information is available.

Any other information on results incl. tables

There was no sign of skin irritation in any of the rabbits. The use of sulphan blue showed the skin to be unchanged, and histopathological examination of skins taken from the rabbits post mortem revealed no evidence of skin damage.

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met
Conclusions:
Sulfolane is a non-irritant to rabbit skin.
Executive summary:

Sulfolane is a non-irritant to rabbit skin when applied under occlusive conditions for 6 hours a day on three consecutive days.