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EC number: 260-300-4 | CAS number: 56634-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 - 17 Jul 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 405 (Acute Eye Irritation / Corrosion)
- Version / remarks:
- adopted 2021
- Deviations:
- yes
- Remarks:
- Age and weight of animals not reported, environmental and general conditions of animals not reported, no data on untreated eye, no anesthetics and systemic analgesics were applied
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-4 (Acute Eye Irritation)
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,6-dibromo-4-cyanophenyl heptanoate
- EC Number:
- 260-300-4
- EC Name:
- 2,6-dibromo-4-cyanophenyl heptanoate
- Cas Number:
- 56634-95-8
- Molecular formula:
- C14H15Br2NO2
- IUPAC Name:
- 2,6-dibromo-4-cyanophenyl heptanoate
Constituent 1
Test animals / tissue source
- Species:
- rabbit
- Strain:
- New Zealand White
- Details on test animals or tissues and environmental conditions:
- TEST ANIMALS
- Source: Ray Nichols Rabbitry, Lumberton, Texas, USA
- Age at study initiation: young adults
- Weight at study initiation: not reported
- Housing: individually in suspended, wire bottom, stainless steel cages
- Diet: Purina Rabbit Chow, ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least one week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported
IN-LIFE DATES: From: 10 Jul 1989 To: 17 Jul 1989
Test system
- Vehicle:
- unchanged (no vehicle)
- Controls:
- yes, concurrent no treatment
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied: 0.1 mL
- Concentration: 100% actual test material as received
- The test item has been liquified by heating to 45 °C prior to treatment. - Duration of treatment / exposure:
- 30 seconds (wash group)
continuous treatment (non-wash group) - Observation period (in vivo):
- 7 days
- Number of animals or in vitro replicates:
- non-wash group: 3 per sex
wash group: 3 males - Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Washing: either no washing was performed (non-wash group) or the treated eye was rinsed with deionized water for one minute (wash group)
- Time after start of exposure: 30 seconds (wash group only)
SCORING SYSTEM: Draize scoring system
TOOL USED TO ASSESS SCORE: 0.2% fluorescein sodium ophthalmic solution
Results and discussion
In vivo
Resultsopen allclose all
- Irritation parameter:
- chemosis score
- Basis:
- mean
- Remarks:
- of two animals scored (wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Remarks on result:
- other: one male was found dead on Day 1
- Irritation parameter:
- chemosis score
- Basis:
- animal #1
- Remarks:
- male (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #2
- Remarks:
- male (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 4
- Reversibility:
- fully reversible within: 72 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #3
- Remarks:
- male (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritation parameter:
- chemosis score
- Basis:
- animal #4
- Remarks:
- female (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #5
- Remarks:
- female (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- chemosis score
- Basis:
- animal #6
- Remarks:
- female (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.3
- Max. score:
- 4
- Reversibility:
- fully reversible within: 48 h
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Remarks:
- of two animals scored (wash group)
- Time point:
- 24/48/72 h
- Score:
- 0.7
- Max. score:
- 3
- Reversibility:
- fully reversible within: 72 h
- Remarks on result:
- other: one male was found dead on Day 1
- Irritation parameter:
- conjunctivae score
- Basis:
- mean
- Remarks:
- of all six animals (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 1
- Max. score:
- 3
- Reversibility:
- fully reversible within: 4 to 7 days
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- of two animals scored (wash group)
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Remarks on result:
- other: one male was found dead on Day 1
- Irritation parameter:
- iris score
- Basis:
- mean
- Remarks:
- of all six animals (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 2
- Reversibility:
- other: not applicable
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- of two animals scored (wash group)
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Remarks on result:
- other: one male was found dead on Day 1
- Irritation parameter:
- cornea opacity score
- Basis:
- mean
- Remarks:
- of all six animals (non-wash group)
- Time point:
- 24/48/72 h
- Score:
- 0
- Max. score:
- 4
- Reversibility:
- other: not applicable
- Irritant / corrosive response data:
- No effects on iris and cornea opacity were noted in any animal (wash and non-wash group). The test item induced conjunctivae redness in all animals of the non-wash group at the 1 h reading time point (6/6 animals scored grade 1). Full reversibility was observed after 4 (3/6 animals) to 7 days (3/6 animals). Chemosis was also observed at the 1 h reading time point for the non-wash group; 6/6 animals were scored grade 1 for this endpoint. In one animal the effect was already reversed after 24 h, in 3/6 animals after 48 h and in the remaining 2 animals after 72 h. In the wash group, conjunctivae redness was observed in all animals at the 1 h reading time point (grade 1) and full reversibility was observed after 72 h. In the same group, chemosis of grade 1 was noted in all three animals 1 h after exposure lasting until the 48 h reading time point.
- Other effects:
- One male of the wash group was found dead on Day 1 of the study (24 h after exposure). Positive fluorescein staining did not occur in any of the non-washed or washed eyes.
Applicant's summary and conclusion
- Interpretation of results:
- other: CLP/EU GHS criteria not met, no classification required according to Regulation (EC) No 1272/2008
- Conclusions:
- The study is in accordance with EPA guideline no. 81-4 and was conducted under GLP conditions. Although the study possesses deviations from the current OECD guideline no. 405 (eye irritation), such as missing information on age and weight of animals, environmental and general conditions of anima, no anesthetics and systemic analgesics were applied; in some animals washing occured 30 sec after substance instillation, it is considered valid and reliable. Under the conditions chosen the test substance did not induce eye irritation to rabbits. According to criteria of the CLP Regulation (EU) No. 1272/2008, no classification of the test item for eye irritation is required.
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