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EC number: 258-649-2 | CAS number: 53585-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- from 1990-02-14 to 1990-06-21
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- EU Method C.8 (Toxicity for Earthworms: Artificial Soil Test)
- Principles of method if other than guideline:
- Toxizitaetstest fuer Regenwuermer nach 88/302 EWG
- GLP compliance:
- yes
- Specific details on test material used for the study:
- The purity of the material used for the test was lower than the purity of the actual material. It contained ca. 26% of isomers of dimethyl tribenzenes. However, based on comparable basic structure of the impurities and the assumption that any toxic effects would increase with higher molecular weight and lipophilicity, we regard that the studies conducted with the lower purity material being "worst case" and can be used for the evaluation of the actual product.
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on preparation and application of test substrate:
- - Method of mixing into soil: The test substance was mixed with silica sand and afterwards added to soil substrate in a plastic bag. Water was added to a theoretical water content of approx. 40% and the substrate was thoroughly mixed.
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: Eisenia GmbH&Co. Produktions- und Vertrieb KG, Kapellenstraße 25, Wiesbaden, Germany
- Age at test initiation: at least 2 months
- Weight at test initiation: 300-432 mg
ACCLIMATION
- Acclimation period: not mentioned - Study type:
- laboratory study
- Substrate type:
- other: mixture of peat and horse dung (1:1)
- Limit test:
- yes
- Total exposure duration:
- 14 d
- Post exposure observation period:
- no
- Test temperature:
- not mentioned
- pH:
- 7.4
- Moisture:
- 42.9 - 45.6 %
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): Makrolon cages with perforated plexiglass covers, size not mentioned
- Amount of soil or substrate: not mentioned
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
- No. of replicates per vehicle control: not applicable
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): mixture of peat and horse dung (1:1)
- Organic carbon (%): not mentioned
- Maximum water holding capacity (in % dry weigth): not mentioned
- CEC: not mentioned
- Pretreatment of soil: not mentioned
- Storage (condition, duration): fresh prepared
- Stability and homogeneity of test material in the medium: not mentioned
VEHICLE CONTROL PERFORMED: yes
TEST CONCENTRATIONS
- Range finding study: not performed - Nominal and measured concentrations:
- nominal concentration, not measured: 300 / 550 / 1000 mg/kg dry soil
- Reference substance (positive control):
- yes
- Remarks:
- chloracetamide, historical data
- Key result
- Duration:
- 7 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 850 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Details on results:
- - Mortality at end of exposure period: 0/40 (0%) all concentrations after 7 days; 300 mg/kg: 0/40 (0%), 550 mg/kg 3/40 (7.5%), 1000 mg/kg 29/40 (72.5%) after 14 days
- Total mass of adults at beginning of test: 300 mg/kg = 324 mg; 550 mg/kg = 321 mg; 1000 mg/kg = 320 mg (mean)
- Changes in body weigth of live adults (% of initial weight) at end of exposure period: not mentioned
- No. of offspring produced: not mentioned
- No. of unhatched cocoons: not mentioned
- Morphological abnormalities: not mentioned
- Behavioural abnormalities: not mentioned
- Other biological observations: not mentioned - Results with reference substance (positive control):
- RESULTS: TEST WITH REFERENCE SUBSTANCE (historical data, 1990-05-11)
- Concentrations: 0, 10, 30 and 100 mg 2-chloroacetamide/kg dry soil
- Results (mortality):
after 7 days: 0 mg/kg: 0/20 (0%); 10 mg/kg: 0/20 (0%) after 7 days; 30 mg/kg: 0/20 (0%) 100 mg/kg: 0/20 (100%)
after 14 days: 0 mg/kg: 1/20 (5); 10 mg/kg: 2/20 (10%) after 7 days; 30 mg/kg: 0/20 (0%) 100 mg/kg: 0/20 (100%) - Reported statistics and error estimates:
- no statistics performed
- Validity criteria fulfilled:
- yes
- Conclusions:
- LC 50 (14d) = 850 mg/kg dry soil.
- Executive summary:
The acute toxicity of Marlotherm S to the earthworm eisenia foetida was determined during a 14 day exposure period according to EEC Methods for Determination of Ecotoxicity, Directive 88/302/EEC. Three concentrations 300, 550 and 1000 mg/kg dry soil, the test substance was mixed with silica sand and afterwards added to soil substrate. After 7 and 14 days the effects of the test substance on the earthworms' survival were examined. The 7 day LC 50 is > 1000 mg/kg.
The 14 day LC50 of Marlotherm S to Eisenia foetida was observed to be 850 mg/kg dry soil.
Reference
RESULTS: EXPOSED
- Nominal concentrations: 300 / 550 / 1000 mg/kg dry soil
- Effect data (Mortality): 0 / 0 / 0% after 7 days; 0 / 7.5 / 72.5% after 14 days
RESULTS: CONTROL
- Effect data (Mortality): 0/40 (0%) after 7 and 14 days
Description of key information
The LC 50 value derived after 14 days of exposure is equal to 850 mg/kg dry soil.
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil macroorganisms:
- 850 mg/kg soil dw
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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