2-(octylthio)ethanol

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in vivo (non-LLNA)

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Study final report provided only a brief summary of methods and results; stated that the procedure employed was that descibed by J.H. Draize as published in the "Appraisal of the Safety of Chemicals in Food, Drugs and Cosmetics". No information was provided on controls.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1966

Report date:

1966

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: The procedure employed was that described by J.H. Draize as published in the "Appraisal of the Safety of Chemicals in Food, Drugs and Cosmetics".

Principles of method if other than guideline:

No data

GLP compliance:

not specified

Type of study:

intracutaneous test

Test material

Test material information

Constituent 1

Reference substance name:

2-(octylthio)ethanol

EC Number:

222-598-4

EC Name:

2-(octylthio)ethanol

Cas Number:

3547-33-9

Molecular formula:

C10H22OS

IUPAC Name:

2-(octylsulfanyl)ethan-1-ol

Constituent 2

Reference substance name:

Ethanol, 2-(octylthio)-

IUPAC Name:

Ethanol, 2-(octylthio)-

Constituent 3

Reference substance name:

2-hydroxyethyl-n-octyl sulfide

IUPAC Name:

2-hydroxyethyl-n-octyl sulfide

Details on test material:

- Name of test material (as cited in study report): Repellent 874
- Name of test material- MGK Repellent 874, Technical Grade, consists of 95 % (based on percent of theoretical sulfur content) or more of 2-hydroxyethyl-n-octyl sulfide and 5 % or less of related compounds-all considered active for labeling purposes by the Environmental Protection Agency.
- Stability under test conditions: Stable under all usual conditions of storage and use. Avoid highly alkaline emulsifiers in emulsion concentrates.
- Lot/batch No.: 24

In vivo test system

Test animals

Species:

guinea pig

Strain:

not specified

Sex:

male

Study design: in vivo (non-LLNA)

Induction

Induction 1

Route:

other: intracutaneous

Vehicle:

other: isotonic saline

Induction 2

Concentration / amount:

First induction injection: 0.05 mL of a 0.1% suspension of the test substance.
Remaining nine induction injections: 0.1 mL of the suspension was used.
Challenge exposure-0.05 mL of a 0.1% suspension

Challenge

Challenge 1

Route:

other: intracutaneous

Vehicle:

other: isotonic saline

Challenge 2

Concentration / amount:

First induction injection: 0.05 mL of a 0.1% suspension of the test substance.
Remaining nine induction injections: 0.1 mL of the suspension was used.
Challenge exposure-0.05 mL of a 0.1% suspension

No. of animals per dose:

10

Details on study design:

MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 10 total
- Frequency of applications: 3 times per week (Monday, Wednesday, Friday)
- Concentrations-First induction injection: 0.05 mL of a 0.1% suspension of the test substance.
Remaining nine induction injections: 0.1 mL of the suspension was used.

B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: Two weeks after the tenth primary injection.
- Evaluation (hr after challenge): 24 and 48 hours

Results and discussion

In vivo (non-LLNA)

Results

Results 1

Reading:

1st reading

Hours after challenge:

24

Group:

test chemical

Dose level:

0.1%

No. with + reactions:

2

Total no. in group:

10

Clinical observations:

Two animals showed perceptible erythema while eight showed some or less than perceptible erythema.

Remarks on result:

other: see Remark

Remarks:

Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 0.1%. No with. + reactions: 2.0. Total no. in groups: 10.0. Clinical observations: Two animals showed perceptible erythema while eight showed some or less than perceptible erythema..

Results 2

Reading:

2nd reading

Hours after challenge:

48

Group:

test chemical

Dose level:

0.1%

No. with + reactions:

0

Total no. in group:

10

Clinical observations:

After 48 hours test animals showed no reaction.

Remarks on result:

other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 0.1%. No with. + reactions: 0.0. Total no. in groups: 10.0. Clinical observations: After 48 hours test animals showed no reaction..

Applicant's summary and conclusion

Interpretation of results:

not sensitising

Remarks:

Migrated information

Conclusions:

It was concluded that the test substance was not a sensitizer to guinea pigs.