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EC number: 254-879-2 | CAS number: 40306-75-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid
- EC Number:
- 254-879-2
- EC Name:
- 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid
- Cas Number:
- 40306-75-0
- Molecular formula:
- C8H10N2O5S
- IUPAC Name:
- 3-acetamido-5-amino-4-hydroxybenzenesulfonic acid
- Test material form:
- solid: crystalline
- Details on test material:
- - Name of test material : 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid
- Molecular formula : C8H10N2O5S
- Molecular weight : 246.24
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- Age : 10 to 12 weeks
Sex : female
Body weight range : 1.80kg ±200g
Acclimatization : The rabbit was acclimatized to standard laboratory condition for 24 hours in experimental room before study.
No. of animals: Three
Test system
- Vehicle:
- water
- Controls:
- yes, concurrent vehicle
- Amount / concentration applied:
- A dose of 0.1gm of test substance was applied into the one eye of the rabbits. The other eye, which remains untreated, serves as a control.
- Duration of treatment / exposure:
- 21 days
- Observation period (in vivo):
- The eyes were examined at 1, 24, 48 and 72 hours after test substance application.
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- The test compound 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid in the amount of 0.1 gm moistened with distilled water and then placed in the conjunctival sac of one eye of each animal after gentle pulling the lower eye lid away from the eye ball. The eye lids were then gently held together for about one second to prevent the loss of the material. The other eyes which remains untreated, served as control.The eyes of the test animal did not washed for at least 24 hours following instillation of the test compound.
Results and discussion
In vivo
Results
- Irritation parameter:
- overall irritation score
- Basis:
- mean
- Time point:
- other: 21 days
- Score:
- 0.1
- Max. score:
- 0.1
- Reversibility:
- fully reversible
- Irritant / corrosive response data:
- The test compound 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid applied to the eye of New Zealand white rabbit at the dose level of 0.1 gm produced some blood vessels of conjunctiva were observed hyperemic at 1 hours after the application of test substance.
- Other effects:
- However, some blood vessels observed hyperemic upto 24 hours after the application of test compound. There were no other signs observed throughout the observation period of 21 days.
Any other information on results incl. tables
CORNEA
A.Opacity: degree of density (readings should be taken from most dense area)*
OBSERVATION |
SCORE |
No ulceration or opacity |
0 |
Scattered or diffuse areas of opacity (other than slight dulling of normal lustre); details of iris clearly visible |
1 |
Easily discernible translucent area; details of iris slightly obscured |
2 |
Nacrous area; no details of iris visible; size of pupil barely discernible |
3 |
Opaque cornea; iris not discernible through the opacity |
4 |
Applicant's summary and conclusion
- Interpretation of results:
- not irritating
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- From the result obtained from present study, it can be concluded that the test compound 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid is non-irritant to the eyes of New Zealand white rabbit under test condition.
- Executive summary:
The test compound 3-acetamido-5-amino-4-hydroxybenzenesulphonic acid, when applied to conjunctival sac of rabbit in the amount of0.1 gmdid not produces any eye irritation or inflammation. Furthermore, no other clinical signs were recorded after application of test compound such as cage side activity, pain etc. In the confirmatory test, the test compound 3-acetamido-5-amino-4-hydroxy benzene sulphonic acid when applied to the conjunctival sac of the rabbits did not produce eye irritation during the entire observation period. However, some blood vessels observed hyperemic upto 24 hours after the application of test compound. There were no other signs observed throughout the observation period of 21 days.
Based on above findings, it can be concluded that the test compound is practically non-irritant at the tested dose level.
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