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EC number: 428-100-3 | CAS number: 94239-04-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Bioaccumulation: aquatic / sediment
Administrative data
Link to relevant study record(s)
- Endpoint:
- bioaccumulation in aquatic species: fish
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 305 B (Bioaccumulation: Semi-static Fish Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: Guidelines for the Testing of Chemical SEPA, P.R. China No. 305B "Bioaccumulation: Semi-static Fish Test "(May 2004).
- Deviations:
- no
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Substance ID: F6TF
Lot #: CZ0904060
Purity: 99.56% - Details on sampling:
- Seven fish samples and test solution samples were taken after 22, 23, 24, 25, 26, 27 and 28 days.
Each sample consists of 15 fish and about 500 mL test solution.
The concentrations of the test substance were analysed on the same day or frozen if they were not to be analysed immediately.
Sample Preparation
Water samples were filtered through 0.45 μm membranes and analysed by HPLC. Fish samples were mashed and 1.0 g were weighed and extracted with 2.0 mL of acetonitrile and water (70:30 v/v) by shaking (200 rpm, 0°C) for 10 min. After waiting for 60 min samples were centrifuged (10000 rpm, 0°C) for 10 min and filtered. The temperature was kept at 0°C during operations. The extraction solutions were filtered through 0.45 μm membranes. pH, temperature and dissolved oxygen were recorded at the same time. - Vehicle:
- yes
- Details on preparation of test solutions, spiked fish food or sediment:
- Stock Solutions: The 200 mL stock solution (about 100.0 mg/mL) was prepared by dissolving the substance in ethanol for 7 days.
Test Solutions: The volumes of stock solution added to the 100 L dilution water were 10.0 mL and 1.0 mL for the 10 mg/L and 1 mg/L levels, respectively. After addition of the stock solution, the mixture was stirred by hand for about 5 min. In order to minimise loss of test substance due to evaporation, the test containers were equipped with a tightly fixed covers. - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- Individual total lengths were between 2.5 and 3.5 mm
Total wet weights between 0.2 and 0.4 g - Route of exposure:
- aqueous
- Test type:
- semi-static
- Water / sediment media type:
- natural water: freshwater
- Total exposure / uptake duration:
- 28 d
- Hardness:
- not reported
- Test temperature:
- 23±2 ºC
- pH:
- 6.82 - 7.56
- Dissolved oxygen:
- 6.79 - 6.92 mg/L
- Salinity:
- freshwater
- Details on test conditions:
- Preliminary Acute Toxicity Study
Semi-static method was used to determine the LC50 for zebra fish. The LC50 was more than 100 mg/L (nominal concentration, limited test, 96 hour).
TEST SYSTEM
- Test vessel: 120 L
- Fill volume: 100 L
- Type: closed
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): 24 hours
- No. of organisms per vessel: 120
- No. of vessels per concentration (replicates): 2
- No. of vessels per control / vehicle control (replicates): 1 - Nominal and measured concentrations:
- Nominal concentration: 1.0, 10.0 mg/L
Mean measured concentration at Day 28: 0.976, 4.622 mg/L - Key result
- Type:
- BCF
- Value:
- < 0.06 dimensionless
- Remarks on result:
- other: Conc.in environment / dose:1.0 mg/L and 10.0 mg/L
- Details on kinetic parameters:
- Bioconcentration factors were calculated from the measured concentration in fish and test solution at steady-state using:
BCF28d = Cf/Cw
Cf is concentration in fish at day 28 (mg/kg)
Cw is concentration in water at day 28 (mg/L) - Details on results:
- Preliminary Acute Toxicity Study:
Semi-static method was used to determine the LC50 for zebra fish. The LC50 was more than 100 mg/L (nominal concentration, limited test, 96 hour).
Validity of the Study and Analytical Methods:
The analytical methods were satisfactory.
Recovery of Test Substance in Fish:
The fish analytical method mean recoveries were 98.7±3.1% at 0.5 mg/kg and 74.4±6.1% at 1.26 mg/kg.
Calibration Curve:
Seven solution concentrations were used; 0.10, 0.50, 1.00, 5.04, 12.6, 25.2 and 50.4 μg/mL.
Typical linear regression: y = 12826x; R2 = 0.9997 (x= concentrations; y= peak area).
The limit of detection (LOD) of the method was 0.25 ng. The limit of quantitation (LOQ) was 0.025 mg/L in water and 0.05 mg/kg in fish.
Stability of Test Substance Solution after 24 hrs:
The exchange rate was less than 16.5% after 24 hours.
pH, Dissolved Oxygen and Temperature were maintained within acceptable ranges. Specifically, the concentration of dissolved oxygen was more than 5 mg/L during the exposure period of 28 days.
Results and Conclusions:
Neither mortality nor other adverse effects/diseases were observed in either the control or treated vessels. - Validity criteria fulfilled:
- yes
- Conclusions:
- The BCF in zebra fish was less than 0.06. The test substance has low bioconcentration potential in zebra fish during the exposure period of 28 days.
- Executive summary:
The bioconcentration test was performed with zebra fish exposed to target concentrations of 1.0 mg/L and 10.0 mg/L (nominal) with renewal of test solution every 24 hours for 28 days according to OECD Guideline 305B (Bioaccumulation: Semi-static Fish Test). No detectable concentrations of test substance were found in any sample of fish tissue. The BCF in zebra fish was less than 0.06. The test substance has low bioconcentration potential in zebra fish during the exposure period of 28 days.
Reference
Description of key information
BCF < 0.06 (dimensionless)
Key value for chemical safety assessment
Additional information
The bioconcentration test was performed with zebra fish exposed to target concentrations of 1.0 mg/L and 10.0 mg/L (nominal) with renewal of test solution every 24 hours for 28 days according to OECD Guideline 305B (Bioaccumulation: Semi-static Fish Test). No detectable concentrations of test substance were found in any sample of fish tissue. The BCF in zebra fish was less than 0.06. The test substance has low bioconcentration potential in zebra fish during the exposure period of 28 days.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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