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EC number: 233-116-7 | CAS number: 10038-98-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Justification for type of information:
- see section 13 of IUCLID for read accross justification
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
- Specific details on test material used for the study:
- PPM Pure Metals GmbH
- Analytical monitoring:
- yes
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The activated sewage sludge sample was maintained on continuous aeration in the laboratory at a temperature of approximately 21 ºC overnight prior to use in the test. On the day of collection the activated sewage sludge (9 liters) was fed synthetic sewage (400 mL). This was a deviation to the study plan as the activated sewage sludge should have been fed at a rate of 50 mL of synthetic sewage per liter of activated sewage sludge but was considered to have had no adverse effects on the study given that all validation criteria were satisfied. The pH of the sample on the day of the test was 7.8 measured using a Hach HQ40d Flexi handheld meter. Determination of the suspended solids level of the activated sewage sludge was carried out by filtering a sample (100 mL) of the activated sewage sludge by suction through a pre-weighed GF/A filter paper* using a Buchner funnel which was then rinsed 3 times with 10 mL of deionized reverse osmosis water and filtration continued for 3 minutes. The filter paper was then dried in an oven at approximately 105 ºC for at least one hour and allowed to cool before weighing. This process was repeated until a constant weight was attained. The suspended solids concentration was equal to 3.0 g/L prior to use.
- Test type:
- static
- Water media type:
- other: synthetic sewage was diluted to 250 mL with water and 250 mL of inoculum added in a 500 mL conical flask
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Remarks on exposure duration:
- Observations were made on the test preparations throughout the test period. Observations of the test item vessels at 0 hours were made prior to addition of activated sewage sludge.
- pH:
- 7.0-8.0 adjusted
- Dissolved oxygen:
- Measured (depletion) measured throughout.
- Nominal and measured concentrations:
- Nominal: 10, 100, 1000 mg GeO2/L
- Details on test conditions:
- A synthetic sewage of the following composition, was added to each test vessel to act as a respiratory substrate:
16 g Peptone, 11 g Meat extract, 3 g Urea, 0.7 g NaCl, 0.4 g CaCl2.2H2O, 0.2 g MgSO4.7H2O, 2.8 g K2HPO4
All dissolved in a final volume of 1 liter of water with the aid of ultrasonication - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Results with reference substance (positive control):
- The reference item gave a 3-Hour EC50 value of 7.6 mg/L, 95% confidence limits 6.0 to 9.7 mg/L.
- Validity criteria fulfilled:
- yes
- Conclusions:
- Test well documented and to be used in classification.
- Executive summary:
The toxicity to Activated Sludge in a Respiration Inhibition Test, using GeO2 revealed a NOEC of 100 mg/L and an EC50 <1000 mg/L.
Reference
Validation Criteria
The coefficient of variation of oxygen uptake in the control vessels in the initial test was 4.1% and the specific respiration rate of the controls was 27.15 mg oxygen per gram dry weight of sludge per hour. The coefficient of variation of oxygen uptake in the definitive test in the control vessels was 3.8% and the specific respiration rate of the controls was 25.66 mg oxygen per gram dry weight of sludge per hour.
The validation criteria have therefore been satisfied.
Description of key information
Study results were available on the toxicity to several bacteria and yeast strains. These studies were conducted with GeO2 according to standard protocols.
The GeO2 No Observed Effect Concentration (NOEC) in the activated sewage sludge respiration test, after 3 hours exposure was 100 mg GeO2/L. The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC50 value of greater than 1000 mg/L. It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 2 040 mg/L
- EC10 or NOEC for microorganisms:
- 204 mg/L
Additional information
In the activated sewage sludge respiration test, the 3 -h NOEC was 100 mg/L (204 mg GeCl4/L), whereas the EC50 > 1000 mg/L; = 2040 mg GeCl4/L.
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