Divinylbenzene

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

of read across substance

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

WoE report is based on two short term toxicity study of fish for the test chemical :1.To evaluate antibacterial activity of test material2. Cell multiplication inhibition test was carried out to study the effects of test material on microorganism.

GLP compliance:

not specified

Specific details on test material used for the study:

- IUPAC Name: 1,2-di(ethenyl)benzene - Common Name: Divinylbenzene - InChI: 1S/C10H10/c1-3-9-7-5-6-8-10(9)4-2/h3-8H,1-2H2 - Smiles: C=Cc1ccccc1C=C - Molecular formula :C10H10 - Molecular weight :131.1969 g/mol - Substance type:Organic - Physical state:liquid

Analytical monitoring:

yes

Remarks:

1) Minimum inhibitory concn (MIC) for arithmetic difference between percentage transmittance (620 nm) of control (ethanol only) and test cultures were observed

Vehicle:

yes

Remarks:

1) Ethanol

Test organisms (species):

other: 1) Vibrio parahaemolyticus , 2) Pseudomonas putida

Test type:

static

Water media type:

freshwater

Total exposure duration:

9 h

Test temperature:

1) 30 deg C

Reference substance (positive control):

not specified

Duration:

9 h

Dose descriptor:

other: MIC

Effect conc.:

60 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Read across 1

Dose descriptor:

other: TT

Effect conc.:

62 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: Read across 2

Validity criteria fulfilled:

not specified

Conclusions:

The test chemical 1,2-di(ethenyl)benzene is likely to be toxic to microorganisms atleast in the concentration range of 60 - 62 mg/l

Executive summary:

Data available for the closely related read across chemicals has been reviewed to determine the short term toxicity of microorganisms of the test chemical 1,2-di(ethenyl)benzene (1321 -74 -0).The studies are as mentioned below:

1.Vibrio parahaemolyticus was exposed to test material for 9 hrs at 30°C at complex medium using Ethanol as a solvent .

 Minimum inhibition concentration was calculated using arithmetic difference between percentage transmittance (620 nm) of control (ethanol only) and test culture. Minimum inhibition concentration of Decanoic acid on Vibrio parahaemolyticus was observed to be 60mg/l.

2.Cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxcity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 62 mg/L.

Hence,based on the data available for the structurally and functionally similar read across, test chemical 1,2-di(ethenyl)benzene is not likely to be toxic to microorganisms atleast in the concentration range of 60- 62 mg/L .

Description of key information

Toxicity to microorganism:

Data available for the closely related read across chemicals has been reviewed to determine the short term toxicity of microorganisms of the test chemical 1,2-di(ethenyl)benzene (1321 -74 -0).The studies are as mentioned below:

1.Vibrio parahaemolyticus was exposed to test material for 9 hrs at 30°C at complex medium using Ethanol as a solvent .

 Minimum inhibition concentration was calculated using arithmetic difference between percentage transmittance (620 nm) of control (ethanol only) and test culture. Minimum inhibition concentration of Decanoic acid on Vibrio parahaemolyticus was observed to be 60mg/l.

2.Cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxcity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 62 mg/L.

Hence,based on the data available for the structurally and functionally similar read across, test chemical 1,2-di(ethenyl)benzene is not likely to be toxic to microorganisms atleast in the concentration range of 60- 62 mg/L .

Key value for chemical safety assessment

EC50 for microorganisms:

60 mg/L

Additional information

Toxicity to microorganism:

Data available for the closely related read across chemicals has been reviewed to determine the short term toxicity of microorganisms of the test chemical 1,2-di(ethenyl)benzene (1321 -74 -0).The studies are as mentioned below:

1.Vibrio parahaemolyticus was exposed to test material for 9 hrs at 30°C at complex medium using Ethanol as a solvent .

 Minimum inhibition concentration was calculated using arithmetic difference between percentage transmittance (620 nm) of control (ethanol only) and test culture. Minimum inhibition concentration of Decanoic acid on Vibrio parahaemolyticus was observed to be 60mg/l.

2.Cell multiplication inhibition test was carried out to study the effects of test material on Pseudomonas putida. The threshold toxcity of test material on cell multiplication inhibition effect on Pseudomonas putida was observed to be 62 mg/L.

Hence,based on the data available for the structurally and functionally similar read across, test chemical 1,2-di(ethenyl)benzene is not likely to be toxic to microorganisms atleast in the concentration range of 60- 62 mg/L .