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EC number: 204-246-1 | CAS number: 118-33-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
Experimental study for target was conducted by R. Jung, D.et al (Food and chemical toxicology, 1992) to determine the mutagenic nature. Mutagenicity of substance Naphthalene-1,3-disulfonic acid, 6-amino- was assessed by using Ames test. It shows negative mutagenic result. The substance Naphthalene-1,3-disulfonic acid, 6-amino-is considered to be not mutagenic in Salmonella typhimurium. Hence it cannot be classified as gene mutant in vitro.
Supported by an experimental study for target ,which was conducted by European Chemicals Bureau (IUCLID dataset, European Chemicals Bureau, 2000) to determine the mutagenic nature. Mutagenicity of substance 6-aminonaphthalene-1,3-disulfonic acid was assessed by using Ames test. The test material was exposed to Salmonella typhimurium strain TA 1535, TA 100, TA 1537, TA 98in the presence and absence of metabolic activation. It shows negative mutagenic result. The substance6-aminonaphthalene-1,3-disulfonic acid is considered to be not mutagenic in Salmonella typhimurium strain TA 1535, TA 100, TA 1537, TA 98.Hence it cannot be classified as gene mutant in vitro.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Justification for type of information:
- Data is from publication.
- Qualifier:
- according to guideline
- Guideline:
- other: As mention below
- Principles of method if other than guideline:
- Mutagenicity of substance Naphthalene-1, 3-disulfonic acid, 6-amino- was assessed by using Ames test.
- GLP compliance:
- not specified
- Type of assay:
- bacterial reverse mutation assay
- Specific details on test material used for the study:
- - Name of test material:6 amino-1,3 naphthalene –disulfonic acid
- Molecular formula: C10H9NO6S2
- Molecular weight: 303.314 g/mole
- Smiles notation: c12c(cc(S(O)(=O)=O)cc1S(O)(=O)=O)cc(N)cc2
- InChl: 1S/C10H9NO6S2/c11-7-1-2-9-6(3-7)4-8(18(12,13)14)5-10(9)19(15,16)17/h1-5H,11H2,(H,12,13,14)(H,15,16,17)
- Substance type: Organic
- Physical state: Solid - Species / strain / cell type:
- S. typhimurium, other:
- Details on mammalian cell type (if applicable):
- Not applicable.
- Additional strain / cell type characteristics:
- not specified
- Cytokinesis block (if used):
- Not specified.
- Metabolic activation:
- not specified
- Test concentrations with justification for top dose:
- Not specified.
- Vehicle / solvent:
- Not specified.
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- not specified
- True negative controls:
- not specified
- Positive controls:
- not specified
- Details on test system and experimental conditions:
- Not specified.
- Rationale for test conditions:
- Not specified.
- Evaluation criteria:
- Not specified.
- Statistics:
- Not specified.
- Key result
- Species / strain:
- S. typhimurium, other: Not specified
- Metabolic activation:
- not specified
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- not specified
- Untreated negative controls validity:
- not specified
- Positive controls validity:
- not specified
- Conclusions:
- The substance Naphthalene-1,3-disulfonic acid, 6-amino- is considered to be not mutagenic in Salmonella typhimurium.
- Executive summary:
Mutagenicity of substance Naphthalene-1,3-disulfonic acid, 6-amino- was assessed by using Ames test.It shows negative mutagenic result. The substance Naphthalene-1,3-disulfonic acid, 6-amino-is considered to be not mutagenic in Salmonella typhimurium. Hence it can not be classified as gene mutant in vitro.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Genotoxicity In-vitro
Various publications were reviewed to determine the mutagenic nature of 6-aminonaphthalene-1,3-disulfonic acid (118-33-2). The studies are as mentioned below:
Experimental study for target was conducted by R. Jung, D.et al (Food and chemical toxicology, 1992) to determine the mutagenic nature. Mutagenicity of substance Naphthalene-1,3-disulfonic acid, 6-amino- was assessed by using Ames test. It shows negative mutagenic result. The substance Naphthalene-1,3-disulfonic acid, 6-amino-is considered to be not mutagenic in Salmonella typhimurium. Hence it cannot be classified as gene mutant in vitro.
Supported by an experimental study for target ,which was conducted by European Chemicals Bureau (IUCLID dataset, European Chemicals Bureau, 2000) to determine the mutagenic nature. Mutagenicity of substance 6-aminonaphthalene-1,3-disulfonic acid was assessed by using Ames test. The test material was exposed to Salmonella typhimurium strain TA 1535, TA 100, TA 1537, TA 98in the presence and absence of metabolic activation. It shows negative mutagenic result. The substance6-aminonaphthalene-1,3-disulfonic acid is considered to be not mutagenic in Salmonella typhimurium strain TA 1535, TA 100, TA 1537, TA 98.Hence it cannot be classified as gene mutant in vitro.
In a study for structurally and functionally similar read across chemical, Gene mutation toxicity study was performed by F. RAFII et al.( Food and Chemical Toxicology,1997) to determine the mutagenic nature of D&C Red No. 33 (RA CAS No3567-66-6; IUPAC name: disodium 5-amino-4-hydroxy-3-(phenyldiazenyl)naphthalene-2,7-disulfonate. The read across substances share high similarity in structure and log kow .Therefore, it is acceptable to derive information on mutation from the analogue substance. In a gene toxicity test, Salmonella typhimurium Strain-TA 98, TA 100 were exposed to D&C Red No. 33 in the concentration of 50 and 200 µg/plate with and without metabolic activation. In addition D&C Red No. 33 metabolites were also prepared by treating with azo reductase -producing bacteria namely Clostridium strain isolated from the human gastrointestinal tract. The results showed that there was no evidence of gene toxicity after treatment with D&C Red No. 33 in the concentration of 50 and 200 µg/plate in Salmonella typhimurium Strain-TA 98, TA 100. Independently of tested D&C Red No. 33 reduced metabolite in the concentration of 50 and 200 µg/plate showed that there was no evidence of gene toxicity. Therefore, it is considered that D&C Red No. 33 and its reduced metabolites in the concentration of 50 and 200 µg/plate do not cause genetic mutation(s) when Salmonella typhimurium Strain-TA 98, TA 100 exposed to the test chemical in the presence and absence of metabolic activation (S9).
In a study for structurally and functionally similar read across chemical, Gene mutation toxicity study was performed by National Institute of Technology and Evaluation (Japan chemicals collaborative knowledge database, 2017) to determine the mutagenic nature of Sodium 4-aminonaphthalene-1-sulfonate (130-13-2). The read across substances share high similarity in structure and log kow .Therefore, it is acceptable to derive information on mutation from the analogue substance. Gene toxicity in vitro study was observed for Sodium 4-aminonaphthalene-1-sulfonate (130-13-2) in Salmonella typhimurium TA100, TA1535, TA98, TA1537, Escherichia coli WP2 uvrA. The test substance was observed at the concentration of 313, 625, 1250, 2500, 5000 µg/plate in the presence and absence of metabolic activation. The test result was considered to be negative with and without metabolic activation. Therefore Sodium 4-aminonaphthalene-1-sulfonate was considered to be non mutant in vitro. Hence cannot be classified as genetox in vitro.
Based on the data available for the target chemical as well as its read across substance and applying weight of evidence 6-aminonaphthalene-1,3-disulfonic acid (118-33-2)does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
Justification for classification or non-classification
Thus based on above annotation and CLP criteria available for the target chemical and its read across substance 6-aminonaphthalene-1,3-disulfonic acid (118-33-2)does not exhibit gene mutation in vitro. Hence the test chemical is not likely to classify as a gene mutant in vitro.
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