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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2003
Report date:
2003

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-Methoxy-6-methylbenzoic acid
EC Number:
612-182-4
Cas Number:
6161-65-5
Molecular formula:
C9H10O3
IUPAC Name:
2-Methoxy-6-methylbenzoic acid
Details on test material:
- Name of test material (as cited in study report): BAS 650 F - Benzoe-(Säure)
- Physical state: solid, crystalline
- Analytical purity: 98.4 %
- Lot/batch No.: 30802/128
- Storage condition of test material: at room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
- Source of inoculum/activated sludge: Laboratory wastewater plants treating municipal and synthetic sewage
Duration of test (contact time):
21 d
Initial test substance concentrationopen allclose all
Initial conc.:
31.2 mg/L
Based on:
test mat.
Initial conc.:
5 mg/L
Based on:
DOC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
TEST CONDITIONS
- Test temperature: 22 ± 2 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Test performed in open system: no

SAMPLING
- Sampling frequency: regularly intervals

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control
Reference substance
Reference substance:
aniline

Results and discussion

% Degradation
Parameter:
% degradation (DOC removal)
Value:
90
Sampling time:
21 d

BOD5 / COD results

Results with reference substance:
90 - 100% after 14 days,

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable