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Diss Factsheets
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EC number: 203-699-2 | CAS number: 109-73-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 24 - 26 June 1994
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods
- Qualifier:
- according to guideline
- Guideline:
- other: Standard guide for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians (ASTM - 1993)
- Qualifier:
- according to guideline
- Guideline:
- other: Methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms (US EPA - 1991)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not relevant - Analytical monitoring:
- yes
- Details on sampling:
- During the definitive exposure period, duplicate water samples were removed from each treatment level and the control at 0, 24 and 48 hours
for analysis of substance concentration.
Duplicate samples of the freshly prepared solutions were removed from an additional replicate vessel of each concentration and control
at 0 and 24 hours of exposure.
Duplicate aged solution samples of each concentration and control were removed from a composite of replicates at test termination or
when 100 % mortality was observed.
Each exposure solution sample was collected from the approximate midpoint of the test vessel with a volumetric pipet. - Vehicle:
- no
- Test organisms (species):
- Ceriodaphnia dubia
- Details on test organisms:
- Age: <= 24hours
Source: Springborn Laboratories culture facility
Feeding during test: combination of a unicellular green algae (Selenastrum capricornutum YCT mixture) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- Moderately hard reconstituted water (96 mg/L as CaCO3)
- Test temperature:
- 24 to 26 °C
- pH:
- 7.8
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations are based on acute daphnia test results.
Nominal concentrations: 1.7 - 3.3 - 6.5 - 13 - 26 mg/L
Measured concentrations: 1.5 - 2.8 - 5.7 - 12 - 22 mg/L
Control: <0.28 mg/L - Details on test conditions:
- TEST SYSTEM
- Material: 40 ml glass vial each containig 40 mL of test solution and sealed with a Teflon lined screw top cap
- Renewal rate of test solution: 24 hours renewal period
OTHER TEST CONDITIONS
- Photoperiod: 16 hours of light and 8 hours of darkness
- Light intensity: 50 to 100 footcandles at the surface of the culture solutions
WATER PARAMETERS
- Source/preparation of dilution water: same source as the water used in the daphnid cultures
- Pesticides: no
- Alkalinity: 68 mg/L
- Conductivity: 370 µmhos/cm
- Total organic carbon: ranged from 0.43 to 0.82 mg/L for the months of december 1993 to may 1994 - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.2 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 5.7 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- - Mortality (%): 0, 0, 0, 100, 100
- Other effects: lethargic at nominal 13 mg/L
- Mortality of control: 0 %
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no
Validity criteria:
1) In the control not more than 10 per cent of the daphnids have been immobilised;
2) Dissolved oxygen concentration at the end of the test has not been lower than 3 mg/L in control and test vessels.
3) Test substance concentration was sufficiently kept. - Results with reference substance (positive control):
- no data
- Validity criteria fulfilled:
- yes
- Conclusions:
- 48h-LC50 (Ceriodaphnia dubia, immobilisation; semi-static) = 8.2 mg/L (mean measured)
- Executive summary:
Mobility inhibition of Ceriodaphnia dubia has been investigated according to U.S. methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms (US EPA - 1991) / U.S. standard guide for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians (ASTM - 1993) and in compliance with GLP.
Applying a semistatic test design (medium change after 24 hr), Ceriodaphnia dubia were exposed for 48 h to an aqueous solution of monobutylamine at nominal concentrations of 1.7 - 3.3 - 6.5 - 13 - 26 mg A.I./L (measured concentrations of 1.5 - 2.8 - 5.7 - 12 - 22 mg A.I./L). The 48h-LC50 was determined at 8.2 mg/L. The No observed acute effects level (NOAEC) is of 5.7 mg/L.
The validity criteria were fulfilled and the study was considered reliable and adequate for the environmental hazard assessment.
Reference
Description of key information
48h-LC50 (Ceriodaphnia dubia, immobilisation) = 8.2 mg/L (mean measured concentration, U.S. guideline ASTM - 1991/1993, 1994)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 8.2 mg/L
Additional information
A reliable key study is available investigating mobility inhibition of Ceriodaphnia dubia according to U.S. methods for measuring the acute toxicity of effluents and receiving waters to freshwater and marine organisms (US EPA - 1991) / U.S. standard guide for conducting acute toxicity tests with fishes, macroinvertebrates and amphibians (ASTM - 1993) and in compliance with GLP (RL1, 1994).
Ceriodaphnia dubia were exposed (semistatic test design, change of media after 24 h) for 48 h to an aqueous solution of monobutylamine at nominal concentrations of 1.7 - 3.3 - 6.5 - 13 - 26 mg A.I./L (measured concentrations of 1.5 - 2.8 - 5.7 - 12 - 22 mg A.I./L). The 48h-LC50 was determined at 8.2 mg/L (mean measured concentration). The No observed acute effects level (NOAEC) is of 5.7 mg/L.
The validity criteria were fulfilled and the study was considered reliable and adequate for the environmental hazard assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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