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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1987
Report date:
1987

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Biochemical Oxygen Demand (1981) Method for the Examination of Waters and Associated Materials; Department of the Environment UK Standing Committee of Analysts (1981)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
O-ethylhydroxylamine
EC Number:
402-030-3
EC Name:
O-ethylhydroxylamine
Cas Number:
624-86-2
Molecular formula:
C2 H7 N O
IUPAC Name:
O-ethylhydroxylamine
Details on test material:
- Name of test material (as cited in study report): Substance H109360 & Q125
- Physical state: colourless liquid
- Analytical purity: 50%
- Lot No.: EFI-100

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Activdted sludge was obtained from a sewage treatment works which treats sewage of primarily domestic origin.
- Laboratory culture: activated sludge was kept aerated at 20 °C and the pH was maintained at 7 ± 0.2
- Preparation of inoculum for exposure: One week prior to the test the activated sludge was washed by centrifuging, resuspended in the test medium and the solids concentration determined . Twelve units were set up containing the test medium which had been inoculated as a master batch with activated sludge (A/S) at a concentration of 33 .4 mg A/S/L . These units were loosely capped and allowed to stir for one week.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
8 mg/L
Based on:
other: test solution (test substance + 50% water)
Initial conc.:
20 mg/L
Based on:
other: test solution (test substance + 50% water)
Initial conc.:
4 mg/L
Based on:
test mat.
Initial conc.:
10 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
O2 consumption
Details on study design:
TEST SYSTEM
The samples were incubated in a completely full and stoppered bottle using a dilution medium consisting of distilled water containing inorganic salts and a bacterial inoculum prepared from a treated domestic sewage effluent.
The BOD was calculated by measuring the dissolved oxygen in the test bottles (3/test level and measurement day) in comparison with the controls (3/measurement day).

SAMPLING
- Sampling frequency: at day 5, 10, 15, 20, 25 and 28
- Temperature: 20 °C

Results and discussion

% Degradation
Parameter:
% degradation (O2 consumption)
Value:
64
Sampling time:
28 d
Details on results:
- Lag phase (4 mg/L test substance): 5-10 days
- Lag phase (10 mg/L test substance). 10-15 days
- The longer lag phase at the test concentration of 10 mg/L indicates the probability that the test substance is inhibítory to aerobic biodegradation at high test concentrations.

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable