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EC number: 204-524-2 | CAS number: 122-14-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 13 July 1988 to 03 August 1988
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 988
- Report date:
- 1988
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- EPA OPP 72-4 (Fish Early Life-Stage and Aquatic Invertebrate Life-Cycle Studies)
- Deviations:
- no
- Principles of method if other than guideline:
- A dynamic 21-day life cycle toxicity study of Fenitrothion Technical to Daphnia magna was conducted to determine the Maximum Acceptable Toxicant Concentration (MATC) limits. Each concentration consisted of four replicates containing 10 daphnids for a total of 40 daphnids. Exposure concentrations of Fenitrothion Technical were measured analytically on days -n, 0, 4, 7, 10, 14, 17 and 21. All results were based on the mean measured concentrations of Fenitrothion Technical.
- GLP compliance:
- yes
- Remarks:
- (Self certification by the laboratory)
Test material
- Reference substance name:
- Fenitrothion
- EC Number:
- 204-524-2
- EC Name:
- Fenitrothion
- Cas Number:
- 122-14-5
- Molecular formula:
- C9H12NO5PS
- IUPAC Name:
- O,O-dimethyl O-3-methyl-4-nitrophenyl phosphorothioate
- Test material form:
- liquid
Constituent 1
- Specific details on test material used for the study:
- Fenitrothion (Batch No.: 60553, Purity: 94.5%, Specification No. 01 - Document J)
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Exposure concentrations of fenitrothion were determined on days-N (analytical sampling prior to study initiation), 0, 4, 7, 10, 14, 17 and 21. The mean measured concentrations were 0.029, 0.042, 0.087, 0.23 and 0.44 µg/l. These were 102 ± 16% of the nominal concentrations.
Test solutions
- Vehicle:
- yes
- Remarks:
- acetone
- Details on test solutions:
- A half-liter proportional diluter system was used to maintain constant test concentrations. The nominal concentrations were 0.024, 0.048, 0.10, 0.20 and 0.40 µg/l. The mean measured concentrations, were determined by GLC: 0.029, 0.042, 1.87, 0.23 and 0.44 µg/l. The mean measured levels averaged 102 ±16% of expected nominal concentrations .
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- In-house culture. The primary culture was obtained from the Columbia National Fisheries Research Laboratory.
Study design
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Hardness:
- 160-180 mg/lCaCO3
- Test temperature:
- 19-20°C
- pH:
- 7.7-8.2
- Dissolved oxygen:
- 7.3-8.4 mg/L
- Nominal and measured concentrations:
- The nominal concentrations were 0.024, 0.048, 0.10, 0.20 and 0.40 µg/l.
The mean mesured concentrations were 0.029, 0.042, 0.087, 0.23 and 0.44 µg/l - Details on test conditions:
- Temperature, which was recorded continuously, ranged from 19-21°C throughout the daily observations of the study. The dissolved oxygen concentrations which ranged from 7.3 to 8.4 mg/l, represented which between 84 and 94% saturation at 20° and 19°C. These values were considered adequate for testing and no supplemental aeration was necessary. The pH values of the treated chambers were consistent with the control throughout the study, ranging from 7.7 to 8.2.
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.19 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality
- Duration:
- 21 d
- Dose descriptor:
- other: point estimate MATC
- Effect conc.:
- 0.14 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: survival, young/adult/repro duction day, adult mean length and length in days to first brood
- Key result
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.087 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: survival, young/adult/repro duction day, adult mean length and length in days to first brood
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.23 µg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- other: survival, young/adult/repro duction day, adult mean length and length in days to first brood
- Details on results:
- Statistical analysis of survival for Daphnia magna after a 21-day exposure to Fenitrothion Technical indicated that daphnid survival was significantly different (P<0.05) from the pooled controls in the mean measured concentrations of 0.23 and 0.44 µg/l. A day 21 EC50 was calculated to be 0.19 µg/l. The mean young/adult/reproduction day for 21 days were significantly affected in the mean measured exposure levels of 0.23 and 0.44 µg/l of Fenitrothion Technical .
The daphnid lengths in the Fenitrothion Technical mean measured concentration of 0.23 µg/l were significantly different from the pooled controls. Length measurements could not be made on the 0.44 µg/l test level daphnids since all had died by day 12 of the study.The time in days to first brood for the controls and all treatment levels was 9.0 days. Therefore, no significant difference occurred in time to first brood for any test level.
Based on the statistical analysis of survival, young/adult/repro duction day, adult mean length and length in days to first brood from this 21-day Daphnia magna dynamic life cycle study, the MATC limits were estimated to be 0.087 and 0.23 µg/l . The point estimate MATC value was 0.14 µg/l.
Any other information on results incl. tables
Adult daphnid length: The length of adultDaphnia magnain the control and solvent control were not significantly different, so they were pooled.
A one-way analysis of variance and Dunnett's multiple means test indicated that the daphnid length in the 0.23 µg/l dosage was significantly different from those of the pooled controls. Since all daphnids had died in the 0.44 µg/l test level by day 12, no length data was available for statistical analysis.
Young/adult reproduction days (Total number of young produced/Total number of reproduction days):
Young/adult reproduction days in the control and solvent control were not significantly different, so they were pooled.
The mean young/adult reproduction days were significantly reduced in the 0.23 and 0.44 µg/l dosages. All young produced during this study appeared normal in all test concentrations.
Time to first brood: The time in days to first brood for the controls and all treatment levels was 9 days.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- A day 21 EC50 was calculated to be 0.19 µg/l. The mean young/adult/reproduction day for 21 days were significantly affected in the mean measured exposure levels of 0.23 and 0.44 µg/l of Fenitrothion Technical.
The daphnid lengths in the Fenitrothion Technical mean measured concentration of 0.23 µg/l were significantly different from the pooled controls. Length measurements could not be made on the 0.44 µg/l test level daphnids since all had died by day 12 of the study.
The time in days to first brood for the controls and all treatment levels was 9.0 days. Therefore, no significant difference occurred in time to first brood for any test level.
Based on the statistical analysis of survival, young/adult/repro duction day, adult mean length and length in days to first brood from this 21-day Daphnia magna dynamic life cycle study, the MATC limits were estimated to be 0.087 (NOEC) and 0.23 µg/l (LOEC) . The point estimate MATC value was 0.14 µg/l. - Executive summary:
A dynamic 21-day life cycle toxicity study of Fenitrothion Technical to Daphnia magna was conducted to determine the Maximum Acceptable Toxicant Concentration (MATC) limits. Each concentration consisted of four replicates containing 10 daphnids for a total of 40 daphnids. Exposure concentrations of Fenitrothion Technical were measured analytically on days -n, 0, 4, 7, 10, 14, 17 and 21. All results were based on the mean measured concentrations of Fenitrothion Technical.
A day 21 EC50 was calculated to be 0.19 µg/l. The mean young/adult/reproduction day for 21 days were significantly affected in the mean measured exposure levels of 0.23 and 0.44 µg/l of Fenitrothion Technical . The daphnid lengths in the Fenitrothion Technical mean measured concentration of 0.23 µg/l were significantly different from the pooled controls. Length measurements could not be made on the 0.44 µg/l test level daphnids since all had died by day 12 of the study. The time in days to first brood for the controls and all treatment levels was 9.0 days. Therefore, no significant difference occurred in time to first brood for any test level. Based on the statistical analysis of survival, young/adult/repro duction day, adult mean length and length in days to first brood from this 21-day Daphnia magna dynamic life cycle study, the MATC limits were estimated to be 0.087 (NOEC) and 0.23 µg/l (LOEC) . The point estimate MATC value was 0.14 µg/l.
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