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EC number: 241-646-5 | CAS number: 17671-27-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 03 Dec - 07 Dec 2012
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- GLP - Guideline study, tested with the source substance dodecyl oleate (CAS 36078-10-1). According to the ECHA guidance document “Practical guide 6: How to report read-across and categories (March 2010)”, the reliability was changed from RL1 to RL2 to reflect the fact that this study was conducted on a read-across substance.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- LUBW Landesanstalt für Umwelt, Messungen und Naturschutz, Baden-Württemberg, Germany
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from the control and the exposure vessel (100 mg/L).
- Sampling method: Sampling at start (0 h) and end (96 h), 4.8 mL were taken from the test and the control aquarium for chemical analysis.
- Sample storage conditions before analysis: Sample storage in 4 mL brown glass vials (filled up to the brim to avoid oxidative processes), maximum storage period of 8 d at 5 °C ± 3 °C before analysis. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Due to sparingly soluble properties of the test item, the study was performed with the water accommodated fraction (WAF) prepared with synthetic fish water according to OECD 203. The test solutions were withdrawn from the middle of the suspension og the beakers after 1 h settling using a glass tube and transferred into the test vessels (aquarium).
Two beakers were prepared like this and stirred for a period of 48 h at 12-22 °C in diffuse light. For stirring an overhead high-grade stainless steel radial impeller was used (about 500 rpm). The impeller was placed about 5 cm above the bottom of the beakers. The depth of the vortex was about 10% of the total height of the liquid. The test item is very mobile and was therefore added by pipetting. According to the density indicated by the sponsor of 0.8529 g/cm3 the amount to be added was converted into volume.
The filtration of the WAF to obtain the water soluble fraction (WSF) was not taken into consideration to avoid unwanted impacts of the filtration process and to have a worst case scenario.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Slightly whitish oily spots were still floating on the surface of the solutions. The test solutions were withdrawn from the middle of the suspension on the beakers using a glass tube and transferred into the test vessels (aquarium). - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Aquarium Dietzenbach Fischzucht, Dietzenbach, Germany, delivery date: 20 Nov 2012
- Length at study initiation (length definition, mean, range and SD): 2 ± 1 cm
- Feeding during test: none
ACCLIMATION
- Acclimation period: 8 days before the test started.
- Acclimation conditions (same as test or not): same as test
- Feeding frequency: 2 x every working day.
- Health during acclimation (any mortality observed): No mortality, the fish behaved normally. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Test temperature:
- 22.3 - 23.9 °C
- pH:
- 8.0 - 8.1
- Dissolved oxygen:
- Control: 7.9 - 8.6 mg O2/L
100 mg/L: 7.9 - 8.7 mg O2/L - Nominal and measured concentrations:
- Nominal: control, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Size: 8 L aquarium
- Aeration: yes
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Culture medium different from test medium: no
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 14 h light / 10 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality every 24 h (and 6 h after study initiation); behavioural abnormalities - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no
- Mortality of control: no - Sublethal observations / clinical signs:
No fish died or showed any abnormal behaviour during the 96 h exposure period.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07 Mar - 11 Mar 1994
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study with acceptable restrictions (Insoluble test substance was visible during the test).
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.1 (Acute Toxicity for Fish)
- Deviations:
- yes
- Remarks:
- Fish were fed during the main-test
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations: Samples were taken from 1000 and 10000 mg/L test system after 0 h and 24 h.
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was directly weighed into the test vessels and treated with ultraturrax for 10 sec.
- Eluate: no
- Differential loading: yes
- Controls: yes, water control - Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Details on test organisms:
- TEST ORGANISM
- Common name: zebra fish
- Source: Obtained from Fa. Westaquarium, Germany (delivered on 15 Dec 1993)
- Feeding during test
- Food type: Altromin N 1324 spezial 161293 1500 (preliminary study); Altromin N 1324 spezial 140694 0825 (main study) - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Test temperature:
- 21.5 - 23.1 °C
- pH:
- 8.1 - 8.6
- Dissolved oxygen:
- 67 - 89%
- Nominal and measured concentrations:
- Nominal: control - 1000 - 3000 - 10000 mg/L
Measured (DOC analysis):
1000 mg/L (=39.2 mg DOC/L): 2.1 mg DOC/L (0 h); 2.0 mg DOC/L (24 h); 1.0 mg DOC/L (0 h); 1.5 mg DOC/L (24 h)
10000 mg/L (=392 mg DOC/L): 2.8 mg DOC/L (0 h); 2.0 mg DOC/L (24 h); 1.2 mg DOC/L (0 h); 1.5 mg DOC/L (24 h) - Details on test conditions:
- TEST SYSTEM
- Fill volume: 5 L
- Renewal rate of test solution (frequency): every 24 h
- No. of organisms per vessel: 10
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: drinking water
OTHER TEST CONDITIONS
- Adjustment of pH: no
EFFECT PARAMETERS MEASURED: mortality was checked after 0, 4-6, 24, 48, 72 and 96 h of exposure - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no behavioural effects were observed
- Mortality of control: 0%
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: The test substance was not completely soluble in water and floated on top of the surface of the test solutions. - Sublethal observations / clinical signs:
Table 1: Percental mortality of fish during the study
Concentration [mg/L]
% mortality after x hours
0 h
4-6 h
24 h
48 h
72 h
96 h
0
0
0
0
0
0
0
1000
0
0
0
0
0
0
3000
0
0
0
0
0
0
10000
0
0
0
0
0
0
- Endpoint:
- short-term toxicity to fish
- Type of information:
- read-across from supporting substance (structural analogue or surrogate)
- Adequacy of study:
- key study
- Justification for type of information:
- Please refer to analogue justification provided in IUCLID section 13.
