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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GPL guideline study without restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
2-Methoxy-6-methylbenzoic acid
EC Number:
612-182-4
Cas Number:
6161-65-5
Molecular formula:
C9H10O3
IUPAC Name:
2-Methoxy-6-methylbenzoic acid
Details on test material:
- Name of test material (as cited in study report): BAS 650 F - Benzoe-(Säure)
- Physical state: solid, crystalline
- Analytical purity: 98.4 %
- Lot/batch No.: 30802/128
- Storage condition of test material: at room temperature

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: stock solution of 100.2 mg/L in Media

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: water flea
- Source: The clone of Daphnia magna STRAUS 182
- Age at study initiation (mean and range, SD): < 24 hours (at least 3rd brood progeny)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Details on test conditions:
TEST SYSTEM
- Test vessel: Test tubes (glass) with fiat bottom (nominal volume 20 ml)
- Testl volume: 10 mL
- Aeration: none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark
- Light intensity: About 1 - 8 pE/(m² s) at a wave length of 400 - 750 nm
Reference substance (positive control):
yes
Remarks:
potasssium dichromate

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Since the analytically determined concentrations of the test substance in the test solutions were within +20% of the nominal concentrations, the effect concentration can be expressed relative to the nominal concentration.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes