Registration Dossier
Registration Dossier
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EC number: 608-035-9 | CAS number: 27014-42-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP without deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Poly(oxy-1,2-ethanediyl), a,a',a'',a'''-[1,2-ethanediylbis(nitrilodi-2,1-ethanediyl)]tetrakis[w-hydroxy-
- EC Number:
- 608-035-9
- Cas Number:
- 27014-42-2
- IUPAC Name:
- Poly(oxy-1,2-ethanediyl), a,a',a'',a'''-[1,2-ethanediylbis(nitrilodi-2,1-ethanediyl)]tetrakis[w-hydroxy-
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- Origin: wastewater treatment plant Hildesheim - clarifier
Bacterial density of the inoculum: 49x10^3 colony-forming units / mL
Preparation: The activated sludge was filtered through a fluted filter. The first 200 mL were discarded. The filtrate was kept under aerobic conditions to beginning of the test . - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 60 mg/L
- Based on:
- test mat.
- Details on study design:
- The necessary amounts of nutrient medium, test- and reference compounds were dissolved in 2 L flasks and filled with aqua demin.
1000 [mL] were filled into a 2 L Erlenmeyer flasks and inoculated with 0.5 [mL] inoculum. At the beginning of the test the pH was checked and adjusted to pH 7.4 ± 0.1
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
% Degradation
- Parameter:
- % degradation (DOC removal)
- Value:
- 4
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- The reference substance was fully degrated at day 14. (93% at day 7)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- other: not ready biodegradable
- Conclusions:
- The substance is according to OECD301E test not ready biodegradable.
- Executive summary:
The substance is according to OECD301E test not ready biodegradable.
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