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EC number: 214-540-1 | CAS number: 1143-72-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 27 Feb - 15 Mar 1985
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 985
- Report date:
- 1985
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Version / remarks:
- adopted Jul 1997
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- 2,3,4-trihydroxybenzophenone
- EC Number:
- 214-540-1
- EC Name:
- 2,3,4-trihydroxybenzophenone
- Cas Number:
- 1143-72-2
- Molecular formula:
- C13H10O4
- IUPAC Name:
- 4-benzoylbenzene-1,2,3-triol
- Test material form:
- solid
Constituent 1
Method
- Target gene:
- his operon and trp operon
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- cofactor supplemented post-mitochondrial fraction (S9 mix), prepared from the livers of rats treated with Aroclor 1254.
- Test concentrations with justification for top dose:
- Pre-experiment: 4, 20, 100, 500, 2500 and 10000 µg/plate with and without metabolic activation
Main Experiment:
Experiment I:
The pre-experiment served as Experiment I.
Experiment II:
4, 20, 100, 500, 2500 and 5000 µg/plate with and without metabolic activation - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controls
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- benzo(a)pyrene
- other: -S9: 2-nitrofluorene (5 µg/plate) for TA1538 and TA98; N-Methyl-N'-nitro-N-nitrosoguanidine (2.5 µg/plate) for WP2uvrA; +S9: 2-aminoanthracene (0.5, 1 or 10 µg/plate) for all strains
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 - 72 h
NUMBER OF REPLICATIONS: 2 and 3 replications each in 2 independent experiments (Experiment 1 and 2), respectively.
DETERMINATION OF CYTOTOXICITY
- Method: A reduced rate of spontaneously occuring colonies and visible thinning of the bacterial lawn were used as indicator for toxicity.
OTHER EXAMINATIONS:
Sterility check: A sterility check was performed with agar plates only containing the test substance with and without S9 mix. - Statistics:
- Mean values were calculated
Results and discussion
Test resultsopen allclose all
- Key result
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment 1: ≥ 2500 µg/plate without metabolic activation and ≥ 500 µg/plate with metabolic activation; Experiment 2: ≥ 2500 µg/plate with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment 1 and 2: ≥ 2500 µg/plate with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment 1 and 2: ≥ 2500 µg/plate with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment 1 and 2: ≥ 2500 µg/plate with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment 1 and 2: ≥ 2500 µg/plate with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Key result
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- Experiment 1 and 2: ≥ 2500 µg/plate with and without metabolic activation
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- TEST-SPECIFIC CONFOUNDING FACTORS
- Precipitation: Precipiation of the test substance on the plates was observed in the highest concentration of 10000 µg/plate with and without metabolic activation.
RANGE-FINDING/SCREENING STUDIES: The pre-experiment served as Experiment I (all strains were tested in the pre-experiment).
HISTORICAL CONTROL DATA
- Positive historical control data: The historical control data of the positive controls were not provided in the study report, but the control plates with the background control gave the expected number of colonies.
- Negative (solvent/vehicle) historical control data: No historical control data of the vehicle control was provided in the study report, but the control plates with the positive controls gave the expected number of colonies.
OTHER EXAMINATIONS:
Sterility of S-9 Mix and the test compound was indicated by the absence of contamination on the test material and S-9 Mix sterility check plates.
