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EC number: 601-489-9 | CAS number: 1176-81-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Nov 1998 - Jul 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- TOC analyser
- Details on sampling:
- The organic carbon concentration of the stock solution was analyzed in a single determination with a TOC analyzer before the start of the incubation.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: For the preparation of the test solutions a suspension with a nominal loading of 102 mg/L test item was stirred for approximately 24 hours. This suspension was filtered through a glassfibre filter. The resulting solution served as the highest concentration (saturated solution). It was further diluted 1:10, 1:100 and 1:1000 with demineralized water.
- Eluate: no
- Differential loading: no
- Controls: yes, test meidum control - Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM:
- Source: "Sammlung von Algenkulturen, Universität Goettingen" (Collection of Algae Cultures, University Goettingen, Germany)
- Maintenance and Acclimatisation: kept on slant agar containing a nutrient solution under standardized conditions (10-15 °C, diffuse light (light/dark rhythm 12 h/12 h)); this culture was dissolved in a liquid nutrient solution and subsequently transferred approx. once weekly
Preparation of pre-cultures
- algae for the pre-culture were taken from a stock up to seven days old, approx. 72 hours before the start of the incubation in the test; kept in the concentrated nutrient solution (diluted 1:10), starting with an approximate cell density of 10,000 cells/mL, counted with the Coulter Counter
- Incubation conditions: incubated for 3 days under continuous light in an incubator shaker apparatus; the temperature at approx. 23 °C
- Test cultures: After the incubation period the cell concentration of the algae reached a sufficient density (approx. 1 167 200 cells/mL), and the pre-culture was diluted to approx.100,000 cells/mL with mineralized water, ready to be used as inoculum. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 23 °C
- pH:
- at the beginning of the test: 8.0 - 8.3
at the end of the test: 8.4 - 8.7 - Nominal and measured concentrations:
- - TOC analysis of the stock solution gave a value of 2.133 mg TOC/L corresponding to 2.603 mg/L test item (further diluted 1: 1 0, 1: 1 00 and 1: 1000 with demineralized water)
- nominal: 2.603, 0.260, 0.026, 0.002 mg/L - Details on test conditions:
- TEST SYSTEM
- Control end cells density: 667 cells/mL x 10E+03
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: Temperature in the incubator was recorded at the beginning and at the end of incubation. pH-value was measured in all test vessels at the beginning and at the end of the test.
OTHER TEST CONDITIONS
- Photoperiod: continuous light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Coulter counter every 24 h
- Microscopic examination: Algae were microscopically examined at the end of exposure. One test vessel of each test concentration group was compared with selected control algae with regard to cell abnormalities. - Reference substance (positive control):
- no
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.26 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: on the basis of TOC analysis
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 2.603 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: saturated solution
- Details on results:
- There was a slight reduction in growth at the 1:100 and 1:10 dilutions. However, the saturated solution did not show growth inhibition. Hence, this slight growth reduction was not considered substance-related but due to biological variability.
- Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test): No relevant observations were made in comparison with the algae of the control group. - Reported statistics and error estimates:
- The values for the average specific growth rate, the area below the growth curves, the percentage inhibition of the cell growth and the reduction in average growth rate were calculated by the computer program "ALG" (version 1.2), Informatik CMF, Schering AG, Berlin, Germany.
- Validity criteria fulfilled:
- yes
- Remarks:
- see "Any other information on results incl. tables"
- Conclusions:
- ErC50 (72 h) > 2.603 mg/L (nominal)
- Executive summary:
The purpose of this study was to determine the effects of Fluocortolon-A-Acetat on the growth of the green algae Desmodesmus subspicatus (former name: Scenedesmus subspicatus). The study was conducted in agreement with the test guideline OECD no. 201 and EC guideline C.3.
Fluocortolon-A-Acetat was incubated with an algae population of Scenedesmus subspicatus for a test duration of approximately 72 hours. For the preparation of the test solutions a suspension with a nominal loading of 102 mg/L test item was stirred for approximately 24 hours. This suspension was filtered and the resulting solution served as the highest concentration (saturated solution). The organic carbon concentration of the stock solution was analysed with a TOC analyzer at the start of the incubation as 2.6 mg/L. It was further diluted 1:10, 1:100 and 1:1000 with demineralized water to give nominal concentrations of 0.26 mg/L, 0.026 mg/L and 0.002 mg/L. The algae were exposed to each concentration in triplicate. Six vessels were prepared for the control. The algae were incubated in an incubator shaker under continuous light and a temperature of approximately 23 °C. The pH-values were measured at the beginning and end of exposure. As a parameter for the growth of the algae population, the cell concentrations of the test and control solutions were counted with an electronic particle counter ("Coulter Counter").
The 72h-EC50 of Fluocortolone-A-Acetat for green algae Desmodesmus subspicatus was higher than a saturated solution (2.6 mg/L). Therefore Fluocortolone-A-acetate is not toxic to the green algae Desmodesmus subspicatus.
This toxicity study is classified as acceptable and satisfies the guideline requirements for the algae study.
Reference
Validity criteria for the measurement of the algae toxicity
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Exponentially growing test organisms are exposed to the test substance in batch cultures over a period of normally 72 hours. | Test organisms: Scenedesmus subspicatus Test period: 72 h | Yes |
The biomass in the control cultures should have increased exponentially by a factor of at least 16 within the 72-hour test period. | Growth in the control flasks after 3 days showed a reproduction rate that was greater than a factor of 16. Areas ("biomass integrals") under the growth curves of Scenedesmus subspicatus also showed a minimum of 16-fold increase after 72 h. | Yes |
For the final definitive test at least five concentrations, arranged in a geometric series with a factor not exceeding 3.2, should be selected. For test substances showing a flat concentration response curve a higher factor may be justified. The concentration series should preferably cover the range causing 5-75 % inhibition of algal growth rate. | Test concentration no.: 5 Factor of geometric series of test conc.: 10
| Yes with restrictions |
The cultures should be maintained at a temperature in the range of 21 to 24°C, controlled at ± 2°C. | Incubation was at 23 ± 0°C | Yes |
The pH of the control medium should not increase by more than 1.5 units during the test. | Solvent control medium pH was 8.0 at the beginning of the test and 8.7 after 72 h. |
Yes
|
Description of key information
A study was conducted in agreement with the test guideline OECD no. 201 and EC guideline C.3 to determine the toxicity of Fluocortolon-A-Acetat towards algae. The 72 h-ErC50 of Fluocortolone-A-Acetat for green algae Desmodesmus subspicatus was higher than a saturated solution (ErC50 > 2.603 mg/L (nominal)).
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 2.603 mg/L
Additional information
"Should read: >2.603 mg/L"
There was a slight reduction in growth at the 1:100 and 1:10 dilutions. However, the saturated solution (2.603 mg/L) did not show growth inhibition. Hence, this slight growth reduction was not considered substance-related but due to biological variability.
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