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EC number: 876-151-9 | CAS number: 2292123-68-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- March 23 2006. Annex 5 corrected, July 28, 2011
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: "Algal Growth Inhibition Test" - Testing method for New Chemical Substances of Japanese Chemical Substances Control Law
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: OECD Guidance Document No. 23
- Version / remarks:
- February 2019
Guidance Document on Aqueous-phase Aquatic Toxicity Testing of Difficult Test Chemicals - GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: single concentration (nominal concentration: 100 mg/L)
- Sampling method: Solution sampled separately from the preparation container (at start of exposure); mixed solution was taken out with equal volumes of the test solution in the test vessels in each test level (at the end of exposure). Algae removed by centrifugation (3000rpm, 10 minutes)
- Sample storage conditions before analysis: samples were analysed straightaway after collection. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: A saturated solution was prepared. 100g test item and 1000mL medium were mixed to produce the test concentration 100 mg/L and stirred by a magnetic stirrer for 48h to prepare the test solution. The test solution was divided into each test vessel.
- Controls: Culture medium (negative control) prepared using the same method as the test solution but no test item added.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): No used; not applicable
- Concentration of vehicle in test medium (stock solution and final test solution(s) or suspension(s) including control(s)): not applicable
- Test concentration separation factor: not applicable (limit test)
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): yes: suspension, precipitation and insoluble matter were observed.
- Other relevant information: undissolved test item had no physical effect on the test organisms - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- TEST ORGANISM
- Common name: Algae
- Strain: ATCC 22662
- Source (laboratory, culture collection): American Type Culture Collection
- Method of cultivation: Passage cultured under sterile conditions in test facility
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions (same as test or not): same as test
- Any deformed or abnormal cells observed: no - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Remarks on exposure duration:
- As recommended in guideline.
- Post exposure observation period:
- Not applicable
- Test temperature:
- 22.3 - 22.8°C
- pH:
- 7.7 - 8.1
- Nominal and measured concentrations:
- Nominal: 100 mg/L
Measured: 0.00102 mg/L - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer flask with gas-permeable Silicosen
- Material, size, headspace, fill volume: Glass, 300 mL, yes, 100mL/vessel
- Aeration: continuous shaking
- Initial cells density: 0.75 x 10E4 cells/mL (CFV = 28)
- Control end cells density: CFV = 4500
- No. of vessels per concentration (replicates): 6
- No. of vessels per control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
OTHER TEST CONDITIONS
- Sterile test conditions: yes
- Adjustment of pH: no
- Photoperiod: continuous illumination
- Light intensity and quality: Nominal 90µmol-m-2.s-1 in wavelength of 400-700nm
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
- Determination of cell concentrations: Spectrophotofluorometer
- Chlorophyll measurement: yes biomass - (CFV)
TEST CONCENTRATIONS
- Spacing factor for test concentrations: Not applicable - limit test
- Justification for using less concentrations than requested by guideline: Not applicable
RANGE FINDING STUDY
- Test concentrations: 100 mg/L (saturated solution)
- Results used to determine the conditions for the definitive study: yes - Reference substance (positive control):
- yes
- Remarks:
- Not conducted in parallel with study but lab conducts positive controls periodically to confirm the reproducibility of the test system. Period of study - March 26 - April 29, 2019 72h ErC50 = 1.1mg/L
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Remarks:
- growth rate
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Remarks on result:
- other: No growth rate inhibition was observed during the test.
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.001 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Remarks on result:
- other: No growth rate inhibition was observed in the study.
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Observation of abnormalities (for algal test):
- Unusual cell shape: no
- Colour differences: no
- Flocculation: no
- Adherence to test vessels: no
- Aggregation of algal cells: no
- Any stimulation of growth found in any treatment: no
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: yes, precipitation was observed
- Effect concentrations exceeding solubility of substance in test medium: no effects observed. - Results with reference substance (positive control):
- 72h ErC50 = 1.1mg/L
- Reported statistics and error estimates:
- See Table 3
- Validity criteria fulfilled:
- yes
- Remarks:
- -Cell growth in controls = Factor 161 (criteria: 16) - Mean coefficient of variation for section-by-section specific growth rates = 20% (criteria <35%) - Coefficient of variation of average specific growth rates in replicate controls = 1.1% (criteria <7%)
- Conclusions:
- No growth inhibition was observed during the limit test. The 72h EC50 was calculated as >0.00102 mg/L greater than the solubility of the test item in the test medium.