- Reason / purpose for cross-reference:
- read-across source
- Reason / purpose for cross-reference:
- read-across source
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- other: Water Accommodated Fraction (WAF)
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Source: key, RA-A, CAS 36078-10-1, Dako, 2013, D. rerio
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other:
- Remarks:
- Source: key, RA-A, CAS 95912-87-1, BASF, 1994, B. rerio
Referenceopen allclose all
Description of key information
No effects up to the limit of water solubility (EU Method C.1; OECD 203).
Key value for chemical safety assessment
Additional information
Since no studies investigating the short-term toxicity of docosyl docosanoate (CAS 17671-27-1) to fish are available for this endpoint, in accordance to Regulation (EC) No. 1907/2006 Annex XI, 1.5 a read across to the two structurally related read-across substances dodecyl oleate (CAS 36078-10-1) and fatty acids, C16-18, C12-18-alkyl esters (CAS 95912-87-1) was conducted. The read-across substance dodecyl oleate is characterized by a smaller fatty acid chain (C18 unsatd. compared to C22 unsatd. (target)) and a smaller fatty alcohol chain length (C12 compared to C18 unsatd. (target)). The second read-across substance fatty acids, C16-18, C12-18-alkyl esters is a UVCB substance showing a similar structure to the target substance. The smaller alcohol and fatty acid chain length increases the water solubility and therefore the bioavailability in the water phase. Water solubility is negatively correlated with the C-chain length of the fatty alcohol and fatty acid (Lide, 2005). Therefore, it is possible to extrapolate from a source substance with higher water solubility since it has an increased bioavailability in the water phase (ECHA, 2017). This read-across is justified in detail in the overall summary (IUCLID section 6.1) and within the analogue justification in IUCLID Section 13. In this case of read-across, the best suited (highest degree of structural similarity, nearest physico-chemical properties) read-across substance was used for the assessment. Nevertheless, as it can be seen in the data matrix of the analogue justification in IUCLID section 13 and the overall summary, all reliable data support the hazard assessment by showing a consistent pattern of results.
The first study conducted with the read-across substance fatty acids, C16-18, C12-18-alkyl esters (CAS 95912-87-1) was performed according to the EU Method C.1 (GLP) with the zebrafish Brachydanio rerio (Stelter, 1994). A static exposure regime was chosen. Three concentrations of nominal 1000, 3000 and 10000 mg/L were directly applied to the test vessels without a filtration step. Undissolved test material floated on top of the water surface but no effects were observed by the dissolved and even by the undissolved test substance present in the water. Even though the undissolved particles were not removed this approach can be considered as a worst case since possible effects of dissolved and undissolved fractions were evaluated. Thus, a LC50 (96 h) of > 10000 mg/L (nominal) was derived.
The second study with the read-across substance dodecyl oleate (CAS 36078-10-1) was performed according to OECD 203 under GLP conditions with the zebrafish Danio rerio (Hafner, 2013). A limit test with a Water Accommodated Fraction (WAF) of 100 mg/L (nominal) did not result in any mortality after 96 h. Thus, a LL50 (96 h) of > 100 mg/L was derived based on the nominal loading rates. The chemical analysis resulted in concentrations below the analytical detection limit of 0.005 mg/L reflecting the very low water solubility of the test item.
Based on the available results from two structurally related read-across substances (in accordance to Regulation (EC) No 1907/2006 Annex XI, 1.5) which are characterized by a similar ecotoxicological profile and comparable structure, it can be concluded that docosyl docosanoate (CAS 17671-27-1) will not exhibit short-term effects to fish up to the limit of water solubility.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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