3,4-dichlorobut-1-ene

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: cytogenicity / chromosome aberration

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Original reference not translated. However, according to OECD SIDS a reliability of 2 was given.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

The influence of 1,4-dichlorbutene and 3,4-dichlorbutene on the crhomosoms of albino rats

Author:

Nalbandyan, T.I. and Gizhlaryan, M.S. : Zh. Eksp. Klin. Med.|25, 335-339 (1985)

Year:

1985

Bibliographic source:

Zh. Eksp. Klin. Med. 25, p 335-339

Reference 2

Reference Type:

secondary source

Title:

SIDS Initial Assessment Report for 11th SIAM

Author:

OECD SIDS

Year:

2003

Bibliographic source:

UNEP Publications

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The possible mutagene activity of 3,4-dichlorbutene was investigated at different exposures time at low dose levels.

GLP compliance:

not specified

Type of assay:

chromosome aberration assay

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichlorobut-1-ene

EC Number:

212-079-0

EC Name:

3,4-dichlorobut-1-ene

Cas Number:

760-23-6

Molecular formula:

C4H6Cl2

IUPAC Name:

3,4-dichlorobut-1-ene

Details on test material:

- Name of test material (as cited in study report): 3,4-Dichlorobut-1-ene
- Analytical purity: 99.2%

Test animals

Species:

rat

Strain:

other: Albino

Sex:

not specified

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: No data available
- Weight at study initiation: 140 – 180 g

Administration / exposure

Route of administration:

inhalation

Details on exposure:

no data

Duration of treatment / exposure:

1, 30, and 120 days

Frequency of treatment:

Once during the 24 hours exposure; 4hrs/day, 5days/week during 30 and 120 days.

Post exposure period:

not reported

Doses / concentrations

Remarks:

Doses / Concentrations:
13.7 or 81.3 mg/m³
Basis:

No. of animals per sex per dose:

36 animals/group (no information about the sex of the animals)

Control animals:

yes

Positive control(s):

no data

Examinations

Tissues and cell types examined:

bone marrow

Evaluation criteria:

50 well-spread and complete metaphase cells per animal were analysed. Aberrations were classified as general method.

Statistics:

No data

Results and discussion

Test results

Sex:

not specified

Genotoxicity:

positive

Toxicity:

not specified

Vehicle controls validity:

not specified

Negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

The concentration of 13.7 and 81.3 mg/m³ caused chromosome damages, mainly of the chromatid type.
The number of aberrations increased in the increasing of concentration and exposure period.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): positive

Executive summary:

Nalbandyan (1985)

The possible mutagen activity of 3,4-dichlorbutene was investigated at different exposures time (1, 30 and 120 day per 5 days/week) at low dose levels (13.7 and 81.3 mg/m³). 3,4-DCB induced chromosomal aberrations in rat bone marrow cells after inhalation exposure for 30 and 120 days (4 hours/day, 5 days/week). The concentration of 13.7 and 81.3 mg/m³ caused chromosome damages, mainly of the chromatid type.