Heptanoyl chloride

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1998

Report date:

1998

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes (incl. QA statement)

Remarks:

testing lab.

Test type:

acute toxic class method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Heptanoyl chloride

EC Number:

219-775-3

EC Name:

Heptanoyl chloride

Cas Number:

2528-61-2

Molecular formula:

C7H13ClO

IUPAC Name:

heptanoyl chloride

Details on test material:

Name of the test substance used in the study report: n-Heptansaeurechlorid
Degree of purity/content: 98.6 g/100 g (GC)

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

Young adult rats were used for this experiment with a comparable body weight at the start of the study of 150 - 300 g. The animals were identifed using cage cards and group identification by tail marking. They were housed in fully air-conditioned rooms with central air-conditioning in a range of 20-24°C for room temperature and a relative humidity of 30-70%. The day/night rhythm was 12 hours dark and 12 hours light. Stainless steel wire mesh cages (type DK-III) were used (singly housing). A standardized animal diet and drinking water were available ad libitum. The acclimatization period was at least 1 week. The animals were given no feed at least 16 hours before administration (water was available).

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

The test substance is sensitive to hydrolysis; good solubility in olive oil DAB 10.

Doses:

200 and 2000 mg/kg

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

Duration of observation period following administration: 15 days (200 mg/kg) resp. 14 days (2000 mg/kg)
Individual body weights were reported shortly before administration, thereafter weekly and at the end of the study.
Recording of signs and symptoms were done several times on the day of administration, at least once each workday for the individual animals.
A check for any dead or moribund animal was made twice each working day and once on weekends or on public holidays.
Necropsy on the last day of the observation period with withdrawal of food at least 16 hours before killig with CO2; then necropsy of all animals that died as soon as possible.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

none

Clinical signs:

other: Impaired and poor general state, dyspnoea, apathy, abdominal position (only females), staggering, ataxia, atonia (only females), paresis (only females), tremor (only males), cyanosis (only males), piloerection, salivation (only males), compulsary gnawing

Gross pathology:

No pathological findings noted.