2,4(1H,3H)-Pyrimidinedione, 1-(3,5-di-O-acetyl-2-deoxy-.beta.-L-erythro-pentofuranosyl)-5-methyl-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26.5.2009 - 28.5.2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

Samples for possible analysis were taken from all test concentrations and the control.
Frequency at t=0 h and t=48 h
Volume 1.5 ml from the approximate centre of the test vessels

Vehicle:

yes

Remarks:

Adjusted ISO medium

Details on test solutions:

Preparation of test solutions started with the highest test concentration of 100 mg/L. No special treatment other than careful shaking 5 min of ultrasonic treatment were applied to completely dissolve the test substance in the test medium. The lower test concentrations were prepared by subsequent dilutions in test medium. The final test solutions were all clear and colourless.

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna (Crustacea, Cladocera) (Straus,1820), at least third generation, obtained by acyclical parthenogenesis under specified breeding conditions.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test temperature:

19.2 - 20.2°C

pH:

7.8 - 8.0

Dissolved oxygen:

9.0 - 9.2mg/l

Nominal and measured concentrations:

Nominal test concentrations: 0.1, 1, 10, 100 mg/L
Recovery rate: 97-101%

Reference substance (positive control):

yes

Remarks:

potassium dichromate K2Cr2O7

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

The actual responses in this reference test with K2Cr20 7 were just outside the ranges of the
expected responses. The sensitivity of this batch of Daphnia magna was slightly higher when
compared with the historical data collected at NOTOX. However, the 48h-EC50 was at the lower
limit (0.3 mg/I) as stated in the ISO 6341 and thus acceptable.
The 24h-EC50 was 0.47 mg/I with a 95% confidence interval between 0.44 and 0.53 mg/I.
The 48h-EC50 was 0.28 mg/I with a 95% confidence interval between 0.25 and 0.31 mg/I.

Under the conditions of the present study the test substance did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). The 48h-EC50 exceeded 100 mg/l based on analytically confirmed nominal concentrations.

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions of the present study the test substance did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). The 48h-EC50 exceeded 100 mg/l based on analytically confirmed nominal concentrations.
According to the classification criteria described in REGULATION (EC) No 1272/2008 (CLP) the product has not to be classified.

Description of key information

Under the conditions of the present study the test substance did not induce acute immobilisation of Daphnia magna at 100 mg/l after 48 hours of exposure (NOEC). The 48h-EC50 exceeded 100 mg/l based on analytically confirmed nominal concentrations.

According to the classification criteria described in REGULATION (EC) No 1272/2008 (CLP) the product has not to be classified.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L