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Diss Factsheets
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EC number: 429-080-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16th June 1999 - 29 September 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.3 (Algal Inhibition test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 429-080-9
- EC Name:
- -
- Cas Number:
- 198904-85-7
- Molecular formula:
- C17 H21 N3 O2
- IUPAC Name:
- Tert-butyl 2-[4-(2-pyridinyl) phenylmethyl] hydrazine carboxylate
- Test material form:
- solid: bulk
Constituent 1
- Specific details on test material used for the study:
- Identity of test material same as for substance defined in section 1, white powder with 98.4% purity
Sampling and analysis
- Analytical monitoring:
- yes
Test solutions
- Vehicle:
- no
- Details on test solutions:
- Identity and concentration of auxiliary solvent for dispersal: No auxiliary solvent was required.
Test organisms
- Test organisms (species):
- Selenastrum sp.
- Details on test organisms:
- Selenastrum capricornutum (new name: Pseudokirchnerella subcapitata), strain CCAP 278/4
Study design
- Test type:
- static
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Nominal and measured concentrations:
- The measured concentrations of BMS 233110-01 in the test media were determined at the start and end of the exposure period. The mean measured concentrations in the 0.32, 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments were 0.28, 0.52, 1.05, 1.98, 4.15 and 8.24 mg/L respectively, representing 88, 83, 84, 79, 83 and 82% of nominal exposure concentrations. Therefore the toxicity of BMS 233110-01 was based on the mean measured exposure concentrations.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.3 mg/L
- Basis for effect:
- biomass
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.68 mg/L
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.98 mg/L
- Basis for effect:
- growth rate
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1.98 mg/L
- Basis for effect:
- biomass
- Details on results:
- The inhibition of growth of S. capricornutum exposed to BMS 233110-01 was based on mean measured exposure concentrations
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the average specific growth rate, the 0-72hr ErC50 of BMS233110-01 to S.Capricornutum was calculated to be 5.68 mg/l. Based on the area under the growth curve, the 0-72hr EbC50 of BMS233110-01 to S.Capricornutum was calculated to be 4.30 mg/l.
Based on both the average specific growth rate and the area under the growth curve, the highest no observed effect concentrations (NOECs) of BMS233110-01 to S.Capricornutum were calculated to be 1.98mg/l. - Executive summary:
The nominal concentration range used in the definitive test was 0.32, 0.63, 1.25, 2.5, 5.0 and 10 mg/L. The range of concentrations was based on the results of a rangefinding
test, which is not reported. A 10 mg/L stock solution was prepared by direct addition of BMS 233110-01 to nutrient medium. The stock solution was used to provide the 10 mg/L treatment and further diluted with nutrient medium to prepare the following nominal concentrations: 0.32, 0.63, 1.25, 2.5 and 5.0 mg/L. A control treatment was prepared by the addition of nutrient medium only to the test vessels.
The measured concentrations ofBMS 233110-01 in the test media were determined at the start and end of the exposure period. The mean measured concentrations in the 0.32, 0.63, 1.25, 2.5, 5.0 and 10 mg/L treatments were 0.28, 0.52, 1.05, 1.98, 4.15 and 8.24 mg/L respectively, representing 88, 83, 84, 79, 83 and 82% of nominal exposure concentrations. Therefore the toxicity of BMS 233110-01 was based on the mean measured exposure concentrations.
Based on the average specific growth rate (μ) the 0 to 72 hours ErCso of BMS 233110-01 to S. capricornutum was calculated to be 5.68 mg/L (95% confidence limits could not be calculated). Based on the area under the growth curves , the 0 to 72 h EbCso of BMS 233110-01 to S. capricornutum was calculated to be 4.30 mg/L (95% confidence limits could not be calculated).
The highest no observed effect concentrations (NOEC's) of BMS 233110-01 to S. capricornutum were calculated to be 1.98 mg/L.
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