4-aminoazobenzene

Specific investigations: other studies

Administrative data

Endpoint:

methaemoglobinaemia

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable publication which meets basic scientific principles

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Methemoglobin induced by carcinogenic aminoazo dyes in rats

Author:

Lin JK, Hsu SM, Wu YH

Year:

1972

Bibliographic source:

Biochem Pharmacol 21: pp. 2147-50

Reference 2

Reference Type:

secondary source

Title:

4-Aminoazobenzene. BUA-Stoffbericht 217

Author:

Beratergremium für Altstoffe (BUA)

Year:

1998

Bibliographic source:

BUA der Gesellschaft dt. Chemiker, S. Hirzel Wissenschaftliche Verlagsgesellschaft, Stuttgart

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Application of 4-Aminoazobenzene intraperitoneally. Analysis of hematological changes.

GLP compliance:

no

Type of method:

in vivo

Test material

Test material information

Constituent 1

Reference substance name:

4-aminoazobenzene

EC Number:

200-453-6

EC Name:

4-aminoazobenzene

Cas Number:

60-09-3

Molecular formula:

C12H11N3

IUPAC Name:

4-[(E)-2-phenyldiazen-1-yl]aniline

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 200-260g or 250-300g, respectively

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

peanut oil

Analytical verification of doses or concentrations:

not specified

Duration of treatment / exposure:

after i.p. application analysis after 1h, 4h, 7h, and 10h

Frequency of treatment:

single dose

Post exposure period:

not applicable

Doses / concentrations

Remarks:

Doses / Concentrations:
0.323, 0.161, 0.08, 0.04, 0.02 mmol/kg
Basis:
other: nominal dose

No. of animals per sex per dose:

1h: n=10; 4h: n=5; 7h: n=5; 10h: n=5
0.323mmol/kg: n=10 (after 1h), n=5 (after 4h)
0.161mmol/kg: n=4 (after 1h), n=4 (after 4h)
0.080mmol/kg: n=4 (after 1h), n=4 (after 4h)
0.040mmol/kg: n=3 (after 1h), n=3 (after 4h)
0.020mol/kg: n=3 (after 1h), n=3 (after 4h)

Control animals:

not specified

Details on study design:

Formation of methemoglobin. The concentration of methemoglobin (as percentage of total hemoglobin) in the blood of rats injected i.p. with Aminoazobenzene in 0.5ml peanut oil were determined. After the tested compounds were injected, the blood (0.5 - 0.7ml) of rats was collected at a given time interval by heart puncture under ether anesthesia.

Examinations

Examinations:

The blood was transferred to an oxalated tube (potassium oxalate, 1.5mg/ml of blood) and mixed thoroughly by gentle swirling. The concentration of methemoglobin in the collected sample was determined within 20 min, according to a spectrophotometric method, and calculated.

Positive control:

no data

Results and discussion

Any other information on results incl. tables

1 hour after injection 66.6% of total hemoglobin was detected as methemoglobin. Level decreased time-dependent. 10 hours after injection Met-HB level was 7.2%.

Aminoazobenzene	Methemoglobin (% of total Hb)
mmol/kg	1hour	4 hours
0.323	66.6 ± 5.2	44.5 ± 6.2
0.161	53.1 ± 3.1	39.6 ± 2.6
0.080	48.9 ± 4.8	31.4 ± 3.7
0.040	24.6 ± 3.0	6.2 ± 0.2
0.020	18.5 ± 1.1	2.2 ± 0.2