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Environmental fate & pathways

Biodegradation in water: screening tests

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biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
June 2013 - August 2013
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
according to guideline
OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
Version / remarks:
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Chemical name: (Z)-1-chloro-2,3,3,3-tetrafluoropropene
Other name: HCF0-1224yd(Z)
Molecular formula: C3HClF4
Molecular weight: 148.49
Purity of test item: >96.42% (GC area%)
Impurity: (E)-1-Chloro-2,3,3,3-tetrafluoropropene 2.73% (GC area%)
1,1,1,2,-Tetrafluoropropane 0.03% (GC area%)
2-Chloro-1,3,3,3-tetrafluoropropene 0.02% (GC area%)
Unknown (0.80%) (GC area%)
Lot number: 120517D-Fr9
The test item was treated as 100% in purity.

Test sample was stored in a dark storage place at room temperature.
Oxygen conditions:
Inoculum or test system:
sewage, domestic (adaptation not specified)
Details on inoculum:
Secondary effluent of city sewage treatment plant, Kurume Central sewage treatment center (Kurume-shi, Fukuoka, Japan). Sampling date: June 12, 2013
Duration of test (contact time):
28 d
Initial conc.:
7.78 mg/L
Parameter followed for biodegradation estimation:
DOC removal
Details on study design:
Concentration of test item: 7.78 mg/L
Concentration of inoculum: One drop/L
Volume of test solution: 100mL
Incubation temperature: 20+-C
Incubation method: Stationary incubation in sealed condition
Incubation duration: 28d (under dark condition)

Measurement of dissolved oxygen by diaphragm electrode method
Determination of test item by gas chromatography
Reference substance:
benzoic acid, sodium salt
Key result
% degradation (DOC removal)
Sampling time:
28 d
Details on results:
An almost theoretical amount of the test item remained in the abiotic control (water + test item) and the test suspension (inoculum + test item). The average percentage biodegradation by BOD was 0% after 28 days. No peak except the test item peak was detected on the GC chromatograms. This result showed that there was no degradation product. From the above it was concluded that the test item was not biodegraded under the test conditions of this study.
0 mg O2/g test mat.
Results with reference substance:
The average percentage biodegradation of the inoculum and sodium benzoate was 74% after 28 days.


* The average percentage biodegradation was regarded as 0% because the calculated average value in parentheses was negative.

  Test suspension (inoculum + test item)
 Percentage biodegradation by BOD  -3  -4  average  0 (-3)*%
Percentage biodegradation of test item (GC)   0  0  average  0%
Validity criteria fulfilled:
Interpretation of results:
under test conditions no biodegradation observed
The test item was not biodegraded under the test conditions of this study.
Executive summary:

HCFO-1224yd(Z) was not biodegraded under the test conditions of this study.

Description of key information

No biodegradation was observed over the 28d period for HCFO-1224yd(Z)

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information