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EC number: 609-256-3 | CAS number: 365400-11-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 Aug - 19 Aug 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test (Carbon and Ammonium Oxidation))
- Version / remarks:
- 2010
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
- Version / remarks:
- 2008
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Ministerium fur Arbeit, Integration und Soziales des Landes Nordrhein-Westfalen, Leverkusen, Germany
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The exposure medium with the reference substance was prepared by adding 8 mL of the synthetic medium, 100 mL of activated sludge and a defined amount of the stock solution to achieve the test concentrations, and was filled up with deionised water to 250 mL and aerated at 20 ± 2°C.
Before use the wet weight/dry weight relationship of the activated sludge was determined by drying 10 mL of sludge suspension. Subsequently, a sludge suspension of 2 g (dry weight)/L was prepared. The pH of this suspension was measured and adjusted to 6-8. 8 mL of the synthetic medium and 100 mL of activated sludge were added to the dissolved test item. The mixture was filled up with deionised water to 250 mL and aerated at 20 ± 2 °C. To determine the heterotrophic oxidation four additional controls and two replicates with the test item concentration 100 mg/L, all containing 1.25 mL of ATU-solution (N-allylthiourea), which equals to a final concentration of 11.6 mg ATU/L, were prepared.
- Eluate: no
- Differential loading: no
- Controls: Control vessels (inoculated sample without test item) were prepared the same way. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: no
- Name and location of sewage treatment plant where inoculum was collected: aeration tank of a domestic waste water treatment plant (Municipal WWTP Cologne-Stammheim, Germany)
- Method of cultivation: aeration of the activated sludge at 20 ± 2 °C, daily fed with synthetic medium
- Preparation of inoculum for exposure: Sludge was settled and the supernant was decanted. The sludge was centrifuged and the supernant was decanted. A portion of the sludge was dried to obtain a dry weight. The calculated amount of sludge was dissolved in synthetic medium and diluted with deionised water. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 3 h
- Test temperature:
- 20 ± 2 °C
- pH:
- 6-8
- Nominal and measured concentrations:
- nominal: control, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 300 mL glass Erlenmeyer flasks, fill volume: 250 mL, headspace: 50 mL
- Aeration: permanent
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- Sludge concentration (weight of dry solids per volume): 800 mg/L suspended solids
- Nutrients provided for bacteria: synhetic sewage feed: For each liter of water - 16.0 g peptone, 11.0 g meat extract , 3.0 g urea, 0.7 g NaCl, 0.4 g CaCl2 x 2H2O , 0.2 g MgSO4 x 7H2O, and 2.8 g K2HPO4
- Nitrification inhibitor used (delete if not applicable): N-allylthiourea
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
OTHER TEST CONDITIONS
- Adjustment of pH: pH was adjusted to 7.3 - 7.5
- Details on termination of incubation: The aeration was discontinued and the contents of the flask were transferred to 250 mL BOD bottles for oxygen content measurement.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): oxygen consumption as a proxy for respiration rate aftere aeration time of 3 h.
TEST CONCENTRATIONS: control and 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- - Blank controls oxygen uptake rate: 19.264 mg/L-h (with ATU 19.983 mg/L-h)
- Coefficient of variation of oxygen uptake rate in control replicates: 14 % - Results with reference substance (positive control):
- - Results with reference substance valid? The EC50 of the reference compound 3,5-Dichlorophenol should be in the range of 2 - 25 mg/L for total respiration. In the present test the EC50 of the reference compound was 20.171 mg/L; thus the test is valid.
- Reported statistics and error estimates:
- Student-t test for Homogeneous Variances
- Validity criteria fulfilled:
- yes
- Remarks:
- See Table 1 in "Any other information on results incl. tables".
Reference
For details on respiration rates see attachment.
Table 1: Validity criteria for OECD 209
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The blank controls (without the test substance or reference substance) oxygen uptake rate should not be less than 20 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
The oxygen uptake rate in the blank controls was 24.1 mg oxygen per one gram of activated sludge (dry weight of suspended solids) in an hour. |
yes |
The coefficient of variation of oxygen uptake rate in control replicates should not be more than 30% at the end of definitive test. |
The coefficient of variation of oxygen uptake rate in control replicates was 14% at the end of definitive test. |
yes |
Description of key information
EC10 (3 h) > 100 mg/L (activated sludge, OECD 209)
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
- EC10 or NOEC for microorganisms:
- 100 mg/L
Additional information
One experimental study was conducted in accordance with OECD Guideline 209 and EU method C.11 under GLP conditions (M-566988-01-1). The activated sludge was exposed to the test item at a limit test item concentration of 100 mg/L. The respiration rate of each mixture was determined after aeration periods of 3 hours (no nitrification set up was performed). The test item showed 1.4% respiration inhibition of activated sludge at a test item concentration of 100 mg/L. The EC50 (3 h) is higher than 100 mg/L. The NOEC (3 h) is equal or higher than 100 mg/L. The effect value relates to a nominal concentration, since no analytical monitoring was performed. No inhibition of the degradation process in sewage treatment plants is expected.
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