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Diss Factsheets
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EC number: 236-195-6 | CAS number: 13223-03-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Toxicity to microorganisms
Administrative data
- Endpoint:
- toxicity to microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22/08/1991-25/08/1991
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented report of a guideline study conducted to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- yes
- Remarks:
- The growth characteristics of the bacteria were not measured by turbidimetr by using a microbiological method with Petri dish cultures according to Bringmann and Kühn, 1977.
- GLP compliance:
- yes (incl. QA statement)
Test material
- Details on test material:
- - Name of test material: Adame-Quat
- Physical state: liquid
- CAS number: 44992-01-0
- Manufacturing code: B4846
- Composition of test material, percentage of components: 80 % aqueous solution
- Lot/batch No.: 40034237
- pH-value: 6.56
- Storage condition of test material: at 4°C in the dark
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Demineralised water
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Laboratory culture: master cultures DSM N° 50026 supplied from "Deutsche Sammlung für Mikroorganismen, Göttinger, FRG
- Method of cultivation: Columbia agar (Api Bio Merieux, No 43041)
- Preparation of inoculum for exposure: Master cultures were incubated 1 day at 30°C 1 day at approximately 30°C and then stored in refrigerator at 4°C
- Initial biomass concentration: For the test the preliminary culture was diluted with sterile sodium chloride solution (0.9 %) to an extinction of 0.1 (at 436 nm) to gain the bacteria suspension.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 24 h
- Post exposure observation period:
- After one day of incubation, the growth of bacteria colonies on the petri dishes was evaluated macroscopically.
Test conditions
- Hardness:
- No data
- Test temperature:
- 30°C
- pH:
- 6.56
- Dissolved oxygen:
- No data
- Salinity:
- No data
- Nominal and measured concentrations:
- The nominal concentrations tested were 3, 6, 12, 24, 49, 98, 195, 391, 781, 1563, 3125, 6250, 12500, 25000, 50000, 100000, 200000, 400000 mg/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: 8.5cm Petri dishes
EFFECT PARAMETERS MEASURED: growth of bacteria colonies on the Petri dishes was evaluated macroscopically
TEST CONCENTRATIONS
- Spacing factor for test concentrations: dilution factor: 2
STATISTICS
The EC50-value was calculated as follows: The two concentrations which showed semi-maximal growth were added and then divided by two. The minimal inhibition concentration was calculated as the mean value between the concentration where no growth was observed and the concentration where minimal growth was observed. - Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 195 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 586 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
- Duration:
- 24 h
- Dose descriptor:
- IC100
- Effect conc.:
- 2 344 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- growth inhibition
Any other information on results incl. tables
The growth of the bacterial colonies on the Petri Dishes was evaluated macroscopically. The results are given in the following table:
Growth at dilution of 10-2 |
|||
Concentration |
Dilution factor |
Incubation A |
Incubation B |
control |
control |
+++ |
+++ |
400,000 |
1:2 |
– |
– |
200,000 |
1:4 |
– |
– |
100,000 |
1:8 |
– |
– |
50,000 |
1:16 |
– |
– |
25,000 |
1:32 |
– |
– |
12,500 |
1:64 |
– |
– |
6,250 |
1:128 |
– |
– |
3,125 |
1:256 |
– |
– |
1,563 |
1:512 |
+ |
+ |
781 |
1:1024 |
++ |
++ |
391 |
1:2048 |
++ |
++ |
195 |
1:4096 |
+++ |
+++ |
98 |
1:8192 |
+++ |
+++ |
49 |
1:16392 |
+++ |
+++ |
24 |
1:32784 |
+++ |
+++ |
12 |
1:65568 |
+++ |
+++ |
6 |
1:131136 |
+++ |
+++ |
3 |
1:262772 |
+++ |
+++ |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The results of this study indicate that the test substance Adame-Quat completely reduces bacteria growth at concentrations between 3125 and 400000 mg/L. No effect in comparison to the control was seen at a concentration of 195 mg/L which is considered to be the NOEC. The EC50 is 586 mg per litre and the MIC (EC100) is 2344 mg/L. Based on the results obtained in this test system, the test substance is considered to be slightly toxic to bacteria.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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