Soybean oil, reaction products with ethaneperoxoic acid

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 January - 5 March 2010

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP/guideline study. Limited information provided.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ASTM E1720 Ready/Ultimate Biodegradability

Deviations:

not specified

Principles of method if other than guideline:

Comparable to OECD 301B

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Soybean oil, reaction products with ethaneperoxoic acid

Cas Number:

693217-63-9

Molecular formula:

C59 H106 O11

IUPAC Name:

Soybean oil, reaction products with ethaneperoxoic acid

Test material form:

not specified

Details on test material:

Agrol® CAS# 693217-63-9 No additional information available.

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
Not applicable.

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

sewage, domestic (adaptation not specified)

Details on inoculum:

The inoculum source is the Escatawpa, MS municipal wastewater treatment facility.

Duration of test (contact time):

28 d

Details on study design:

Test bottles were setup in triplicate for statistical validation of results, and a total of 9 sampling events were executed. The inoculum was preconditioned in the test medium via method guidance to remove organic carbon and inorganic carbon prior to testing.

Results and discussion

% Degradation

% Degradation 1

Parameter:

% degradation (CO2 evolution)

Value:

66.72

St. dev.:

0.296

Sampling time:

3 d

% Degradation 2

Parameter:

% degradation (CO2 evolution)

Value:

82.1

St. dev.:

1.142

Sampling time:

7 d

% Degradation 3

Parameter:

% degradation (CO2 evolution)

Value:

89.17

St. dev.:

6.071

Sampling time:

10 d

% Degradation 4

Parameter:

% degradation (CO2 evolution)

Value:

91.96

St. dev.:

1.663

Sampling time:

28 d

Details on results:

Based on the testing conducted in accordance with the specified method above, test product Agrol® achieved 91.96% biodegradation.

BOD5 / COD results

Results with reference substance:

Average CO2 production for the reference substance, dextrose, was 57.17, 82.67 and 76.28% on days 3, 7 and 10, respectively.

Any other information on results incl. tables

Table 1 Average % Theoretical CO₂ Production (>60% indicates passing conditions

	Agrol	Dextrose
Day 0	0	0
Day 3	66.72 + 0.296	57.17 + 6.391
Day 7	82.10 + 1.142	82.67 + 6.823
Day 10	89.17 + 6.071	76.28 + 5.305
Day 14	81.66 + 2.016	89.98 + 9.554
Day 17	79.05 + 2.468	85.05 + 3.361
Day 20	92.61 + 4.184	86.66 + 5.260
Day 24	86.02 + 2.499	78.69 + 9.562
Day 28	91.96 + 1.663	97.68 + 5.601

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Test product Agrol® achieved 91.96% biodegradation. The product meets method requirements for Ready Biodegradability.

Executive summary:

The ASTME 1720 -01 biodegradability testing monitors the degree of activity of microorganisms exposed to a material that is being tested for a biodegradable status. In the test, if the microorganisms recognize the material as a food source, then an increase in biological activity is observed through data collection specifically designed to assess biological conversion of organic carbon to inorganic carbon. If the material is not a recognizable food source or is toxic or inhibitory, then there is no measurable increase in biological activity or, in some cases, there is a marked decrease in activity relative to a biodegradable control.

 

Positive Control: Dextrose @10mg/L

Carbon Analyzer: High Sensitivity Shimadzu TOCvch

TIC Analysis Employed: Basified Aqueous Phase Inorganic Carbon via TOCvch

 

Test bottles were set up in triplicate for statistical validation of results,and a total of 9 sampling events were executed. The inoculum was preconditioned in the test medium via method guidance to remove organic carbon and inorganic carbon prior to testing. The inoculum source is the Escatawpa, MS municipal waste water treatment facility.

Based on the testing conducted in accordance with the specified method above, test product Agrol^® achieved 91.96% biodegradation. The product meets method requirements for Ready Biodegradability.

 

The method classifies greater than 60% biodegradation within a 10 day window as Ready Biodegradability and greater than 60% biodegradation over 28 days without meeting a 10 day window as Ultimate Biodegradability.