3-acetamido-5-amino-4-hydroxybenzenesulphonic acid

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11.-13.05.1993

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Specific details on test material used for the study:

Code number: FAT - 92'333/A
Batch number: K 37/19.5.92
Purity: 97.2 %
Appearance: solid
Solubility: 1.3 g/l in water
Storage: room temperature
Expiration date: 01.06.93

Reference substance:
Potassium dichromate (EC50: 0.93 mg/l)

Analytical monitoring:

yes

Details on sampling:

The concentration of the test substance at the beginning and at the end of the test was analysed by the Analytical Services K, Textile Dyes Division. All test medium samples for the analytical determinations were taken after mixing of the test solutions.

Vehicle:

no

Details on test solutions:

test article in 250 ml beakers containing 100 - 200 ml
test medium with 10 animals per beaker
The test water medium was prepared with bidistilled water.
The pH is in a range of 7.8 - 8.2
The medium was oxygen saturated at the beginning of the test.

Test organisms (species):

Daphnia magna

Details on test organisms:

Test system / Test organism
The test organism was the species Daphnia magna. The daphnids were bred in the laboratories of the Textile Dyes Division, Product Ecology TF under standardized conditions. For the test, animals with age of less than 24 hours were used. The quality of the daphnids will be checked at least every 3 months by determining the EC50 values (24 h) for potassium dichromate (range: 0,8 -1,5 mg/l). For each concentration, 20 Daphnia magna were used.

Experimental conditions
48 hours at 21 °C, 16 h light / 8 hours dark
The daphnids were exposed to the test article in 250 ml beakers containing 100 - 200 ml test medium with 10 animals per beaker. All tests are running in duplicate. During exposure, the animals were kept at 21 ± 1 °C with 16 hours illumination.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

48 h

Hardness:

Theoretical water hardness : 136 mg/l CaCCO3
Ca / Mg ratio 4:1
Na/K ratio 10:1
alkalinity 0.8 mmol/l

Test temperature:

start 22 °C

pH:

7.7

Dissolved oxygen:

saturated

Details on test conditions:

End: Nominal cone, (mg/l) 58; 100; 180; Blank
Oxygen (%) 97; 95; 93; 99;
pH 7.8; 7.5; 7.7; 8.1;
Temperature (°C) 20.6; 20.6; 20.6; 20.6;

The test medium used had the following composition:
stock solution concentration:
NaHCOs: 65 mg/l
MgS04 * 7H2O: 127.5mg/l
KCL: 5.9 mg/l
CaCl2 * 2 H2O: 294
SeO2: 1.4 µg/l
NaBr: 16.1µg/l
Vitamine B 12: 1.0 µg/l

The test water medium was prepared with bidistilled water.
The pH is in a range of 7.8 - 8.2
The medium was oxygen saturated at the beginning of the test.
Theoretical water hardness: 136mg/l CaCOs
Ca / Mg ratio: 4: 1
Na / K ratio: 10:1
alkalinity: 0.8 mmol/l

Reference substance (positive control):

yes

Remarks:

potassium dichromate, EC50=0.93 mg/L

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

134.8 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Oxygen concentration and pH

The oxygen concentration and the pH was controlled at the beginning and at the end of the test.

Start:

Oxygen: - saturated

pH: 7.7 (stock solution]

Temperature: 22°C

 

End:

Nominal conc, (mg/l)	58	100	180	Blank
Oxygen (%)	97	95	93	99
pH	7.8	7.5	7.7	8.1
Temperature (°C)	20.6	20.6	20.6	20.6

 

Immobilization of Daphnids

The results of the study are given in table 1.

 

Table 1

Conc, of Test substance   (mg/l actual)	No. of animals	No. of responses 24 h	No. of responses 48 h	% immobility 24 h	% immobility 48 h
Blank	10 10	0 0	0 0	0	0
42.5	10 10	0 0	0 1	0	5
79.9	10 10	0 0	1 3	0	20
134.2	10 10	0 0	5 5	0	50

Calculation of EC - values

Influence of FAT-92'33/A on Daphnia magna after 48 hours of exposure. In the freshly prepared test medium 78.7 - 87.2 % of the nominal test concentrations was analytically determined. After 48 hours 67.2 - 72.5 % of the nominal test concentrations was found in the test medium. In the stock solution and in all test concentrations the test substance was dissolved without any sedimentation or flocculation. Since the analyzed concentrations were clearly lower than the nominal concentrations the mean values were calculated between the measured concentrations of the start and the end of the test. The EC - values reported are related to the mean measured concentrations of the test

substance. In the control no immobilization of the daphnids was observed after 48 hours. The EC0 value could not be calculated, but is lower than 42.5 mg/l. The EC50 value was calculated to be 134.8 mg/l.

The EC values are evaluated as follows: (mg/l)

 

	EC0	EC 50	EC100
after 24 h	> 134.2	> 134.2	> 134.2
after 48 h	<42.5	134.8*	> 134.2

*The EC50 values are calculated with the LOGIT MODEL.

95 % confidence limit (48 h): 104.8 - 285.9

 

Validity criteria fulfilled:

yes

Remarks:

not toxic to daphnia

Conclusions:

FAT - 92'333/A is considered to be practically non toxic to daphnids.

Executive summary:

The acute toxicity of test substance to Daphnia magna was determined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The test was performed in compliance with the Good Laboratory Practice Regulations. The nominal concentrations tested were 58, 100 and 180 mg test article/l, and a control without any additions. In the freshly prepared test medium 78.7 - 87.2 % of the nominal test concentrations was analytically determined. After 48 hours 67.2 - 72.5 % of the nominal test concentrations was found in the test medium. In the stock solution and in all test concentrations the test substance was dissolved without any sedimentation or flocculation. Since the analyzed concentrations were clearly lower than the nominal concentrations the mean values were calculated between the measured concentrations of the start and the end of the test.The EC-values reported are related to the mean measured concentrations of the test substance. In the control no immobilization of the daphnids was observed after 48 hours. The EC0 value could not be calculated, but is lower than 42.5 mg/l.

The EC50 value was calculated to be 134.8 mg/l.

Results:

EC0: <42.5 mg/L

EC50: 134.8 mg/L

EC100: >134.2 mg/L

Conclusion : FAT - 92'333/A is practically non toxic to Daphnia magna.

Description of key information

The acute toxicity of FAT - 92'333/A to Daphnia magna was detennined in a 48-hour static test according to the OECD Guideline for Testing of Chemicals, Section 2, No. 202, adapted 4 April 84, and EEC Directive 84/449, L 251, Part C 2. The EC50 was determined to 134.8 mg/L which means that the test item is practically non-toxic to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

134.8 mg/L