Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-661-5 | CAS number: 109-28-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Additional information
For justification of read-across please see separate document attached to section 13 of the IU5 dossier.
Short-term toxicity to fish:
The test substance was tested in an acute toxicity test with
Brachydanio rerio (zebra fish) under semi-static conditions in
accordance with EEC method C.1 and OECD Guideline 203. Zebra fish were
exposed to 0.1, 0.18, 0.32, 0.58 and 1.05 mg test item/L. No chemical
analysis of the test concentrations were performed during the test and
all concentrations mentioned are nominal concentrations. The LC50 (96h)
was calculated to be 0.22 mg/L with 95% confidence limits of 0.19 and
0.26 mg/L. The highest concentration causing no mortality (no observed
effect concentration, NOEC) after 96 hours amounted to 0.10 mg/L,
whereas 100% mortality was produced within 30 hours at 0.32 mg/L. At the
concentrations of 0.32, 0.58 and 1.05 mg/L the fish showed reduced
activity before their death.
Short-term toxicity to aquatic invertebrates:
A toxicity study on aquatic invertebrates with
N-[3-(Dimethylamino)propyl]oleamide is not available. Consequently,
read-across was applied using a characteristically similar compound,
N-[3 -(dimethylamino)propyl].
The acute toxicity of the test item to Daphnia magna was assessed
according to the method C2 of the European Directive 92/69/CEE and the
guideline 202 (part I) of the OECD. Daphnia were exposed in a static
test to 0.016, 0.031, 0.063, 0.125, 0.25, 0.5 and 1 mg test item /L.The
test was performed with 5 Daphnia per vessel. Testing flasks were
incubated in darkness at 19.2± 0.5 °C for 48 hours. For each exposure
concentration, the percentage of immobilisation after 24 hours and 48
hours was recorded. As no analytical method was available, effective
concentrations have been calculated using the initial nominal
concentrations of the test substance. The calculated EC50 after 24 hours
and 48 hours were 0.58 (95% CI: 0.38 -1.1) mg/L and 0.28 (95% CL: not
determined) mg/L respectively. The appearance of the test solutions was
visually checked at the beginning and at the end of the test. Solutions
were found to be clear over the period of the test. No precipitation was
observed at the end of the test.
Long-term toxicity to aquatic invertebrates:
A reproduction study on aquatic invertebrates with
N-[3-(Dimethylamino)propyl]oleamide is not available. Consequently,
read-across was applied using a characteristically similar compound,
N-[3 -(dimethylamino)propyl].
In oder to assess the toxicity of the test substance in an aquatic
environment, a Daphnia magna reproduction test under semi static
conditions was conducted in accordance with OECD Guideline No. 211 and
EU method C.20. Some modifications to the guideline were applied. The
test was carried out according to the bulk approach using enriched
natural surface water with a low Dissolved Organic Carbon (DOC) and
Total Suspended Solids (TSS) content, allowing a more environmentally
realistic determination of the effects of the test chemical to be made.
Young Daphnia, aged less than 24 hours, were exposed to the nominal
concentrations of 0.004, 0.015, 0.048, 0.15 and 0.5 mg test item/L over
a time period of 21 days. Primary test criterion of toxicity used was
reproductive capacity, expressed as the total number of neonates per
adult daphnid alive at the end of the test. Other endpoints based on
adult mortality, length and weight were calculated if possible.
Analytical determinations of the test solutions showed that
approximately 80% of the concentrations could be detected in the stock
solution and in the highest 3 concentrations. The recovery in the lower
concentrations fell below 80%. Loss of the chemical at lower
concentrations was considered a characteristic of the test chemical as
was accepted as part of the bulk approach. It was concluded that the
test organisms were fully exposed to the test chemical in keeping with
the bulk approach and the nominal concentrations were used for
calculations of the effect levels.
