Phenol, 2-methoxy-, reaction products with 2,2-dimethyl-3-methylenebicyclo[2.2.1]heptane, hydrogenated

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2005

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Remarks:

EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05

Qualifier:

according to guideline

Guideline:

other: EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05

Deviations:

not specified

Principles of method if other than guideline:

see EPA/600/4-90/027, Methods for Measuring the Acute Toxicitv of Effluents and Receiving Waters to Freshwater Organisms, and by ASTM's Methods E729, 1997, Volume 11.05, Annual Book of ASTM Standards. Standard Guide for Conducting Acute Toxicitv Tests on Test Materials with Fishes, Macroinvertebrates, and Amphibians.

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

not specified

Details on test solutions:

The stock solutions used for the test solutions were prepared by diluting a known weight (grams) of the test material to a known volume. methanol as the solvent carrier was used.
Fourth Edition, by GLEC's (Great Lakes Environmental Center) Standard Operating Procedure for Stock Solution Preparation for Polymers (Detergents and Chemical Additives).

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: fathead minnows
- Strain: Pimephales promelas,
- Source: no data available
- Age at study initiation (mean and range, SD): less than 24 hours old
- Length at study initiation (length definition, mean, range and SD): no data available
- Weight at study initiation (mean and range, SD): no data available
- Method of breeding: no data available
- Maintenance of the brood fish: no data available

ACCLIMATION
- Acclimation period: no data available
- Acclimation conditions (same as test or not): no data available
- Type and amount of food during acclimation: no data available
- Feeding frequency during acclimation: no data available
- Health during acclimation (any mortality observed): no data available

QUARANTINE (wild caught)
- Duration: no data available
- Health/mortality: no data available

FEEDING DURING TEST
- Food type: no data available
- Amount: no data available
- Frequency: no data available

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Hardness:

no data available

Test temperature:

25 +/-1°C

pH:

no data available

Dissolved oxygen:

no data available

Salinity:

no data available

Conductivity:

no data available

Nominal and measured concentrations:

Nominal concentrations: 0.34; 0.68; 1.36; 2.73 and 5.45 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable):closed
- Material, size, headspace, fill volume: covered glass beakers containing at least 250 mL of the test solution or control
- Aeration: yes, The test solutions were aerated with a glass gas dispersion tube or a glass pipette at a rate that did not exceed 100 bubbles per minute
- Renewal rate of test solution (frequency/flow rate): 24 hour-renewal. . Toxicant/effluent renewal was performed daily; within two hours of the test start time.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): no data available
- Biomass loading rate: no data available

TEST MEDIUM / WATER PARAMETERS
No data available

OTHER TEST CONDITIONS
- Adjustment of pH: Sodium hydroxide and hydrochloric or sulfuric acids were used to adjust the pH of the test solution, if needed.
- Photoperiod: A 16-hour light: 8 hour dark photoperiod
- Light intensity: no data available

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Survival was checked daily and the dead fish were removed. Fish weight was measured at the end of the test.
LC50, NOEC and LOEC values were calculated based on adult survival, growth (weight)

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 0.5
- Range finding study
- Test concentrations: A range-finding toxicity test was conducted initially on each fragrance chemical at one to three times the estimated water solubility to determine the test concentrations for the definitive chronic toxicity tests.
- Results used to determine the conditions for the definitive study: Based on the results of the range-finding tests, we initiated the definitive chronic toxicity tests with fathead minnows using three times the estimated water solubility as the highest test concentration and a dilution factor of 0.5 to produce test concentrations.

Reference substance (positive control):

not specified

Key result

Duration:

7 d

Dose descriptor:

LC50

Effect conc.:

1.36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

0.34 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

weight

Key result

Duration:

7 d

Dose descriptor:

LOEC

Effect conc.:

1.36 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No other data

Results with reference substance (positive control):

no data available

Reported statistics and error estimates:

no data available

Validity criteria fulfilled:

yes

Remarks:

Survival and growth in the controls was determined; greater than 80 percent survival and average weight of greater than or equal to 0.250 mg/fish

Conclusions:

With a LC50-7days: 1.36 mg/L, isobornylcyclohexanol is toxic for fish.

Executive summary:

A static renewal fathead minnow (Pimephales promelas) chronic toxicity test (7-days) was performed with Iso bornyl cyclo hexanol according to EPA/600/4-90/027 and ASTM's Methods E729, 1997, Volume 11.05.

Results:

- LC50: 1.36 mg/L

-       NOEC: 0.34 mg/L (based on growth)

-       LOEC: 1.36 mg/L (based on survival)

With a LC50-7days: 1.36 mg/L, isobornylcyclohexanol is toxic for fish.

Description of key information

The test item, isobornylcyclohexanol, was considered toxic to Pimephales promelas.

The 7-d LC50 of isobornylcyclohexanol to Pimephales promelas was determined to be 1.36 mg/L based on the mobility; revealing it is toxic for the fish.

Additional information

A study, scored as Klimisch 2 and flagged as a key study, is available on isobornylcyclohexanol, giving a 7-d-LC50 : 1.36 mg/L and revealing that isobornylcyclohexanol is toxic to fish (S.C. Johnson, 2005).