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EC number: 258-649-2 | CAS number: 53585-53-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: According to OSPAR draft protocol for HOCNF, in compliance with GLP requirements
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Version / remarks:
- draft protocol at the time of testing
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- Duplicate aliquots (4 ml) of solution or algal cell suspension from the range finding and definitive tests were mixed with Quickszint 1 scintillation fluid (Zinsser Analytic) prior to determination of radioactive content by liquid scintillation counting as a gel. Duplicate aliquots (up to 1 ml) of other liquid samples were mixed with Quickszint 1 scintillation fluid prior to determination of radioactive content by liquid scintillation counting as a liquid.
- Vehicle:
- no
- Details on test solutions:
- Appropriate amounts of radiolabelled and non-radiolabelled DBT were combined in methanol. The solutions were transferred into 1 litre capacity glass flasks and methanol removed under a stream of gaseous nitrogen. Each flask was amended with 1 litre algal medium and subjected to ultrasonication (20 min) followed by continuous stirring (18-24 h). For the range finding and limit tests, stock suspensions were prepared by adding 1 litre of algal medium to 45.4 µg [14C]-DBT and subjecting the mixture to ultrasonication (20 min) followed by continuous stirring (18-24 h). The suspension was centrifuged (1000 r.p.m., 30 min) using a Jouan Model C412 bench centrifuge and total radioactivity measured in the supernatant fraction. In the range finding test, the supernatant fraction was diluted as required with algal growth medium to produce the lower test concentrations.
- Test organisms (species):
- Skeletonema costatum
- Details on test organisms:
- The marine diatom Skeletonema costatum (Chrysophyta) was used for these studies. Starter cultures (Strain 1077/3) were obtained from the Culture Collection of Algae and Protozoa (CCAP), Oban, Scotland.
Transfers of the algae were made regularly into fresh algal growth medium to provide suitable axenic subcultures which were in the exponential phase of growth, for test inoculations. - Test type:
- static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no
- Hardness:
- not relevant (marine water)
- Test temperature:
- 21.2 ± 1°C throughout the test period
- pH:
- 7.97-8.45 during the test
- Dissolved oxygen:
- no data
- Salinity:
- Algal growth medium (Guillard's Medium - Appendix 2) was prepared by adding specific weights/volumes of nutrient enrichments to 0.2 µm filtered natural seawater. The seawater was collected from a site on the east coast of Scotland and was free from known sources of pollution. The seawater typically has a salinity of 32 ± 2% and a pH of 8.0 ± 0.3.
- Nominal and measured concentrations:
- For range finding test, nominal concentrations : 17.2, 3.5, 0.9 and 0.2 µg equiv/l-1.
For limit test, initial measured concentration was 16 µg/L. After 72h exposure, concentrations measured in the 6 test flasks ranged from 4.2 to 6.0 µg/L with an average of 5.3 µg/L - Details on test conditions:
- For the range finding test, test concentrations were prepared by dilution of the supernatant fraction with algal medium. The test was conducted, over a 72 h period, using the following series of measured concentrations of test material in algal growth medium, inoculated with 10E3-10E4 cells/ml Skeletonema costatum:
17.2, 3.5, 0.9 and 0.2 µg equiv/L
A control was also set up using algal growth medium alone.
A further control was set up using algal growth medium alone using flasks which had not been treated with Sigmacote®.
Two flasks were prepared at each concentration.
A further single flask was prepared at each concentration which was not inoculated with algae, to provide information of the bio-availability of the test material.
Each flask contained 50 ml of test solution.
Based on the results from the range finding test, a limit test was conducted at the maximum solubility [14C]-DBT.
For the limit test, 10 flasks containing test solution at maximum solubility prepared as described above and 6 flasks containing algal growth medium alone were tested. The concentration of radioactivity in the supernatant fraction was measured as 16.0 µg equiv/L. An additional 3 control flasks which had not been treated with Sigmacote® were set up using algal growth medium alone.
Each flask contained 50 ml of test solution.
