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EC number: 203-339-4 | CAS number: 105-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 29 Sep - 27 Oct 2000
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Version / remarks:
- adopted 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Niedersächsisches Umweltministerium, Hannover, Germany
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: Municipal sewage treatment plant, Hildesheim, Germany
- Preparation of inoculum for exposure: The activated sludge was filtered with folded filter. The first 200 mL of the filtrate are not used. The second filtrate effluent from the domestic waste water is used to initiate inoculation.
- Concentration of sludge: 10 E+04 to 10 E+06 cfu/L per test vessel
- Initial cell/biomass concentration: 0.2 ml of the inoculum was given in each BOD bottle - Duration of test (contact time):
- 28 d
- Initial conc.:
- 3 mg/L
- Based on:
- test mat.
- Initial conc.:
- 7.29 mg/L
- Based on:
- ThOD
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to the guideline 301 D
- Test temperature: 20 °C
- pH: 7.39 (stock solution)
- Aeration of dilution water: One day before the test started the demineralized water for the test medium was aerated until oxygen saturation and then left at room temperature for at least 20 h.
- Other: Detailes of parameters of the test media are listed in Table 1 and 2 in the section "Any other information on material and methods incl. tables".
TEST SYSTEM
- Culturing apparatus: BOD bottles with glass stoppers (300 mL)
- Number of culture flasks/concentration: 5 x 2 per test item
- Method used to create aerobic conditions: One day before the test started the demineralized water for the test medium was aerated until oxygen saturation and then left at room temperature for at least 20 h. The stock solutions were filled to the BOD bottles with a siphon. The incubation vessels were closed without air bladders.
- Measuring equipment: respirometer recording O2 consumption
- Test performed in closed vessels due to significant volatility of test substance: closed system
SAMPLING
- Sampling frequency: 0, 7, 14, 21 and 28 days
- Sampling method: oxygen concentration was measured
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, 2 bottles per sampling
- Toxicity control: yes, 2 bottles per sampling
- Other: Reference control: yes, 2 bottles per sampling
- Abiotic sterile control: no
- Reference substance:
- acetic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 79
- Sampling time:
- 28 d
- Details on results:
- The biodegradation came to 73% after 14 days and to a maximum of 81% after 21 days. At this point of time the plateau was reached. Since the 10-day window was met the substance is considered to be readily biodegradable according to the OECD criteria. Results are reported in detail in Table 1 to 3 in "Any other information on results incl. tables".
- Results with reference substance:
- The reference control reached the pass level > 60% after 5 days. After 14 days a biodegradation rate of 96% was reached.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The biodegradation came to 73% after 14 days and to a maximum of 81% after 21 days. At this point of time the plateau was reached. After 28 d the biodegradation was 79%. Since the 10-day window was met the substance is considered to be readily biodegradable according to the OECD criteria.
Reference
Validity criteria:
The percentage degradation of the functional control reached the pass level of > 60% after 14 days. The oxygen depletion in the inoculum control did not exceed 1.5 mg dissolved oxygen/L after 28 d. The depletion came to a maximum of 1.60 mg O2/L after 21 days. This has no impact an integrity and quality of the study. The residual concentration of oxygen in the test bottles did not fall below 0.5 mg/L. The residual concentration of oxygen in the functional control bottles was less than 0.5 mg O2/L after 14 days. The residual concentration of oxygen in one toxicity control bottle was less than 0.5 mg O2/L after 21 days. The difference of extremes of replicate values of removal of the test item at the end of the test was less than 20%.
Table 1: Biodegradation of the test item in comparison to the reference control and the toxicity control
|
biodegradation [%] study day [d] |
|||
7 |
14 |
21 |
28 |
|
Test Item 3 mg/L |
70 |
73 |
81 |
79 |
Reference Control 10 mg/L |
88 |
96 |
94 |
94 |
Toxicity Control 1.5 + 5 mg/L |
84 |
88 |
92 |
92 |
Table 2: Oxygen determination of the inoculum control and functional control
Study day |
inoculum control |
functional control 10 mg/L ThOD 0.78 mg O2/mg |
||||||||
[d] |
O2 [ mg/L] |
O2 [mg/L] |
BOD |
degr. |
||||||
|
C1 |
C2 |
mv |
O2-depl. |
R1 |
R2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
0 |
9.04 |
8.98 |
9.01 |
- |
9.00 |
8.94 |
8.97 |
- |
- |
- |
7 |
8.12 |
7.94 |
8.03 |
0.98 |
1.09 |
1.19 |
1.14 |
6.85 |
0.69 |
88 |
14 |
7.79 |
7.73 |
7.76 |
1.25 |
0.23 |
0.20 |
0.22 |
7.50 |
0.75 |
96 |
21 |
6.86 |
7.95 |
7.41 |
1.60 |
0.12 |
0.10 |
0.11 |
7.26 |
0.73 |
94 |
28 |
7.59 |
7.74 |
7.67 |
1.34 |
0.31 |
0.45 |
0.38 |
7.25 |
0.73 |
94 |
Table 3: Oxygen determination of the test item and the toxicity control
Study day |
test item 3 mg/L |
toxicity control 1.5 mg/L test item + 5 mg/L reference item |
||||||||||
|
ThOD 2.43 mg/L |
ThOD 1.16 mg O2/mg |
||||||||||
[d] |
O2 [ mg/L] |
BOD |
Degr. [%] |
O2 [ mg/L] |
BOD |
Degr. [%] |
||||||
|
P1 |
P2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
T1 |
T2 |
mv |
[mg O2/L] |
[mg O2/mg] |
[%] |
0 |
8.99 |
8.90 |
8.95 |
- |
- |
- |
8.95 |
9.04 |
9.00 |
- |
- |
- |
7 |
2.87 |
2.93 |
2.90 |
5.07 |
1.69 |
70 |
1.78 |
1.62 |
1.70 |
6.32 |
0.97 |
84 |
14 |
2.20 |
2.49 |
2.35 |
5.35 |
1.78 |
73 |
1.12 |
1.11 |
1.12 |
6.63 |
1.02 |
88 |
21 |
1.19 |
1.74 |
1.47 |
5.88 |
1.96 |
81 |
0.56 |
0.30 |
0.43 |
6.97 |
1.07 |
92 |
28 |
1.90 |
1.84 |
1.87 |
5.74 |
1.91 |
79 |
011 |
0.65 |
0.68 |
6.98 |
1.07 |
92 |
mv = mean value BOD = biological oxygen demand
degr. = degradation
O2-depl. = Oxygen depletion
ThOD= Theoretical oxygen demand
Description of key information
Readily biodegradable: 79% degradation after 28 d, OECD 301D
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
Additional information
One experimental study is available investigating the biodegradability of the substance. The study was performed according to OECD 301 D (GLP). Non-adapted, domestic activated sludge from a sewage treatment plant was used as inoculum. The test item was found to be readily biodegradable under the test conditions. The biodegradation came to 73% after 14 days and to a maximum of 81% after 21 days. After 28 d the biodegradation was 79%. At this point of time the plateau was reached. The pass level for ready biodegradability was reached after 7 days of exposure, therefore the test substance is considered to be “readily biodegradable” according to the OECD criteria. A toxicity control containing both, reference substance and test substance, did not indicate inhibitory effects to the inoculum (73% degradation after 14 d). All validity criteria of the study were met.
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