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EC number: 701-135-4 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- DIN 38412-8 (Pseudomonas Zellvermehrungshemmtest)
- Deviations:
- no
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Reaction mass of 1-(2,3-epoxypropoxy)-2,2-bis ((2,3-epoxypropoxy)methyl) butane and 1-(2,3-epoxypropoxy)-2-((2,3-epoxypropoxy)methyl)-2-hydroxymethyl butane
- EC Number:
- 701-135-4
- Cas Number:
- 30499-70-8
- Molecular formula:
- C15H26O6;C15H22O5
- IUPAC Name:
- Reaction mass of 1-(2,3-epoxypropoxy)-2,2-bis ((2,3-epoxypropoxy)methyl) butane and 1-(2,3-epoxypropoxy)-2-((2,3-epoxypropoxy)methyl)-2-hydroxymethyl butane
- Test material form:
- other: liquid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Vehicle:
- no
- Details on test solutions:
- A stock solution (emulsion) of 12,500 mg per litre in Milli-Q water (tap water purified by reverse osmosis and subsequently passed over activated
carbon and ion-exchange cartridges (Millipore Corp., Bedford, Mass., USA)) was prepared. Ultra sonication and thorough mixing were performed to maximise the dissolving of the test substance. No additives were used.
The pH of the stock solution was 7.9.
A dilution series of the test substance stock solution was prepared.
Test organisms
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- Pseudomonas putida. Strain MIGULA, Berlin 33/2 (DSM 50026).
Source: BGA, Berlin, Germany.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 18 h
Test conditions
- Test temperature:
- 23 +/- 1°C
- Nominal and measured concentrations:
- 313 - 10,000 mg/l (nominal)
- Details on test conditions:
- EXPERIMENTAL CONDITIONS AND SOLUTIONS
Vessels: 100 ml Erlenmeyer flasks
Incubation temperature: 23 +/- 1°C
Incubation time:
- preliminary suspension cultures: 6 +/- 1 hours
- exposure: 18 +/- 2 hours
Stock solution I:
10.0 g NaN03
2.4 g K2HP04
1.2 g KH2P04
1.0 g yeast extract
Dissolved in 500 ml Milli-Q water.
Stock solution II:
10.0 g NaN03
2.4 g K2HP04
1.2 g KH2P04
Dissolved in 500 ml Milli-Q water.
Stock solution III:
40.0 g D(+)- glucose
Dissolved in 500 ml Milli-Q water.
Stock solution IV:
0.01 g Fe (III) citrate
4.0 g MgSD4 . 7H20
Dissolved in 1 1 Milli-Q water.
Culture medium (stock)
Nutrient medium: 18 g of agar dissolved in water, plus 50 ml of solution I, 125 ml of solution III and 100 ml of solution IV, made up to one litre with water. - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 18 h
- Dose descriptor:
- EC10
- Effect conc.:
- 6 310 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 18 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 10 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Results with reference substance (positive control):
- The EC50 value of 3,5-dichlorophenol determined for Pseudomonas putida as calculated by linear regression (Y = 3.8772 X -62.5430) was 29 mg/l.
- Reported statistics and error estimates:
- After incubation extinction values were measured and the cell multiplication inhibition for each test concentration was determined.
When the concentration of the test substance was correlated with the corresponding inhibition by linear regression analysis, the following
equation was obtained:
Y = 14.4333 x - 44.7633
Where x represents the log concentration of TKK 30174, and Y the % inhibition.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Based on the results of the test presently performed, the toxicity threshold value (EC10) of TKK 30174 was 6310 mg/l.
The EC50 was over 10,000 mg/l. - Executive summary:
TKK 30174, ARALDITE DY 0396 was investigated for its ability to inhibit the cell multiplication of the bacteria species Pseudomonas putida according to DIN 38412 Part L 8 (March 1991) and NEN-EN-ISO 10712 (1996). Suspensions of Pseudomonas putida were exposed to concentrations ranging from 312.5 to 10,000 mg TKK 30174 per litre. After incubation the extinction values of the bacterial cell suspensions were measured. Based on the results of the test presently performed, the toxicity threshold value (EC10) of TKK 30174 was 6310 mg/1. The EC50 was over 10,000 mg/l.
The EC50 value for the reference substance, 3,5-dichlorophenol, tested during the same experiment was 29 mg/1. Since this was within the accepted range of 10-30 mg/1 for the EC50 and the multiplication factor of the bacteria within the test was over 60, it was concluded that the test conditions were optimal and the results obtained were valid.
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