The inoculum volume was calculated to yield 10E3-10E4 cells/mL Skeletonema costatum.
Two further single flasks containing test solution or algal growth medium alone were set-up and not inoculated with algae to provide information on the bioavailability of the test material.
Algal cell concentrations were measured in the inoculum and in each test flask at 72 h after the commencement of the range finding test. Algal cell concentrations were measured in the inoculum and in each algal flask at 24, 48 and 72 h after the commencement of the limit test. Aliquots were removed by pipette and cell concentrations determined using a Compound Light Microscope and Improved Neubauer Counting Chambers. Between 4 and 6 cell counts were made from each flask at each timepoint.
Duplicate aliquots were removed from test solutions at the commencement of each test and from the contents of each flask at the end of each test for determination of test material concentration by analysis of total radioactivity.
The pH was measured at the start and finish of the definitive test. Environmental chamber temperature fluctuations were monitored daily. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Key result
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 9.23 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No effect observed at the highest tested concentration
- Key result
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 9.23 µg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: No effect observed at the highest tested concentration
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- > 16 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 16 µg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Details on results:
- The deviation between µ(ave) gave results over 0-72 h for the cultures incubated with [14C]-DBT at its maximum solubility and the corresponding controls was very small (<2%). This achieved statistical significance, a result which is judged to reflect low variability between replicates within these treatment groups. No other statistically significant differences were found and it was concluded that the No Observed Effect Concentration (NOEC) and the EC50 value exceed the measured values found in the limit test.
These results obtained during the definitive test are detailed in the "table 6 and 7" in the section "attached background material".
Measured concentrations of total radioactivity are given in the "table 8" in the section "attached background material". The maximum solubility achieved was 16.0 µg equiv.l-1. Algal cultures incubated in this solution contained 5.3 µg equiv.l-1 at the end and a flask containing the same solution without algae contained 1.1 µg equiv.l-1. These changes confirm that [14C]-DBT is bioavailable and also became bound to the test vessels during the test. No radioactivity was detected in control flasks throughout the test. - Results with reference substance (positive control):
- In accordance with UK MAFF Guidelines (1993), a reference toxicant (3,5-dichlorophenol at 1.5 µg equiv/L) was included as part of the study. Three flasks containing reference toxicant, inoculated with the same concentration of Skeletonema costatum and maintained under the same conditions as the test flasks, showed a 62% inhibition of growth over the 72 h test period. As this fell within the acceptance criteria of 20-80% inhibition as specified by UK MAFF, the test is considered to have been valid.
- Validity criteria fulfilled:
- yes
- Remarks:
- Validity criteria of the ISO 10253 are fullfiled: Control density increased by a factor > 16 in 72 h (106). Variation coefficient of the control specific growth rate > 7% ( 1,35%). pH not increased by more than 1 during the test.
- Conclusions:
- No EC50 or NOEC could be determined as no inhibition of growth was observed at highest soluble concentration (16 µg/L).
- Executive summary:
Study of inhibition of growth of the marine algae Skeletonema costatum was carried out according to the protocol described in OSPAR document (now ISO 10253 standard). No significant inhibition was observed at the highest soluble (measured) concentration, which was 16.0 µg/L
Reference
Description of key information
No growth inhibition of algae is observed at the highest soluble concentration.
Key value for chemical safety assessment
Additional information
Several studies are available but for 4 out of 5 there is uncertainty on exposure concentration and/or use of vehicle in conditions where interference cannot be excluded. Only one study fulfills confidence criteria. It makes use of marine unicellular algae, but their conclusions will also be applied to freshwater, as indicated in the Guidance. This study (Inveresk, 1997) has been carried out on Skeletonema costatum, use was made of a 14C labelled test substance sample that allowed to have precise measurement of exposure concentrations. At the highest soluble concentration of 16 µg/L (initial measured concentration) corresponding to 9.2 µg/L (geometric mean measured concentration), there was no difference of growth with the controls. This study is considered to be reliable and the key study.
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