Any other information on results incl. tables
Table 1: Summary of Experiment 1
EXPERIMENT 1 | ||||||
S9-Mix | Without | |||||
Test item (µg/plate) | TA100 | TA1535 | TA1537 | TA1538 | TA98 | WP2uvrA |
0 | 144 | 19 | 10 | 12 | 27 | 16 |
4 | 164 | 18 | 8 | 10 | 19 | 27 |
20 | 161 | 14 | 11 | 13 | 24 | 22 |
100 | 175 | 14 | 8 | 13 | 27 | 22 |
500 | 160 | 11 | 7 | 14 | 23 | 17 |
2500 | 9 (ibl) | ibl | nbl | 0 ibl | 0 (ibl) | 4 (ibl) |
10000 | 0 P (nbl) | 0 P (nbl) | 0 P (nbl) | 0 P (nbl) | 0 P (nbl) | 0 P (nbl) |
Positive controls | SA | SA | 9-AA | 2-NF | 2-NF | MNNG |
Concentrations (μg/plate) | 1 | 1 | 50 | 5 | 5 | 2.5 |
Mean No. of colonies/plate | 560 | 384 | 95 | 786 | 450 | 221 |
S9-Mix | With | |||||
Test item (µg/plate) | TA100 | TA1535 | TA1537 | TA1538 | TA98 | WP2uvrA |
0 | 145 | 15 | 12 | 16 | 27 | 25 |
4 | 174 | 13 | 11 | 13 | 31 | 23 |
20 | 185 | 13 | 9 | 18 | 31 | 19 |
100 | 196 | 12 | 15 | 17 | 35 | 25 |
500 | 130 (ibl) | 7 | 11 | 17 | 30 | 19 |
2500 | 23 (ibl) | 2 (ibl) | 0 (ibl) | 0 (ibl) | 1 (ibl) | 4 |
10000 | 0 P (nbl) | 0 P (nbl) | 0 P (ibl) | 0 P (nbl) | 0 P (nbl) | 0 P (nbl) |
Positive control | 2-AA | 2-AA | 2-AA | 2-AA | 2-AA | 2-AA |
Concentrations (μg/plate) | 0.5 | 1 | 1 | 0.5 | 0.5 | 10 |
Mean No. of colonies/plate | 680 | 156 | 109 | 538 | 545 | 272 |
Positive control | B(a)P | B(a)P | B(a)P | B(a)P | B(a)P | B(a)P |
Concentrations (μg/plate) | 10 | 10 | 10 | 10 | 10 | 10 |
Mean No. of colonies/plate | 449 | 29 | 163 | 269 | 704 | 57 |
ibl: incomplete background lawn in all plates
(ibl): incomplete background lawn in individual plates
nbl: no background lawn
(nbl): no background lawn in individual plates
P: precipitation
SA: sodium azide
9-AA: 9 -aminoacridine
2-NF: 2 -nitrofluorene
MNNG: N-methyl-N'-nitro-N-nitrosoguanidine
2-AA: 2 -aminoanthracen
B(a)P: benzo(a)pyrene
Table 2: Summary of Experiment 2
EXPERIMENT 2 | ||||||
S9-Mix | Without | |||||
Test item (µg/plate) | TA100 | TA1535 | TA1537 | TA1538 | TA98 | WP2uvrA |
0 | 134 | 21 | 13 | 16 | 27 | 20 |
4 | 151 | 18 | 14 | 17 | 31 | 23 |
20 | 160 | 22 | 11 | 8 | 26 | 18 |
100 | 199 | 20 | 11 | 14 | 25 | 19 |
500 | 164 | 17 | 15 | 17 | 27 | 20 |
2500 | 0 (nbl) | nbl | 0 (ibl) | 0 (ibl) | 1 (ibl) | 1 (ibl) |
5000 | 0 (nbl) | 0 (nbl) | 0 (nbl) | 0 (nbl) | 0 (nbl) | 0 (nbl) |
Positive controls | SA | SA | 9-AA | 2-NF | 2-NF | MNNG |
Concentrations (μg/plate) | 1 | 1 | 50 | 5 | 5 | 2.5 |
Mean No. of colonies/plate | 601 | 413 | 132 | 192 | 627 | 593 |
S9-Mix | With | |||||
Test item (µg/plate) | TA100 | TA1535 | TA1537 | TA1538 | TA98 | WP2uvrA |
0 | 143 | 17 | 12 | 13 | 27 | 19 |
4 | 157 | 9 | 11 | 17 | 28 | 16 |
20 | 191 | 12 | 15 | 17 | 35 | 21 |
100 | 193 | 11 | 15 | 17 | 34 | 18 |
500 | 131 (ibl) | 11 | 16 | 13 | 36 | 19 |
2500 | 0 (ibl) | 1 (ibl) | 0 (ibl) | 2 (ibl) | 3 (ibl) | 7 (ibl) |
5000 | 0 (nbl) | 0 (nbl) | 0 (nbl) | 0 (nbl) | 0 (nbl) | 0 (nbl) |
Positive control | 2-AA | 2-AA | 2-AA | 2-AA | 2-AA | 2-AA |
Concentrations (μg/plate) | 0.5 | 1 | 1 | 0.5 | 0.5 | 10 |
Mean No. of colonies/plate | 2862 | 246 | 463 | 2520 | 2740 | 324 |
Positive control | B(a)P | B(a)P | B(a)P | B(a)P | B(a)P | B(a)P |
Concentrations (μg/plate) | 10 | 10 | 10 | 10 | 10 | 10 |
Mean No. of colonies/plate | 635 | 30 | 145 | 245 | 734 | 70 |
ibl: incomplete background lawn in all plates
(ibl): incomplete background lawn in individual plates
nbl: no background lawn
(nbl): no background lawn in individual plates
P: precipitation
SA: sodium azide
9-AA: 9 -aminoacridine
2-NF: 2 -nitrofluorene
MNNG: N-methyl-N'-nitro-N-nitrosoguanidine
2-AA: 2 -aminoanthracen
B(a)P: benzo(a)pyrene
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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