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EC number: 258-110-1 | CAS number: 52697-38-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
- Principles of method if other than guideline:
- The cutaneous penetration kinetics of Disperse Blue 79:1 in human abdominal skin samples were measured using in vitro techniques for a 6 h period along with parallel testing of other subtances. The amount of test substance penetrating skin samples and collected in the effluent were measured by liquid scintillation spectrometry or high-performance liquid chromatography (HPLC).
Test material
- Reference substance name:
- 2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate
- EC Number:
- 222-813-1
- EC Name:
- 2,2'-[[5-acetamido-4-[(2-bromo-4,6-dinitrophenyl)azo]-2-methoxyphenyl]imino]diethyl diacetate
- Cas Number:
- 3618-72-2
- Molecular formula:
- C23H25BrN6O10
- IUPAC Name:
- 2-{[2-(acetyloxy)ethyl]({4-[ (E)-2-(2-bromo-4,6-dinitrophenyl)diazen-1-yl]-5acetamido-2-methoxyphenyl})amino}ethyl acetate
- Details on test material:
- Disperse Blue 79:1 (Br)
CAS No. 3618-72-2
Constituent 1
- Radiolabelling:
- yes
Results and discussion
- Total recovery:
- No information available.
Any other information on results incl. tables
Skin penetration of the test substance, applied to human abdominal skin, could not be detected by HPLC above the minimum detection limit of 1 µg/mL. The results thus indicate that human dermal exposure to the test substance would probably not result in a significant absorbed dose.
Applicant's summary and conclusion
- Conclusions:
- Skin penetration of Disperse Blue 79:1 (Br), applied to human abdominal skin, could not be detected by HPLC above the minimum detection limit of 1 µg/mL. The results thus indicate that human dermal exposure to the test substance would probably not result in a significant absorbed dose.
- Executive summary:
The cutaneous penetration kinetics of 3H-water (550 µl) or 14C-mannitol (550 µl; 1 mg/ml) through excised pig ear and human abdominal skin samples were measured using in vitro techniques for a 6-hr period. In addition, the cutaneous penetration kinetics of 14C-Disperse Blue 79:1 (Br) (110 µl; 5 mg/ml), Reactive Blue 19 (550 µl; 1 mg/ml) and Direct Blue 218 (550 µl; 1 mg/ml) were evaluated by the same in-vitro techniques using human abdominal skin samples. The CAS numbers were 2580-78-1 (Reactive Blue 19), 10401-50-0 (Direct Blue 218), 3618-72-2 (Dispersion Blue 79:1 (Br)), and 69-65-8 (D-Mannitol). Test substances penetrating skin samples and collected in the effluent were measured by liquid scintillation spectrometry or high-performance liquid chromatography (HPLC).
For pig ear skin, the lag time before skin penetration of 14C-mannitol or 3H-water reached a steady-state rate was 2.77 and 1.75 hr, respectively. The steady-state rate of penetration of mannitol or water through pig ear skin was calculated to be 0.2 µg/cm2/hr and 6.12 mg/cm2/hr, respectively, while the Kp value was calculated to be 0.21 x 10-3cm/hr and 6.12 x 10-3cm/hr, respectively. These results were similar to those reported by Scott and Dick (1990). For human abdominal skin, the lag time before skin penetration of 14C-mannitol or 3H-water reached a steady-state rate was 3.49 and 2.17 hr, respectively. The steady-state rate of penetration of mannitol or water through human skin was calculated to be 0.006 µg/cm2/hr and 2.43 mg/cm2/hr, respectively, while the Kp value was calculated to be 0.006 x 10-3cm/hr and 2.43 x 10-3cm/hr, respectively.
These results show that significantly more 3H-water can penetrate both pig ear skin and human abdominal skin than 14C-mannitol. However, the total penetration of water and mannitol after 6 hr of exposure was 2.7-times and 55-times less for human skin than for pig ear skin. Similarly, both the steady-state penetration rates and permeability constants for water and mannitol were approximately 2.5-times and 30-times less, respectively, for human skin than for pig ear skin. These results would suggest that pig ear skin is not a good animal model for human cutaneous penetration of chemicals.
For the 14C-DB-79:1 applied to human abdominal skin, the lag time before skin penetration reached a steady-state rate was 3.38 hr. The steady-state rate of penetration of DB-79:1 through human skin was calculated to be 0.0057 µg/cm2/hr. The Kp value for the skin penetration of this dye, however, could not be calculated. Skin penetration of the Reactive Blue 19 and Direct Blue 218 applied to human abdominal skin could not be detected by HPLC above the minimum detection limit of 1 µg/ml.
Little or no Disperse Blue 79:1 (Br), Reactive Blue 19 or Direct Blue 218 was detected penetrating human skin samples. These results indicate that human dermal exposure to any of these 3 dye compounds would probably not result in a significant absorbed dose.
(Sun, 1995).
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