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Diss Factsheets
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EC number: 210-323-0 | CAS number: 612-83-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
Description of key information
3,3´-dichlorobenzidine is not readily biodegradable. Inidications for inherent biodegradability might be artifacts caused by the extraordinary strong adsorption of the substance to organic matter and the spectroscopic mode of analytical substance determination.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
Additional information
In the key study, 3,3´-dichlorobenzidine was tested for "ready biodegradablilty" in a sound and sufficiently well documented study (relibility score 2, reliable with restrictions) according to the OECD-guideline 301 A (AFNOR method) with deviations especially regarding substance concentration (much lower, because of the poor water solubility) and analytics (spectroscopic monitoring of substance concentration). The study results, constituted by the single results of serveral different laboratories taking part, clearly demonstrate that 3,3´-dichlororbenzidine is not readily biodegradable under the applied conditions (0% degradation at the end of the observation period).
Results showing a certain degree of decrease in spectral absorbance under modified experimental conditions (presence of yeast extract as a nutriant source) are interpreated by the authors as inherent biodegradability. However, as demonstrated by radioactive labelling in another published study (Appleton), this decrease in spectroscopic absorption is most probably caused by the strong adsorption of 3,3´-dichlorobenzidine to organic matter. As organic matter is increasing rapidly especially in presence of yeast extract as external carbon source, an ever increasing fraction of test substance is adsorbed to organic matter and thus pelleted before spectroscopic detection.
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