Manganese dichloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Not a standard test method, not to GLP. No data on dose groups etc, not basic observations made. Appears to follow basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Studies on the evaluation of the toxicity of various salts of lead, manganese, platinum and palladium

Author:

Holbrook DJ, Washington ME, Leake HB and Brubaker PE

Year:

1975

Bibliographic source:

Environmental Health Perspectives, 10: 95-101

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Two experiments were conducted, a lethal dose experiment and a dietary study.
In the lethal dose experiments salts were administered orally (via stomach tube) or intraperitoneally. The rats were observed through a 14 day observation period. LD50 values were calculated by the method of Litchfield and Wilcoxon.
In the dietary experiments, 4 rats were maintained per cage, the metallic salt under study was dissolved in the drinking water. Animals consumed feed and drinking fluid ad libitum. Analyses for metals were performed on samples from 3 lots of feed. The feed contained 56 ± 5 mg Mn / kg feed. Measurements were made of the body weights of individual rats and feed and fluid consumption per cage of four rats at 7-day intervals during the course of each dietary experiment. At the termination of the dietary experiments, samples of liver were used for the isolation of microsomes. Aniline hydroxylase was measured and modified by the addition of HgCl2. Aminopyridine demethylase was measured. Analyses of rat tissues for Mn concentration was conducted.

GLP compliance:

not specified

Test type:

other: Non standard. LD50 calculated by method of Litchfield and Wilcoxon.

Test material

Test material information

Constituent 1

Reference substance name:

Manganese dichloride

EC Number:

231-869-6

EC Name:

Manganese dichloride

Cas Number:

7773-01-5

Molecular formula:

Cl2Mn

IUPAC Name:

manganese(2+) dichloride

Details on test material:

Not reported

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

Animals were received at 3 - 3.5 weeks of age and maintained for 1 -1.5 weeks prior to use. The mean body weights were between 100 -110 g at the start of the studies.

Administration / exposure

Route of administration:

other: oral gavage and intraperitoneal

Vehicle:

water

Doses:

No data

No. of animals per sex per dose:

No data

Control animals:

yes

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

7.5 other: mmol / kg

95% CL:

7 - 8.1

Remarks on result:

other: oral gavage

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

0.7 other: mmol / kg

95% CL:

0.61 - 0.8

Remarks on result:

other: intraperitoneal

Effect levels 3

Sex:

male

Dose descriptor:

LD50

Effect level:

138 mg/kg bw

Remarks on result:

other: calculated from stated value of 0.61 - 0.80 mmol/kg bw

Effect levels 4

Sex:

male

Dose descriptor:

LD50

Effect level:

ca. 944 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Oral route mg/kg bw equivalent

Mortality:

No data

Clinical signs:

other: No data

Gross pathology:

Dietary study:
In rats treated for 90 -91 days, the control rats ingested approximately 0.15 g of manganese (from the solid feed), the tissue concentration of Mn was 1.4 and 1.0 µg Mn/g wet tissue in the liver and kidney respectively. In Mn-treated rats, which received 8.3mM MnCl2 as the drinking fluid and ingested approximately 2.3 g of Mn per rat during the 90 - 91 day interval, the concentration of Mn was increased to 2.8 and 1.6 µg Mn/g of wet tissue on the liver and kidney respectively. The Mn concentration in the spleen, heart, testes and blood was not increased in the tissues of Mn-treated rats.

Lethal dose experiment:
Rats were treated orally with a dose of MnCl2 equivalent to 100% of the oral LD50 value and the tissues were analysed in surviving rats at the end of the 14-day observation period. The oral administration of a single, large but nonlethal dose of MnCl2 to rats did not result in the retention after 14 days of excess concentrations of Mn in any of the tissues analysed. Levels were approximately equal to those found in the control animals.

Applicant's summary and conclusion

Conclusions:

Administration via the i.p. and oral route suggested that MnCl2 was harmful to rats. (i.p results indicated an approximate 10-fold higher toxicity than for the oral route).