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EC number: 601-489-9 | CAS number: 1176-81-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Oct - Nov 1998
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Remarks:
- TOC analyzer
- Details on sampling:
- For the concentration analysis samples were taken from each test vessel with the test product at 0 hour and 48 hours.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: 100 mg test item were suspended in 1000 mL tap water under constant stirring for 24 hours. Thereafter, the solution was filtered through a glassfibre filter. The filtered solution was used as test solution.
- Eluate: no
- Differential loading: no
- Controls: yes, test medium control - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Schering AG, Experimental Toxicology, Berlin, Germany
- Age: 24-25 hours
- Holding: Parent animals were kept in glass vessels in water of the same quality and temperature as under experimental conditions. The animals were continuously fed with a laboratory culture of microalgae, e.g. Chlorella vulgaris.
- Water: Normal tap water was used as dilution water, not polluted with harmful concentrations of chlorine, heavy metals or other substances, based on quarterly measurements - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 251 mg/L (CaCO3)
- Test temperature:
- 20.8 - 21.2 °C
- pH:
- control: beginning: 7.7, end: 8.6
saturated solution: beginning: 8.6, end: 9.1 - Dissolved oxygen:
- 7.6 - 9.1 mg/L
- Conductivity:
- 755 µs/sec
- Nominal and measured concentrations:
- nominal: control, 100 mg/L
mean measured concentration: below the detection limit (1 mg/L) - Details on test conditions:
- I. Exposure conditions:
- 20 Daphnia were used for the test concentration and also for the control group, randomly distributed into groups of five
- Labeled 100 mL test vessel were used and filled with 45 mL of the test solution or tap water (control group) and 5 mL of the dilution water with the introduction of the Daphnia
- Experimental design: test was performed with 4 replicates of the test solution and the dilution water control with 5 Daphnia in each vessel
- Method of initiation: neonates were placed in prepared media
- Photoperiod: 12 h light : 12 h dark
- Aeration: none
- Feeding: None
- Criteria of effects: Immobilization and visual abnormalities
II Observations
- Immobilization and visual abnormalities were recorded at 24 and 48 hours
III. Hydrographical measurements:
- One additional vessel (measurement vessel) without Daphnia for each test and control solution, resp. was used for the measurements of pH, temperature and oxygen content
- pH, oxygen concentration and temperature were measured at 0 and at 48 hours - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Remarks:
- see "Any other information on results incl. tables"
- Conclusions:
- EC50 (48 h) > 100 mg/L (nominal)
EC50 (48 h) > 1 mg/L (measured TOC) - Executive summary:
The study was conducted as a limit test in agreement with OECD guideline no. 202 to assess the toxicity of Fluocortolon-A-Acetat towards Daphnia. 20 juvenile Daphnia magna were used for the test solution of Fluocortolon-A-Acetat and 20 Daphnia for the tap water control. The Daphnia were exposed for a period of 48 hours under static conditions. Immobilization was recorded at 24 hours and 48 hours. For the preparation of the test solution, a suspension with a nominal loading of 100 mg/L was stirred for 24 hours. This suspension was filtered through a glassfibre filter. The saturated solution obtained by this procedure was used. The organic carbon concentration of the test solution was analyzed with a TOC analyzer at the start of exposure and 48 hours thereafter. The mean concentration was below the detection limit (1 mg/L). The test temperature was in the range of 20.8 to 21.2 °C, the pH ranged between 7.7 and 8.6, and the oxygen concentration between 7.6 and 9.1 mg/L. The light/dark rhythm was adjusted to 12 hours/12 hours.
A 48h-EC50 > 1 mg/L was determined for Fluocortolon-A-Acetat towards Daphnia. No toxic effect could be determined up to a limit concentration of 100 mg/L (nominal).
This toxicity study is classified as acceptable and satisfies the guideline requirements for the acute daphnia study.
Reference
Validity criteria for the measurement of the daphnia toxicity:
Target condition according to guideline: | Actual condition according to the study: | Validity criteria met: |
Young daphnids, aged less than 24 hours at the start of the test, are exposed to the test substance at a range of concentrations for a period of 48 h. | Age of daphnids used: 24 - 25 h Exposure time: 48 h | Yes |
In the control, including the control containing the solubilising agent, not more than 10 % of the daphnids should have been immobilized. (Not more than 10 % of the control daphnids should show immobilisation or other signs of disease or stress, for example, discoloration or unusual behaviour such as trapping at surface of water) | The control mortalities are below the 10 % value which is regarded as the limit for natural mortality. As the physico-chemical measurements show, the composition of the test water corresponds to the nominal values. The EC50 of the reference substance lies within the required range. Thus, the study conditions correspond to the standard. | Yes |
Test concentrations: At least five test concentrations should be used. They should be arranged in a geometric series with a separation factor preferably not exceeding 2.2. The highest concentration tested should preferably result in 100 % immobilization, and the lowest concentration tested should preferably give no observable effect. | Test concentrations used: 1 (limit tests) Test concentration separation factor: None as limit test
| Yes (with acceptable deviation) |
Description of key information
A study was conducted as a limit test in agreement with OECD guideline no. 202 to assess the toxicity of Fluocortolon-A-Acetat towards Daphnia. A 48h-EC50 > 1 mg/L was determined for Fluocortolon-A-Acetat towards Daphnia under static conditions. No toxic effect could be determined up to a limit concentration of 100 mg/L (nominal).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 1 mg/L
Additional information
"Should read: > 1 mg/L"
The 48 h-EC50 to Daphnia magna is higher than a saturated solution of Fluocortolon-A-Acetat (<1 mg/L), determined by TOC analysis.
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