Based on the reproduction and length data of the surviving daphnia a
toxic effect can be determined. The NOEC value and LOEC value, based on
reproduction and adult length were 0.048 mg/L and 0.15 mg/L
respectively. The EC10 based on reproduction was calculated as 0.07 mg/L
confidence limits could not be calculated. The EC10 based on length was
calculated as 0.14 mg/L confidence limits could not be calculated. The
dry weight endpoints was considered unreliable due to the inaccuracy
involved in weighing the low number of surviving daphnids. The adult
mortality endpoint was not considered reliable due to physical effects
being the most likely cause of the effects.
Toxicity to aquatic algae and cyanobacteria:
A toxicity study on aquatic algae with N-[3-(Dimethyl
lamino)propyl]oleamide is not available. Consequently, read-across was
applied using a characteristically similar compound.
The chronic toxicity of the test item to fresh-water green algae
Pseudokirchneriella subcapitata was determined according to the method
C.3 of the Directive 92/69/EEC of the European Commission, which is in
conformity with the OECD Guideline 201. Algae were exposed to a range of
concentrations of the test item dissolved in dilution water. The toxic
effect measured during the assay was the inhibition of cellular
multiplication over a time period of 72 hours. The study was performed
using 100 mL glass erlenmeyer flasks stoppered bungs of cellulose,
containing 50 mL of test solution inoculated with an algal suspension so
that the initial cell concentration was equal to 1 x 10^4 cells/mL. Test
flasks were incubated at 23 ± 1 °C continuously shaken and constantly
illuminated. The cell density was measured daily. Analytical chemistry
and physico-chemical measurements were carried out at the beginning and
the end of the test. After 72 hours an EC50 value of 0.003 mg/L based on
cell growth and 0.005 mg/L based on growth rate was determined. The NOEC
was 0.002 and 0.003 mg/L based on cell growth and growth rate,
respectively. The very low EC50 values may be related to a true
algicidal effect of the test item. Effective concentrations and NOEC
have been calculated using the geometric average values between initial
and final concentrations of the test substance. Concentrations were
measured by liquid chromatography coupled with mass spectrometry.
Initial and final concentrations were not equivalent to nominal
concentrations.
Toxicity to microorganisms:
In an activated sludge respiration inhibition test the influence of
the test item on the activated sludge by measuring the respiration rate
was evaluated according to OECD guideline 209, corresponding to EU C.11.
Four experiments were carried out. Activated sludge from a domestic
sewage treatment plant was used as inoculum in all tests.
3,5-Dichlorophenole was used as reference item in the experiments.
Fist, a limit-test using the nominal concentration 1000 mg/L was
performed. After three hours a mean respiration inhibition of 90% of the
activated sludge was determined. Next, a pre-test was performed likewise
using three concentrations ranging from 1 to 100 mg/L. The treatments
showed no inhibition compared with the controls. Then a main study was
performed using five concentrations in duplicate, ranging from 1000 to
100 mg/L (nominal). The dry matter of the activated sludge was
determined as 3.88 g suspended solids/L, giving a concentration of 1.54
g suspended solids/L in the test. The test item showed
concentration-related inhibition of the activated sludge down to a
concentration of 320 mg/L, no inhibition was found in the treatments
containing 180 mg/L. In one replicate of the treatment 100 mg/L, though,
an inhibition value of 21% was determined, which was believed to be an
outlier. Therefore, a verification experiment was performed, using the
concentration 100 mg/L only. The dry matter of the activated sludge was
determined as 4.18 g suspended solids/L, giving a concentration of 1.67
g suspended solids/L in the test. The test item showed no inhibition at
the tested concentration; therefore, the measured value in the main
study had been an outlier. The following results for the test item were
calculated using the inhibition values which were found in the main
study (excluding the outlier):
3 h NOEC = 180 mg/L
3 h EC20 = 280 mg//L (95% confid. interv.: 200 - 350 mg/L)
3 h EC50 = 480 mg/L (95% confid. interv.: 400 - 600 mg/L)
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.