Reference
Table 1: Value of biomass at each time
Measured concentration (mg/L) | No. | Chlorophyll fluorescence value (relative unit) | |||
0 hoursa | 24 hours | 48 hours | 72 hours | ||
Control | A | 28 | 210 | 1200 | 5100 |
B | 28 | 210 | 1400 | 5300 | |
C | 28 | 220 | 1300 | 5200 | |
D | 28 | 210 | 1200 | 4900 | |
E | 28 | 210 | 1300 | 5100 | |
F | 28 | 200 | 1300 | 4500b | |
Mean | 28 | 210 | 1300 | 5000 | |
S.D. | 0 | 5.7 | 57 | 280 | |
0.00102 | A | 28 | 200 | 1200 | 5000 |
B | 28 | 200 | 1300 | 4900 | |
C | 28 | 200 | 1300 | 5000 | |
D | 28 | 190 | 1300 | 4900 | |
E | 28 | 200 | 1200 | 5100 | |
F | 28 | 200 | 1300 | 4900 | |
Mean | 28 | 200 | 1300 | 5000 | |
S.D. | 0 | 2.6 | 46 | 100 |
b: The value based on the measured value of pre-culture
c: The minimum cell growth in the control (bioass at the end of exposure / biomass at the start of exposure) 4500/28 = 161
Table 2: Growth rate and growth inhibition rate
Measured concentration (mg/L) | No. | Growth rate (0-3d) | Growth inhibition rate (%) |
Control | A | 1.74 | - |
B | 1.75 | - | |
C | 1.74 | - | |
D | 1.72 | - | |
E | 1.73 | - | |
F | 1.69 | - | |
Mean | 1.73 | - | |
S.D. | 0.0196 | - | |
0.00102 | A | 1.73 | -0.073 |
B | 1.72 | 0.28 | |
C | 1.73 | -0.24 | |
D | 1.72 | 0.47 | |
E | 1.74 | -0.53 | |
F | 1.72 | 0.40 | |
Mean | 1.73 | 0.050 | |
S.D | 0.00686 | 0.40 |
Table 3: Result of statistical analysis
Measured concentration (mg/L) | Statistical analysis | Statistical procedure |
0.00102 | n.a. | F test Aspin-Welch t test |
Table 4: Variation of growth rate in control
<variation for section-by-section specific growth rates in the controls>
Control No. | Mean | Standard deviation | Coefficient of variation (%) | |
A | 1/74 | 0.296 | 17 | 20 (mean) |
B | 1.75 | 0.364 | 21 | |
C | 1.74 | 0.355 | 20 | |
D | 1.72 | 0.334 | 19 | |
E | 1.73 | 0.322 | 19 | |
F | 1.69 | 0.378 | 22 |
<variation of average specific growth rates in replicate controls>
0-3 days | |
Mean | 1.73 |
Standard deviation | 0.0196 |
Coefficient of variation (%) | 1.1 |
Table 5: Measured concentration of the test item in test solution
Nominal concentration (mg/L) | Measured concentration (mg/L) (Percentage of measured concentration versus that at the start %) | ||
At the start | At the end | Time weighted mean | |
Control | <LOQ | - | - |
100 | 0.000113 | 0.00368 | 0.00102 |
LOQ: 0.0000501 mg/L
The time weighted mean is calculated by the following calculation:
[72 (C0-C72)/(lnC0-lnC72]/72
Cn: the measured concentration at n hours
lnCn: the natural logarithm of Cn
Description of key information
In a study conducted in accordance with OECD Guideline 201 on Pseudokirchneriella subcapitata, the 72h ErC50 >100 mg/L (nominal) was reported. Based on measured concentrations, no mortality or abnormal behaviour was observed up to the limit of solubility of the test item (TWA measured with analytical verification at 0.00102 mg/L).
Key value for chemical safety assessment
Additional information
The 72h EC50 value is >100 mg/L it is not possible to report this as the key value for chemical safety assessment in the boxes above.
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