2-Butanol, 2-methyl-, lithium salt (1:1)

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

from 2007-11-09 to 2007-12-07

Reliability:

1 (reliable without restriction)

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)

Version / remarks:

92/69/EEC

Deviations:

no

Principles of method if other than guideline:

NA

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
NA

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, adapted

Details on inoculum:

NA

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

NA

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

Preliminary study:

NA

Test performance:

The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

6.9

Sampling time:

28 d

Details on results:

Under the test conditions the percentage biodegradation of Lithium-tert-amoxide (LTA) reached a mean of 6.9 % after 7 and 28 days based on ThODNH4 (main test) and the test conditions the percentage biodegradation of Lithium-tert-amoxide (LTA) reached a mean of 0.0 % after 7 days based on ThODNH4 (complementary experiment).

BOD5 / COD results

BOD5 / COD

Parameter:

BOD5

Value:

>= 0.15 - <= 0.2 mg O2/g test mat.

Results with reference substance:

The reference item Sodium acetate was sufficiently degraded to a mean of 71.6 % after 14 days, and to a mean of 78.4 % after 28 days of incubation, based on ThODNH4. The percentage biodegradation of the reference item confirms the suitability of the used activated sludge inoculum.

Any other information on results incl. tables

no remarks

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not ready biodegradable

Conclusions:

The test item is considered not ready biodegradable. According to the test guidelines the pass level for ready biodegradability is removal of 60 % ThODNH4.

Executive summary:

Under the test conditions the percentage biodegradation of Lithium-tert-amoxide (LTA) reached a mean of 6.9 % after 28 days based on ThODNH4. Therefore the test item is considered not ready biodegradable.