Amines, di-C12-18-alkyl

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1990

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: according to OECD 203

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Amines, di-C12-18-alkyl

EC Number:

268-899-4

EC Name:

Amines, di-C12-18-alkyl

Cas Number:

68153-95-7

Molecular formula:

R2-NH whereas R = mainly C12-18 (even numbered) alkyl molecular weight range is based on R =12 (lower limit value) and R = 18 (higher limit value)

IUPAC Name:

N-C12-18-(even numbered)-alkyl-1-C12-18-(even numbered)-amine

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): not soluble

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

the test material was homogenized with Ultra Turrax Rotator and ultrasonic and stirred afterwards 2 h;

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Ultra Turrax
- Evidence of undissolved material (e.g. precipitate, surface film, etc): substance was present at bottom and surface in layers

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebrafish
- Strain:
- Source: West aquariom 3422 Bad Lauterberg
- Length at study initiation (length definition, mean, range and SD): 3.0 cm (2.4-3.4 cm) SD = 0.27
- Feeding during test
- Food type: Tetra min, Tetra Werke, Melle
- Frequency: twice a day


ACCLIMATION
- Acclimation period: 14 days
- Acclimation conditions (same as test or not): same

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

21.6-22.5 C

pH:

7.6-8.3

Dissolved oxygen:

7.1-8.7 mg/L

Nominal and measured concentrations:

Nominal: 2.2, 5, 10, 22, 50 mg/L

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

EC0

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

14.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 10-22 mg/L

Effect concentrations 3

Duration:

96 h

Dose descriptor:

EC100

Effect conc.:

22 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The LC50 (9h h) of the test substance was determined to be between 10-22 mg/L.