Ethylenediamine-N,N'-di(acetic acid)

Toxicity to soil macroorganisms except arthropods

Link to relevant study record(s)

Reference

Endpoint:

toxicity to soil macroorganisms except arthropods: short-term

Type of information:

experimental study

Adequacy of study:

key study

Study period:

no data available

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well documented study, without GLP

Justification for type of information:

Study is read across and slightly updated and presented under "type of information" as experimental study until the lead company has updated the Source RSS
Read across justification is presented in IUCLID chapter 13

Qualifier:

according to guideline

Guideline:

OECD Guideline 207 (Earthworm, Acute Toxicity Tests)

GLP compliance:

not specified

Remarks:

Data published in open literature

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
no details available

Analytical monitoring:

not specified

Details on sampling:

no data

Vehicle:

no

Details on preparation and application of test substrate:

EDTA were applied in deionized water solution into artificial soil (35 % dry weight moisture content).

Test organisms (species):

Eisenia fetida

Animal group:

annelids

Details on test organisms:

no data available

Study type:

laboratory study

Substrate type:

artificial soil

Limit test:

no

Total exposure duration:

14 d

Post exposure observation period:

no data available

Test temperature:

20 °C

pH:

no data available

Moisture:

35 % of maximum holding capacity

Details on test conditions:

Glass jars were used as test containers. 750 g of artificial soil which had been treated with one of the test chemicals (or a control with no chemicals) were placed in each jar. Ten E. fetida each with live weights of approximately 1 mg were added to each jar. There were four replicates of each chemical and dose.

Nominal and measured concentrations:

0, 0.1, 1.0, 10.0, 100.0, 1000.0 and 10000.0 mg/KG dry weight soil.

Reference substance (positive control):

not specified

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

156.46 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 136.01 - 179.98

Results with reference substance (positive control):

no data available

Reported statistics and error estimates:

Data were analyzed statistically by analysis of variance using S.A.S. software.
The LD50 values were estimated using Trimmed Spearman–Karber Version 1.5 (S.E.P.A. Cincinnati, Ohio) designed for analysis of mortality data from acute chronic toxicity tests.

Validity criteria fulfilled:

not specified

Conclusions:

The 14 day LC50 of EDTA to earthworms (Eisenia foetida) was calculated to be 156 mg/kg dry weight soil.

Executive summary:

The 14 day LC50 of H4 EDTA to earthworms (Eisenia foetida) was calculated to be 156 mg/kg dry weight soil.

Description of key information

Only one acute toxicity study with earthworms is available for H4 EDTA (Edwards, 2009). The EC50 was determined at 156 mg/kg soil dry weight.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:

156 mg/kg soil dw

Additional information

The hazard assessment of H4 EDTA reveals neither a need to classify the substance as dangerous to the environment, nor is it a PBT or vPvB substance. The substance is expected to have a low potential for adsorption based on its ionic structure under environmental relevant pH conditions and its low log Kow , the low log Kow also indicates that the substance will not be bioaccumulative. There are no further indications that the substance may be hazardous to the environment.