1,4-bis(p-tolylamino)anthraquinone

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Data is from experimental lab report

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

other: European Economic Community (EEC), EEC directive 92/69, Part C: Methods for the determination of ecotoxicity, Publication No. L383, December 1992, C.2. "Acute Toxicity for Daphnia".

Principles of method if other than guideline:

The purpose of the toxicity test was to evaluate the influence of test material on the mobility of Daphnia magna.

GLP compliance:

not specified

Analytical monitoring:

not specified

Details on sampling:

No data available

Vehicle:

not specified

Details on test solutions:

test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes.
Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered (Schleicher & Schuell 604 filter) in order to remove the larger (i.e. > circa 5 //m) undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Age of parental stock : < 24 hours

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

observations were made at 24 and 48 h

Hardness:

ISO-medium, hardness: 218 mg CaC03/l

Test temperature:

Between 20.1 °C and 20.8°C

pH:

described in table no.

Dissolved oxygen:

described in table no.

Nominal and measured concentrations:

1.filtered solution prepared at a nominal concentration of 100 mg/I.
2.unfiltered solution prepared at a nominal concentration of 100 mg/l.
3.Blank-control medium

Details on test conditions:

Loading: 10 Daphnia per vessel containing 80 ml test solution
Number of replicates: 2 replicates for each concentration

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

MACROLEX GREEN5B (mg/I)	Vessel number	Number of Daphnia exposed	Response after 24h		Response after 48 h
			Number	%	Number	%
Blank- control	A B	8^3) 10	0(1) 0	0 0	0 0	0 0
100^1)	A^#) B^#)	10 10	0^*) 0^*)	0 0	0^*) 0^*)	0 0
100^2)	A B	10 10	0^1) 0^1)	0 0	0^1) 0^1)	0 0

#):precipitate and floating layer observed prior to the start of the test.

 

‘) :precipitate and floating layer observed

 

I ):precipitate observed.

 

1 ):unfiltered solution.

 

2) : filtered solution.

 

3) : inadvertently 8 instead of 10 Daphnia were exposed in replicate A of the blank-control

Validity criteria fulfilled:

not specified

Conclusions:

Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).

Executive summary:

Test material was used as a test material to evaluate its influence on the mobility of Daphnia magna. test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered in order to remove the larger undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).Based on the NOEC value it can be concluded that test material has no hazardous effect on aquatic invertebrate and can not be classified as per CLP criteria.

Description of key information

Short-term toxicity to aquatic invertebrates:

Test material was used as a test material to evaluate its influence on the mobility of Daphnia magna. test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered in order to remove the larger undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).Based on the NOEC value it can be concluded that test material has no hazardous effect on aquatic invertebrate and can not be classified as per CLP criteria.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

Short-term toxicity to aquatic invertebrates:

To evaluate toxicity of aquatic invertebrates, various experimental studies were performed.Based on the experimental data aquired from various sources following is the discription:

Test material was used as a test material to evaluate its influence on the mobility of Daphnia magna. test material was added to test medium at a nominal concentration of 100 mg/I. The mixture was treated with ultrasonic waves for 5 minutes. Afterwards magnetic stirring was applied for a total period of three days in order to achieve maximum saturation of the test medium with the test substance. After stirring the 100 mg/I solution appeared to be a black turbid dispersion with a floating layer. One part of this 100 mg/I solution was filtered in order to remove the larger undissolved test substance particles. The filtering resulted in a clear greyish coloured solution. The other part of the 100 mg/I solution was not filtered and used as such. Under the conditions of the present study test material did not induce acute immobilisation of Daphnia magna at a nominal concentration of 100 mg/l after 48 hours of exposure (NOEC).Based on the NOEC value it can be concluded that test material has no hazardous effect on aquatic invertebrate and can not be classified as per CLP criteria.

The above study was supported by another study whose aim of the study was to assess the short term toxicity of test chemical to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.The stock solution 10.0 g/l was prepared by dissolving dark violet powder in acetone. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample in reconstituted water. It was not possible to test higher substance concentration due to formation of precipitate.0,0,2,3,4,6,10 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.The median effective concentration (EC50) for the test substance,in Daphnia magna was determined to be 19.7 mg/L with 95% CI:13.4 -28.9 on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic acute/ chronic 3 category as per the CLP classification criteria.

Even though varied results for short term toxicity of aquatic invertebrate were available but, based on first experimental purchased study and maximum notifiers of ECHA , we can consider that the test chemical is non toxic and cannot be classified for aquatic invertebrates.