Thioacetic acid

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

no

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Thioacetic acid

EC Number:

208-063-8

EC Name:

Thioacetic acid

Cas Number:

507-09-5

Molecular formula:

C2H4OS

IUPAC Name:

ethanethioic S-acid

Test material form:

other: liquidl

Details on test material:

- Name of test material (as cited in study report): Thioacetic acid
- batch AOGHJU,
- purity 99.58%.

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Iffa Crédo, 69210 L'Arbresle, France.
- Age at study initiation: approximately 8 weeks old
- Weight at study initiation: 269 ± 3 g for the males and 229 ± 6 g for the females
- Fasting period before study: no
- Housing: in polycarbonate cages (48 cm x 27 cm x 20 cm). Each cage contained one to seven animals of the same sex during the acclimatization period. During the treatment period, the animalswere housed individually
- Diet/ A04 C pelleted diet ad libitum
- Water: Drinking water filtered by a FG Millipore membrane (0.22 micron) was provided ad libitum.
- Acclimation period: at !east 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21 ± 2
- Humidity (%): 30 to 70
- Air changes (per hr): 12
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Type of coverage:

semiocclusive

Vehicle:

unchanged (no vehicle)

Duration of exposure:

24 hours

Doses:

2000 mg/kg

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Clinical signs, mortality and body weight gain were checked for a period of 14 days following the single application of the test substance. All animals were subjected to necropsy.

Statistics:

None

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD0

Effect level:

>= 2 000 mg/kg bw

Mortality:

No death occurred at 2000 mg/kg.

Clinical signs:

other: The general behaviour of the animals were not affected by treatment with the test substance. No cutaneous reactions were observed.

Gross pathology:

No apparent abnormalities were observed at necropsy in all the animals.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

The dermal LD0 of THIOACETIC ACID is equal to or higher than 2000 mg/kg in rats. No signs of toxicity were observed at this dose.

Executive summary:

The acute dermal toxicity of THIOACETIC ACID was evaluated in rats according to OECD (No. 402, 24th February 1987) and EC (92/69/EEC, B.3, 31 st July 1992) guidelines. The study was conducted in compliance with the principles of Good Laboratory Practice Regulations. The test substance was applied to the skin of one group of ten Sprague-Dawley rats (five males and five females). The application was performed with the undiluted test substance at the dose of 2000 mg/kg, taking into consideration that its specific gravity was 1.064. The test site was then covered by a semi-occlusive dressing for 24 hours. Clinical signs, mortality and body weight gain were checked for a period of 14 days following the single application of the test substance.All animals were subjected to necropsy.No death occurred at 2000 mg/kg. The general behaviour and overall body weight gain of the animals were not affected by treatment with the test substance. No cutaneous reactions were observed.No apparent abnormalities were observed at necropsy in all the animals.Under these experimental conditions, the dermal LD₀ of THIOACETIC ACID is equal to or higher than 2000 mg/kg in rats. No signs of toxicity were observed at this dose.