Heptanoyl chloride

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2001

Report date:

2001

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes (incl. QA statement)

Remarks:

testing lab.

Test material

Test material information

Constituent 1

Reference substance name:

Heptanoyl chloride

EC Number:

219-775-3

EC Name:

Heptanoyl chloride

Cas Number:

2528-61-2

Molecular formula:

C7H13ClO

IUPAC Name:

heptanoyl chloride

Details on test material:

Name of the test substance used in the study report: n-Heptansaeurechlorid
Purity: 98.6%

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

The age of the animals at the beginning of the study was 8 - 9 weeks.
The animals were identified individually by color marking on the tail.
The animals were offered KLIBA rat/mouse/hamster laboratory diet 24-343-4 10 mm pellets, Klingentalmühle AG, Kaiseraugst, Switzerland, and drinking water ad libitum during the post-exposure observation period.
The animals were kept in fully air-conditioned rooms in which a temperature in the range of 20-24°C and relative humidity in the range of 30-70% were regulated by means of a central air-conditioning system.
The animals were housed singly in cages type DK III of Becker, without bedding, with a light/dark rhythm of 12 hours.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Duration of exposure:

4 h

Concentrations:

0.11, 0.34 and 0.51 mg/l

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

Duration of observation period following administration: 14 days
The body weight of the animals was determined just prior to exposure, after 7 days and at the end of the observation period. A check for overt clinical signs of toxicity or mortality as well as a check for feed and drinking water was made twice a day on workdays and once daily on weekends and public holidays. Detailed clinical observations were recorded for each animal separately several times during exposure and at least once on each workday in the observation period.
At the end of the 14-day observation period the surviving animals were sacrificed with CO2 and were subjected to gross-pathological examination like all other animals which had died before.

Statistics:

The statistical evaluation of the dose-response relationship was carried out using a computer program: Depending on the data of the dose-response
relationship obtained by way of experiment, this program is used to estimate the LC50 or to perform a Probit analysis. Estimation of the LC50 will produce types LC50 greater, LC50 about, or LC50 smaller. If the results are Type LC50 greater or LC50 smaller, an additional binomial test is carried out in order to verify these statements statistically, if necessary.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LC50

Effect level:

0.39 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 2

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 0.3 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Effect levels 3

Sex:

female

Dose descriptor:

LC50

Effect level:

ca. 0.51 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

At the end of the study
0.11 mg/l: no mortalities
0.34 mg/l: 4 males and no female
0.51 mg/l: 5 males and 2 females

Clinical signs:

other: Irregular, accelerated and intermittent respiration, respiratory sounds (only males), nose - discharge - bloody (only females), apathy, squatting posture, lateral position (only males), piloerection, smeared fur

Body weight:

Male animals:
0.11 mg/l - start of exposure 238 g, after 14 days 318 g
0.34 mg/l - start of exposure 255 g, after 14 days 302 g
0.51 mg/l - start of exposure 259 g, after 7 days all animals died

Female animals:
0.11 mg/l - start of exposure 196 g, after 14 days 221 g
0.34 mg/l - start of exposure 182 g, after 14 days 197 g
0.51 mg/l - start of exposure 190 g, after 14 days 207 g

Gross pathology:

Animals that died:
0.34 mg/l - lung: diffuse dark-red discolouration of all lobes.
0.51 mg/l - lung: diffuse dark-red discolouration of all lobes, retracted surface - severe

Surviving animals:
No abnormalities detected.