2-Propenoic acid, methyl ester, reaction products with 2-ethyl-1-hexanamine and sodium hydroxide

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects on inhaled methanol in pituitary and testicular hormones in chamber acclimated and non-acclimated rats.

Author:

Cooper, R.L. et al.

Year:

1992

Bibliographic source:

Toxicology 71: 69-81

Materials and methods

Principles of method if other than guideline:

Two experiments were conducted to evaluate the acute effects of inhaled methanol on serum hormones associated with reproductive function in male rats.

GLP compliance:

not specified

Test type:

other: actue effects and hormone status

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Methanol

EC Number:

200-659-6

EC Name:

Methanol

Cas Number:

67-56-1

Molecular formula:

CH4O

IUPAC Name:

methanol

Test material form:

liquid

Details on test material:

Purity: > 94%

Test animals

Species:

rat

Strain:

Long-Evans

Sex:

male

Administration / exposure

Route of administration:

inhalation

Type of inhalation exposure:

not specified

Vehicle:

other: unchanged (no vehicle)

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

>= 1 - <= 6 h

Concentrations:

0.27; 6.65 or 13.3 mg/L (corresponding to 200; 5000 or 10000 ppm)

No. of animals per sex per dose:

10

Control animals:

yes

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

other: hormone status

Effect level:

>= 0.27 - <= 13.3 mg/L air

Exp. duration:

6 h

Remarks on result:

other: increased prolactin concentrations

Any other information on results incl. tables

Serum luteinising hormone, testosterone and follicle stimulating hormone values were not different in sham- vs methanol-exposed rats at any time point. An effect of prior handling was noted: In general, the concentrations of these hormones and serum prolactin in the non-acclimated rats were greater than those observed for acclimated rats. However, methanol exposure appeared to result in increased prolactin concentrations under both handling conditions.

In conclusion, effects on serum hormone levels (luteinizing hormone, follicle stimulating hormone, testosterone, prolactine) were inconsistent with partly no effects and partly contradictory results. According the author methanol treatment led to increased prolactin levels in serum.