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EC number: 260-300-4 | CAS number: 56634-95-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 31 Aug - 21 Sep 1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 989
- Report date:
- 1989
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Version / remarks:
- adopted 2009
- Deviations:
- yes
- Remarks:
- Limited information on environmental conditions, exposure and test atmosphere generation.
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 81-3 (Acute inhalation toxicity)
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- 2,6-dibromo-4-cyanophenyl heptanoate
- EC Number:
- 260-300-4
- EC Name:
- 2,6-dibromo-4-cyanophenyl heptanoate
- Cas Number:
- 56634-95-8
- Molecular formula:
- C14H15Br2NO2
- IUPAC Name:
- 2,6-dibromo-4-cyanophenyl heptanoate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Harlan Sprague Dawley, Inc., Houston, Texas
- Females nulliparous and non-pregnant: yes
- Age at study initiation: young adult
- Weight at study initiation: 299 - 370 g (males), 176 - 240 g (females)
- Housing: in groups of 1-3, separated by sex, in suspended, wire bottom, stainless steel cages
- Diet: Purina Formulab Chow #5008, ad libitum
- Water: tap water, ad libitum
- Acclimation period: at least one week
ENVIRONMENTAL CONDITIONS
- Temperature (°C): not reported
- Humidity (%): not reported
- Air changes (per hr): not reported
- Photoperiod (hrs dark / hrs light): not reported
IN-LIFE DATES: From: 31 Aug To: 21 Sep 1989
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- not specified
- Vehicle:
- air
- Mass median aerodynamic diameter (MMAD):
- > 1.651 - < 1.978 µm
- Geometric standard deviation (GSD):
- > 1.866 - < 2.279
- Remark on MMAD/GSD:
- Details can be found in Attachment 1.
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: 200 L New York University design, stainless steel, dynamic flow inhalation chamber
- Exposure chamber volume: 200 L
- Method of holding animals in test chamber: not specified
- Source and rate of air (airflow): 73.3 - 77.6 L/min
- System of generating particulates/aerosols: aerosol was generated by pumping the test material through a pressure operated spray nozzle. This was then diluted with dried and filtered air and drawn into the exposure chamber.
- Method of particle size determination: Particle size determinations were made using an Andersen cascade impactor.
- Temperature, humidity, pressure in air chamber: Temperature and humidity were recorded at 30 minute intervals during the exposure period from a Taylor wet bulb/dry bulb hygrometer located in the exposure chamber, temperature ranged from 22.8 - 25.5 °C, relative humidity from 59 - 75%, air pressure was not specified.
TEST ATMOSPHERE
- Brief description of analytical method and equipment used: Analysis of the concentration of the test atmosphere was performed according to a modification of the method provided by the sponsor using a Tracer Model 560 gas chromatograph. The nominal concentration was determined by dividing the loss in weight of the test material after each exposure by the total volume of air which passed through the chamber.
- Time needed for equilibrium of exposure concentration before animal exposure: not reported
- Particle size distribution: Please refer to Attachment 1 - Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- Nominal: 1.94 , 8.54 and 21.1 mg/L
Measured: 0.523, 1.45 and 2.69 mg/L - No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: on the day of exposure and at least once daily thereafter. Due to chamber design, only 4 animals (2 males and 2 females, for example) could be observed during the exposure period. Observations after four hours included all animals.
- Frequency of weighing: just prior to the inhalation exposure and on Days 7 and 14 or at the time of discovery after death
- Necropsy of survivors performed: yes
- Clinical signs including body weight: yes - Statistics:
- None
Results and discussion
Effect levelsopen allclose all
- Key result
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 1.975 mg/L air
- Based on:
- test mat.
- 95% CL:
- 1.433 - 2.722
- Exp. duration:
- 4 h
- Remarks on result:
- other: Mortality occurred at the mid and high concentration levels of 1.45 and 2.69 mg/L air, respectively.
- Key result
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 1.479 mg/L air
- Based on:
- test mat.
- 95% CL:
- 0.748 - 2.924
- Exp. duration:
- 4 h
- Remarks on result:
- other: Mortality occurred at the mid and high concentration levels of 1.45 and 2.69 mg/L air, respectively.
- Key result
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 1.717 mg/L air
- Based on:
- test mat.
- 95% CL:
- 1.267 - 2.327
- Exp. duration:
- 4 h
- Remarks on result:
- other: both sexes combined
- Mortality:
- - 0.523 mg/L: no mortality observed
- 1.45 mg/L: 1/5 males died, 3/5 females died
- 2.91 mg/L: 4/5 males died, 4/5 females died - Clinical signs:
- other: clinical signs of toxicity in males and females almost included nasal discharge, salivation, lacrimation, piloerection, ptosis, and decreased activity; for details, please refer to "any other Information on results incl. tables", and to Attachment 2.
- Body weight:
- - 0.523 mg/L: all animals achieved body weight gains as expected during the 14 day observation period.
- 1.45 mg/L: surviving animals achieved body weight gains as expected over the 14 day observation period despite of a lost in weight noticed during the first week.
- 2.69 mg/L: the 2 surviving animals achieved body weight gains as expected over the 14 day observation period despite of a lost in weight noticed during the first week..
Summarized results can be found in Attachment 3. - Gross pathology:
- - 0.523 mg/L: No pathological findings were reported.
- 1.45 mg/L: lungs mottled red (1 male and 3 females); orange and red mucoid material in small intestine (1 male and 2 females)
- 2.69 mg/L: lungs red or dark red (3 males, 3 females); orange mucoid material in small intestine (2 males, 2 females), tan slurry and dark brown slurry in stomach (2 males), gastrointestinal tract empty (1 female), dark red mucoid material in small intestine (1 female)
Summarized results can be found in Attachment 3. - Other findings:
- None
Any other information on results incl. tables
Details on clinical signs of toxicity:
- 0.523 mg/L: clinical signs of toxicity for both, males and females,
consisted of ptosis, decrease in activity and piloerection; the signs
were all reversible.
- 1.45 mg/L: nasal discharge, salivation, lacrimation, piloerection,
decrease in activity and ptosis were the more common signs of toxicity
seen in males and females at this concentration level.
- 2.69 mg/L: also at this concentration level, the clinical signs of
toxicity almost consisted of nasal discharge, salivation, lacrimation,
piloerection, decrease in activity and ptosis for both, males and
females.
Please also refer to Attachment 2.
Applicant's summary and conclusion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- The study was conducted under GLP conditions, according to U.S. EPA guidelines No. 81-3, which in principle is similar to the OECD test guideline 403. The animals were exposed for 4 hours to the test item as aerosol. The resulting combined LC50 for male and female rats was set at 1.72 mg/L air. Accordingly, the substance is to be classified in Cat 4 according to the criteria of the the CLP Regulation (EU) No. 1272/2008.
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