2-Isobutyryl-3,N-diphenylacrylamide

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1995-02-02 to 1995-08-29

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD study under GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Version / remarks:

92/69/EWG, C.4-E (Geschl. Flasch.)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Details on test material:

- Name of test material (as cited in study report): PD 132408 / BIBEA
- Physical state: White Powder
- Analytical purity: 98.25%
- Impurities (identity and concentrations): not mentioned in the test report
- Composition of test material, percentage of components: not mentioned in the test report
- Lot/batch No.: 409226
- Expiration date of the lot/batch: 1995-07-31
- Storage condition of test material: in darkness at room temperature

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Anglian wter sewage treatment plant, Godmanchester, Great Britain
- Method of cultivation: A sample of activated sludge was collected from the sewage treatment farm on 2 February 1995. The sample wass allowed to settle for approximately 30 minites and the supernatant filtered through Whatman GFA coarse filter paper (first 250 ml discharged).
- Storage conditions: The filtrate was maintained under aeration and used within 24 hours of collection
- Storage length: 24 h at a maximum
- Water filtered: yes
- Type and size of filter used, if any: Whatman GFA coarse filter paper

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

2 mg/L

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: KH2PO4 8.5 mg/l, K2HPO4 21.75 mg/l, Na2HPO4.2H2O 33.4 mg/l, NH4Cl 0.5 mg/l, MgSo4.7H2o 22.5 mg/l, CaCl2 27.5 mg/l, FeCl3.6H2O 0.25 mg/l
- Test temperature: 20 +/- 1°C
- pH: not given
- pH adjusted: no

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Value:

36

Sampling time:

28 d

Details on results:

Points of degradation plot (test substance):
36 % degradation after 4 d
31 % degradation after 7 d
31 % degradation after 11 d
36 % degradation after 14 d
33 % degradation after 18 d
32 % degradation after 21 d
32 % degradation after 25 d
31 % degradation after 28 d

BOD5 / COD results

Results with reference substance:

Points of degradation plot (reference substance):
64 % degradation after 4 d
72 % degradation after 7 d
71 % degradation after 11 d
79 % degradation after 14 d
73 % degradation after 18 d
79 % degradation after 21 d
80 % degradation after 25 d
79 % degradation after 28 d

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other:

Conclusions:

BIBEA is not redily biodegradable.

Executive summary:

In a OECD 301D Closed Bottle test BIBEA was shown to be not readily biodegradable with a degradation of 36% after 28 days. The 10 days window was not passed. The positive control worked as expected.