2,2,4(or 2,4,4)-trimethylhexane-1,6-diamine

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988-06-15 to 1988-06-17

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedure in accordance with national standard methods with acceptable restrictions: Exposure period only 24 hours, incomplete documentation

Qualifier:

according to guideline

Guideline:

other: DIN 38412 part 11

Deviations:

not specified

GLP compliance:

no

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Concentration: 10 g/l, no solvent or vehicle

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISMS
- Strain: Daphnia magna, Hüls
- Source/supplier: Hüls AG (inhouse)
- Breeding method: in 1 l jars with dechlorinated drinking water, water  renewal each 2-3 days, isolation of juveniles for further breeding ca every
4 weeks
- Age: < 24 hours
- Feeding: Chlorella vulgaris, daily as much as consumed
- Pretreatment: Removal of adults 24 h prior to testing
- Feeding during test: no

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Test temperature:

20 +/- 1 degree C

Nominal and measured concentrations:

12; 18; 25; 35; 50; 70; 100 mg/l (nominal)

Details on test conditions:

TEST SYSTEM
- Exposure vessel type: 25 ml graduated cylinder
- Number of replicates, individuals per replicate: 4 replicates with 5 individuals each
- Control group: 2 reference substance controls, one blank
- Photoperiod: dark

DILUTION WATER
- Source: Synthetic: CaCl2 x 2 H2O: 294 mg/l; MgSO4 x 7 H2O: 123 mg/l; NaHCO3: 63 mg/l; KCl: 5.5 mg/l
- Ca/Mg ratio: 4:1
- Na/K ratio: 10:1
- Aeration: no

TEST PARAMETER: immobilization

Reference substance (positive control):

yes

Remarks:

potassium dichromate, CAS RN 7778-50-9

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

31.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 28.9-34.2

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

70 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- EC50 values were calculated according to Cavalli-Sforza (1972)
- the mortality data are presented below (remarks on results)

Results with reference substance (positive control):

- Concentrations: 0.9 mg/l / 1.9 mg/l
- Results: 30 % immobile / 85 % immobile (24 hours)

RESULTS: 
- Effect data (Immobilisation): No. of immobile daphnia
  Control:   0 (24 h)
   12 mg/l:  0 (24 h) 
   18 mg/l:  0 (24 h)
   25 mg/l:  2 (24 h)
   35 mg/l: 16 (24 h)
   50 mg/l: 19 (24 h) 
   70 mg/l: 20 (24 h) 
  100 mg/l: 20 (24 h) 
  EC50, 95 % confidence interval: 28.9 - 34.2 mg/l (24 hours)

Validity criteria fulfilled:

not specified

Conclusions:

The EC50 (24 hours) was determined as 31.5 mg/L indicating that the test substance may be harmful to aquatic invertebrates.

Executive summary:

The acute toxicity of the test item on Daphnia magna was investigated by Hüls AG (1996) in a 24 -h test according to national guidance documents (DIN 38412, part II). The following endpoint was derived:

EC50 (24 hours): 31.5 mg/L; CL: 28.9 mg/L - 34.2 mg/L.

The study was assessed as "reliable with restrictions".

Description of key information

The acute toxicity of the test item on Daphnia magna was investigated by Hüls AG (1996) in a 24 -h test according to national guidance documents (DIN 38412, part II). The EC50 (24 hours) was determined as 31.5 mg/L (CL: 38.9 - 34.2 mg/L) indicating that the test substance may be harmful to 
aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

31.5 mg/L